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ERp57 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS808445

ERp57, also known as Glucose Regulated Protein 58 (Grp58), Hormone-Induced Protein-70 (HIP-70) and microsomal Carnitine Palmitoyltransferase, is a member of the protein disulfide isomerase family, containing two canonical CXHC tetrapeptide active site motifs… More details

$575 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
ERp57, Monoclonal Antibody
Full Product Name
ERp57 Antibody, Clone 4F9: PerCP
Product Gene Name
anti-ERp57 antibody
Product Synonym Names
Mouse Anti-Human ERp57 Monoclonal IgG2b; ERp57 Antibody: PerCP; ERp60 Antibody; ERp61 Antibody; Grp57 Antibody; Grp58 Antibody; P58 Antibody; PDIA3 Antibody; PI PLC Antibody; 58 kDa glucose regulated protein Antibody; 58 kDa glucose-regulated protein Antibody; 58 kDa microsomal protein Antibody; Disulfide isomerase ER 60 Antibody; Disulfide isomerase ER-60 Antibody; Endoplasmic reticulum resident protein 57 Antibody; Endoplasmic reticulum resident protein 60 Antibody; ER p57 Antibody; ER protein 57 Antibody; ER protein 60 Antibody; ERp 57 Antibody; ERp57 Antibody; Glucose Regulated Protein 58 Kd Antibody; GRP 57 Antibody; GRP 58 Antibody; GRP57 Antibody; HsT17083 Antibody; p58 Antibody; PDIA 3 Antibody; PDIA3 Antibody; PDIA3_HUMAN Antibody; Phospholipase C alpha Antibody; PI PLC Antibody; Protein disulfide isomerase A3 Antibody; Protein disulfide isomerase family A member 3 Antibody; Protein disulfide-isomerase A3 Antibody
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
4F9
Host
Mouse
Species Reactivity
Human
Purity/Purification
Protein G Purified
Concentration
1 mg/ml (lot specific)
Specificity
Detects ~57 kDa protein.
Preparation and Storage
Store at -20 degree C.
Matching Pairs
Unconjugated Antibody: ERp57 Clone #4F9 (MBS808430)
PerCP Conjugated Antibody: ERp57 Clone #4F9 (MBS808445)
Matching Pairs
Unconjugated Antibody: N/A (MBS808445)
PerCP Conjugated Antibody: ERp57 Clone #4F9 (MBS808445)
Molecular Weight
56,782 Da
OMIM
602046
3D Structure
ModBase 3D Structure for P30101
Target
ERp57
Immunogen
Human recombinant ERp57 (Grp58) protein corresponding to amino acids 25- 505 (his-tagged)
Immunogen Species
Human
Cellular Localization
Endoplasmic Reticulum; Endoplasmic Reticulum Lumen; Melanosome
Conjugate
PerCP
Storage Buffer
PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide

Product Categories/Family for anti-ERp57 antibody

Antibodies; Cell Signaling; Protein Trafficking; ER Proteins
Applications Tested/Suitable for anti-ERp57 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-ERp57 antibody
WB: 1:1000
ICC/IF: 1:100

Immunocytochemistry (ICC) of anti-ERp57 antibody

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-ERp57 Monoclonal Antibody, Clone 4F9 . Tissue: HeLa Cells (Human Cervical Cancer). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-ERp57 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Endoplasmic reticulum, Melanosome. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ERp57 Antibody (D) Composite.
Mouse Anti-ERp57 Monoclonal Antibody – Cat# MBS808445

Western Blot (WB) of anti-ERp57 antibody

Western Blot analysis of Human Cervical Cancer cell line (HeLa) showing detection of 57 kDa Erp57 protein using Mouse Anti-Erp57 Monoclonal Antibody, Clone 4F9 . Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysate. Load: 15 ug. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Erp57 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 57 kDa.
Mouse Anti-ERp57 Monoclonal Antibody, Western Blot (WB) – Cat# MBS808445

Product References and Citations for anti-ERp57 antibody

1. Herbert D.N. and Molinari M. (2007) Physiol Rev. 87: 1377-1408. 2. Williams D.B. (2005) J Cell Sci. 119: 615-623 3. Maattanen P., et al. (2006) Biochem Cell Biol. 84: 881-889. 4. Oliver J.D., et al. (1999) Mol Bio Cell. 10: 2573-2582. 5. Oliver J.D., et al. (1997) Science 275: 86-88. 6. Solda T., et al. (2006) J Biol Chem 281: 6219-6226. 7. Kimura T., et al. (2005) Biochem Biophys Research Communications. 331 (1): 224-230. 8. Chen, G., et al. (2002) Clin Cancer Res 8(7): 2298-2305. 9. Tan, P., et al. F. (2002) J Immunol 168(4): 1950-1960.

Other Notes

Small volumes of anti-ERp57 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ERp57 purchase

MBS808445 is an antibody targeting ERp57. Also known as ERp57 Antibody, Clone 4F9: PerCP. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for ERp57. It may not necessarily be applicable to this product.

