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Arginylation Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS808394

Protein arginylation is the post-translational addition of arginine to proteins by arginyltransferase ATE1. Arginylation of proteins has been found to play an essential role in physiological pathways during embryogenesis and adulthood (1). Arginylation has… More details

$630 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Arginylation, Monoclonal Antibody
Full Product Name
N-terminal Arginylation Antibody, Clone 4D12
Product Synonym Names
Mouse Anti-N-terminal Arginylation Monoclonal IgG1; N-terminal Arginylation Antibody; N-terminal Arginine Antibody; N-terminal Arginylation Antibody; N-terminal Arginylated Antibody; N terminal Arginine Antibody; N terminal Arginylation Antibody; N terminal Arginylated Antibody; Amino-terminal Arginine Antibody; Amino-terminal Arginylation Antibody; Amino-terminal Arginylated Antibody; Amino terminal Arginine Antibody; Amino terminal Arginylation Antibody; Amino terminal Arginylated Antibody
Clonality
Monoclonal
Isotype
IgG1
Clone Number
4D12
Host
Mouse
Species Reactivity
Species Independent
Purity/Purification
Protein G Purified
Concentration
1 mg/ml (lot specific)
Specificity
Specific for N-terminal arginine, next to both glutamic acid and aspartic acid. Does not detect internal arginine.
Preparation and Storage
Store at -20 degree C.
Matching Pairs
Matching Pairs
Unconjugated Antibody: Arginylation Clone #4D12 (MBS808394)
ATTO 390 Conjugated Antibody: Arginylation Clone #4D12 (MBS808395)
Target
N-terminal Arginylation
Immunogen
Synthetic N-terminal arginylated peptide conjugated to KLH
Cellular Localization
Endoplasmic Reticulum
Conjugate
Unconjugated
Storage Buffer
PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide
Applications Tested/Suitable for anti-Arginylation antibody
Western Blot (WB)
Application Notes for anti-Arginylation antibody
WB: 1:1000

Western Blot (WB) of anti-Arginylation antibody

Western Blot analysis of N-terminal Arginine-BSA showing detection of 67 kDa N-terminal Arginylation protein using Mouse Anti-N-terminal Arginylation Monoclonal Antibody, Clone 4D12 . Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA. Lane 3: RDHKH-BSA. Lane 4: REHKH-BSA. Lane 5: HKH-BSA. Lane 6: HKERD-BSA. Lane 7: HKRRE-BSA. Load: 0.5 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-N-terminal Arginylation Monoclonal Antibody at 1:1000 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 2 hour at RT. Color Development: ECL solution (Super Signal West Pico) for 5 min in RT. Predicted/Observed Size: 67 kDa. Other Band(s): 250kDa, 75kDa, 20kDa RDHKH-BSA; 250kDa, 75kDa REHKH-BSA.
Mouse Anti-Arginylation Monoclonal Antibody – Cat# MBS808394

Product References and Citations for anti-Arginylation antibody

1. Saha S. and Kashina A. (2011) Dev Biol. 385(1): 1-8. 2. Deka K., et al. (2016) Cell Death Discov. 2: 16074.

Other Notes

Small volumes of anti-Arginylation antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Arginylation purchase

MBS808394 is an antibody targeting Arginylation. Also known as N-terminal Arginylation Antibody, Clone 4D12. Reactive in Species Independent. Host: Mouse.
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