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BNIP3 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS807411

BNIP3 is an apoptosis-inducing protein which can overcome BCL2 suppression. It may play a role in repartioning calcium between the two major intracellular calcium stores in association with BCL2. It has been shown to interact with CD47, BCL2-like, and BCL-2… More details

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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Product Overview

Product Name
BNIP3, Polyclonal Antibody
Full Product Name
Rabbit Anti-Human BNIP3 Polyclonal
Product Gene Name
anti-BNIP3 antibody
Product Synonym Names
NIP3 Antibody; BCL2 Adenovirus E1B 19kDa Interacting Protein 3 Antibody; BNIP3_Human Antibody; BNIP3 Antibody: ATTO 488; Rabbit Anti-Human BNIP3 Polyclonal; BNIP3 Antibody
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse
Purity/Purification
Peptide Affinity Purified
Concentration
1 mg/ml (lot specific)
Preparation and Storage
Store at -20 degree C
Molecular Weight
Predicted molecular weight at ~21.5kDa. Observed bands at ~20-30kDa and 50-60kDa due to the homodimer complex of BNIP3.
OMIM
603293
3D Structure
ModBase 3D Structure for Q12983
Immunogen
Synthetic peptide from the C-terminal of human BNIP3
Immunogen Species
Human
COA
A 1:1000 dilution was sufficient for detection of BNIP3 on mouse kidney lysates using Goat anti-rabbit IgG:HRP as the secondary antibody.
Storage Buffer
PBS, 50% glycerol, 0.09% sodium azide
Conjugate
ATTO 488
Cellular Localization
Mitochondrion, Mitochondrion outer membrane

Product Categories/Family for anti-BNIP3 antibody

Cancer; Apoptosis; Cardiovascular System; Heart
Applications Tested/Suitable for anti-BNIP3 antibody
Western Blot (WB)
Application Notes for anti-BNIP3 antibody
Western Blot: 1:1000

Western Blot (WB) of anti-BNIP3 antibody

Western blot analysis of Human HeLa and HEK293T cell lysates showing detection of ~30kDa BNIP3 protein using Rabbit Anti-BNIP3 Polyclonal Antibody (SPC-685). Lane 1: MW Ladder. Lane 2: Human HeLa (20 ug). Lane 3: Human 293T (20 ug). Load: 20 ug. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-BNIP3 Polyclonal Antibody (SPC-685) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~30kDa .
Rabbit Anti-BNIP3 Polyclonal Antibody – Cat# MBS807411

Western Blot (WB) of anti-BNIP3 antibody

Western blot analysis of Mouse Kidney showing detection of ~30kDa BNIP3 protein using Rabbit Anti-BNIP3 Polyclonal Antibody (SPC-685). Lane 1: MW Ladder. Lane 2: Mouse Kidney (20 ug). Load: 20 ug. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-BNIP3 Polyclonal Antibody (SPC-685) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~30kDa . Other Band(s): ~60kDa (BNIP3 complex).
Rabbit Anti-BNIP3 Polyclonal Antibody, Western Blot (WB) – Cat# MBS807411

Product References and Citations for anti-BNIP3 antibody

1. Boyd J.M., et al. (1994) Cell. 79(2): 341-351. 2. Lamy L., et al. (2003) J Biol Chem. 278(26): 23915-23921. 3. Ray R., et al. (2000) J Biol Chem. 275(2): 1439-1448.

Other Notes

Small volumes of anti-BNIP3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for BNIP3 purchase

MBS807411 is an antibody targeting BNIP3. Also known as Rabbit Anti-Human BNIP3 Polyclonal. Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for BNIP3. It may not necessarily be applicable to this product.

NCBI GI #
734703979
NCBI GeneID
664
NCBI Accession #
NP_004043.3
UniProt Primary Accession #
Q12983
UniProt Secondary Accession #
O14620; Q96GP0
UniProt Related Accession #
Q12983
GenBank Nucleotide #
NM_004052.3
NCBI Official Full Name
BCL2/adenovirus E1B 19 kDa protein-interacting protein 3
NCBI Official Synonym Full Names
BCL2/adenovirus E1B 19kDa interacting protein 3
NCBI Official Symbol
BNIP3
NCBI Official Synonym Symbols
NIP3
UniProt Protein Name
BCL2/adenovirus E1B 19 kDa protein-interacting protein 3
Protein Family
BCL2/adenovirus E1B 19 kDa protein-interacting protein
UniProt Gene Name
BNIP3
UniProt Synonym Gene Names
NIP3
UniProt Entry Name
BNIP3_HUMAN
NCBI Protein Information
BCL2/adenovirus E1B 19 kDa protein-interacting protein 3

NCBI Summary for BNIP3

This gene is encodes a mitochondrial protein that contains a BH3 domain and acts as a pro-apoptotic factor. The encoded protein interacts with anti-apoptotic proteins, including the E1B 19 kDa protein and Bcl2. This gene is silenced in tumors by DNA methylation. [provided by RefSeq, Dec 2014]

UniProt Comments for BNIP3

BNIP3: Apoptosis-inducing protein that, which can overcome BCL2 suppression. May play a role in repartitioning calcium between the two major intracellular calcium stores in association with BCL2. Homodimer. Binds to BCL2. Interacts with BNIP3L and ACAA2. Also can interact with adenovirus E1B 19 kDa protein or Epstein-Barr virus BHRF1. Belongs to the NIP3 family.