NCBI GI #
21361657
NCBI GeneID
2923
NCBI Accession #
NP_005304.3
UniProt Primary Accession #
P30101
UniProt Secondary Accession #
Q13453; Q14255; Q8IYF8; Q9UMU7
UniProt Related Accession #
P30101
GenBank Nucleotide #
NM_005313.4
NCBI Official Full Name
protein disulfide-isomerase A3
NCBI Official Synonym Full Names
protein disulfide isomerase family A member 3
NCBI Official Symbol
PDIA3
NCBI Official Synonym Symbols
P58; ER60; ERp57; ERp60; ERp61; GRP57; GRP58; PI-PLC; HsT17083; HEL-S-269; HEL-S-93n
UniProt Protein Name
Protein disulfide-isomerase A3
UniProt Synonym Protein Names
58 kDa glucose-regulated protein; 58 kDa microsomal protein; p58; Disulfide isomerase ER-60; Endoplasmic reticulum resident protein 57; ER protein 57; ERp57; Endoplasmic reticulum resident protein 60; ER protein 60; ERp60
UniProt Gene Name
PDIA3
UniProt Synonym Gene Names
ERP57; ERP60; GRP58; p58; ER protein 57; ERp57; ER protein 60; ERp60
NCBI Protein Information
protein disulfide-isomerase A3

NCBI Summary for ERp57

This gene encodes a protein of the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. The protein was once thought to be a phospholipase; however, it has been demonstrated that the protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and this protein mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates. This protein also functions as a molecular chaperone that prevents the formation of protein aggregates. [provided by RefSeq, Dec 2016]
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Research Articles on ERp57

1. ERp57 is involved in sperm capacitation and spermatozoa-zona pellucida binding.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ERp57 antibody

 Reference Product  PubMed Publications
 TAP1 antibody  >17 publications with ERp57 and TAP1
 HLA-B antibody  >10 publications with ERp57 and HLA-B
 CALR antibody  >7 publications with ERp57 and CALR
 HLA-A antibody  >5 publications with ERp57 and HLA-A
 HSPA5 antibody  >3 publications with ERp57 and HSPA5
 CANX antibody  >2 publications with ERp57 and CANX
 ERP27 antibody  >1 publications with ERp57 and ERP27
 TAPBP antibody  >1 publications with ERp57 and TAPBP

Pathways associated with anti-ERp57 antibody

 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Antigen Presentation: Folding, Assembly And Peptide Loading Of Class I MHC Pathway antibodies  Antigen Presentation: Folding, Assembly And Peptide Loading Of Class I MHC Pathway Diagram
 Antigen Processing And Presentation Pathway antibodies  Antigen Processing And Presentation Pathway Diagram
 Antigen Processing And Presentation Pathway antibodies  Antigen Processing And Presentation Pathway Diagram
 Antigen Processing-Cross Presentation Pathway antibodies  Antigen Processing-Cross Presentation Pathway Diagram
 Asparagine N-linked Glycosylation Pathway antibodies  Asparagine N-linked Glycosylation Pathway Diagram
 Calnexin/calreticulin Cycle Pathway antibodies  Calnexin/calreticulin Cycle Pathway Diagram
 Class I MHC Mediated Antigen Processing & Presentation Pathway antibodies  Class I MHC Mediated Antigen Processing & Presentation Pathway Diagram
 ER-Phagosome Pathway antibodies  ER-Phagosome Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram

Diseases associated with anti-ERp57 antibody

 Disease Name  Pubmed Publications
 Disease Models, Animal Antibodies  >23 publications with ERp57 and Disease Models, Animal
 Inflammation Antibodies  >20 publications with ERp57 and Inflammation
 Nervous System Diseases Antibodies  >19 publications with ERp57 and Nervous System Diseases
 Cardiovascular Diseases Antibodies  >15 publications with ERp57 and Cardiovascular Diseases
 Liver Diseases Antibodies  >15 publications with ERp57 and Liver Diseases
 Immune System Diseases Antibodies  >11 publications with ERp57 and Immune System Diseases
 Liver Neoplasms Antibodies  >10 publications with ERp57 and Liver Neoplasms
 Breast Neoplasms Antibodies  >10 publications with ERp57 and Breast Neoplasms
 Carcinoma, Hepatocellular Antibodies  >9 publications with ERp57 and Carcinoma, Hepatocellular
 Necrosis Antibodies  >8 publications with ERp57 and Necrosis

Organs/Tissues associated with anti-ERp57 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >47 publications with ERp57 and Blood
 Liver Antibodies  >29 publications with ERp57 and Liver
 Brain Antibodies  >22 publications with ERp57 and Brain
 Bone Antibodies  >20 publications with ERp57 and Bone
 Lung Antibodies  >17 publications with ERp57 and Lung
 Intestine Antibodies  >15 publications with ERp57 and Intestine
 Muscle Antibodies  >14 publications with ERp57 and Muscle
 Kidney Antibodies  >14 publications with ERp57 and Kidney
 Connective Tissue Antibodies  >11 publications with ERp57 and Connective Tissue
 Heart Antibodies  >10 publications with ERp57 and Heart
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