Protein type: Membrane protein, integral; Mitochondrial

Chromosomal Location of Human Ortholog: 10q26.3

Cellular Component: cytoplasm; dendrite; endoplasmic reticulum; integral to mitochondrial outer membrane; mitochondrial membrane; mitochondrial outer membrane; mitochondrion; nuclear envelope; nucleoplasm; nucleus

Molecular Function: GTPase binding; identical protein binding; protein binding; protein heterodimerization activity; protein homodimerization activity

Biological Process: apoptosis; autophagic cell death; brown fat cell differentiation; cell death; defense response to virus; induction of apoptosis by granzyme; mitochondrial fragmentation during apoptosis; mitochondrion degradation; negative regulation of apoptosis; negative regulation of membrane potential; neuron apoptosis; positive regulation of apoptosis; positive regulation of autophagy; positive regulation of macroautophagy; positive regulation of programmed cell death; positive regulation of protein complex disassembly; regulation of mitochondrial membrane permeability; response to hyperoxia; response to hypoxia; viral reproduction
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Research Articles on BNIP3

1. phosphorylation of these C-terminal BNIP3 residues blocks cell death without preventing autophagy, providing evidence that the two functional roles of BNIP3 can be regulated independently

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-BNIP3 antibody

 Reference Product  PubMed Publications
 HIF1A antibody  >130 publications with BNIP3 and HIF1A
 BCL2 antibody  >111 publications with BNIP3 and BCL2
 BNIP3L antibody  >110 publications with BNIP3 and BNIP3L
 FOXO3 antibody  >28 publications with BNIP3 and FOXO3
 TNF antibody  >22 publications with BNIP3 and TNF
 MAP1LC3B antibody  >9 publications with BNIP3 and MAP1LC3B
 ARNT antibody  >5 publications with BNIP3 and ARNT
 RHEB antibody  >3 publications with BNIP3 and RHEB
 HRAS antibody  >2 publications with BNIP3 and HRAS

Pathways associated with anti-BNIP3 antibody

 Products by Pathway  Pathway Diagram
 Apoptosis Modulation And Signaling Pathway antibodies  Apoptosis Modulation And Signaling Pathway Diagram
 FoxO Signaling Pathway antibodies  FoxO Signaling Pathway Diagram
 HIF-1-alpha Transcription Factor Network Pathway antibodies  HIF-1-alpha Transcription Factor Network Pathway Diagram
 Legionellosis Pathway antibodies  Legionellosis Pathway Diagram
 Legionellosis Pathway antibodies  Legionellosis Pathway Diagram
 MTOR Signaling Pathway antibodies  MTOR Signaling Pathway Diagram

Diseases associated with anti-BNIP3 antibody

 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >84 publications with BNIP3 and Cardiovascular Diseases
 Nervous System Diseases Antibodies  >73 publications with BNIP3 and Nervous System Diseases
 Necrosis Antibodies  >62 publications with BNIP3 and Necrosis
 Heart Diseases Antibodies  >46 publications with BNIP3 and Heart Diseases
 Brain Diseases Antibodies  >44 publications with BNIP3 and Brain Diseases
 Breast Neoplasms Antibodies  >40 publications with BNIP3 and Breast Neoplasms
 Disease Models, Animal Antibodies  >35 publications with BNIP3 and Disease Models, Animal
 Inflammation Antibodies  >22 publications with BNIP3 and Inflammation
 Myocardial Infarction Antibodies  >20 publications with BNIP3 and Myocardial Infarction
 Neoplasms, Experimental Antibodies  >19 publications with BNIP3 and Neoplasms, Experimental

Organs/Tissues associated with anti-BNIP3 antibody

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >141 publications with BNIP3 and Muscle
 Heart Antibodies  >105 publications with BNIP3 and Heart
 Vascular Antibodies  >72 publications with BNIP3 and Vascular
 Brain Antibodies  >70 publications with BNIP3 and Brain
 Blood Antibodies  >64 publications with BNIP3 and Blood
 Lung Antibodies  >36 publications with BNIP3 and Lung
 Liver Antibodies  >35 publications with BNIP3 and Liver
 Bone Antibodies  >24 publications with BNIP3 and Bone
 Kidney Antibodies  >21 publications with BNIP3 and Kidney
 Embryonic Tissue Antibodies  >13 publications with BNIP3 and Embryonic Tissue
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