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4-Hydroxy-2-Hexenal (4-HHE) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS808693

MBS808693 is an antibody targeting 4-HHE. Also known as 4-Hydroxy-2-hexenal Antibody, Clone 6F10: Biotin. Reactive in Species Independent. Host: Mouse. More details

$660 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
4-Hydroxy-2-hexenal (4-HHE), Monoclonal Antibody
Full Product Name
4-Hydroxy-2-hexenal Antibody, Clone 6F10: Biotin
Product Gene Name
anti-4-HHE antibody
Product Synonym Names
Mouse Anti-4-Hydroxy-2-hexenal (4-HHE) Monoclonal IgG2b; 4-Hydroxy-2-hexenal Antibody: Biotin; 4-Hydroxy-2-hexenal (4-HHE) Antibody; 4-Hydroxy-2-hexenal Antibody; 4-HHE Antibody; MG Antibody; MG-modified protein Antibody; 4-Hydroxy-2-hexenal-modified; 4-Hydroxy-2-hexenal Antibody; 4-hydroxy Hexenal Antibody; HHE Antibody; 4-HHE Antibody
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
6F10
Host
Mouse
Species Reactivity
Species Independent
Purity/Purification
Protein G Purified
Concentration
1 mg/ml (lot specific)
Specificity
Specific for 4-Hydroxy-2-hexenal (4-HHE) and 4-Hydroxynonenal (4-HNE) modified proteins. Does not detect free 4-Hydroxy-2-hexenal (4-HHE) or 4-Hydroxynonenal (4-HNE). Does not cross-react with Acrolein, Crotonaldehyde, Hexanoyl Lysine, Malondialdehyde, or Methylglyoxal modified proteins.
Preparation and Storage
Store at -20 degree C.
Matching Pairs
Unconjugated Antibody: 4-Hydroxy-2-hexenal Clone #6F10 (MBS808682)
Biotin Conjugated Antibody: 4-Hydroxy-2-hexenal Clone #6F10 (MBS808693)
Matching Pairs
Unconjugated Antibody: N/A (MBS808693)
Biotin Conjugated Antibody: 4-Hydroxy-2-hexenal Clone #6F10 (MBS808693)
Target
4-Hydroxy-2-hexenal
Immunogen
Synthetic 4-Hydroxy-2-hexenal modified Keyhole Limpet Hemocyanin (KLH).
Conjugate
Biotin
Storage Buffer
PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide

Product Categories/Family for anti-4-HHE antibody

Antibodies; Cancer; Oxidative Stress; Lipid peroxidation
Applications Tested/Suitable for anti-4-HHE antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA (EIA), Flow Cytometry (FC/FACS)
Application Notes for anti-4-HHE antibody
WB: 1:1000
ICC/IF: 1:50
ELISA: 1:1000
FC/FACS: 1:50

Immunocytochemistry/Immunofluorescence (ICC/IF) of anti-4-HHE antibody

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-4-Hydroxy-2-hexenal Monoclonal Antibody, Clone 6F10. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-4-Hydroxy-2-hexenal Monoclonal Antibody at 1:400 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) 4-Hydroxy-2-hexenal Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.
Mouse Anti-4-HHE Monoclonal Antibody – Cat# MBS808693

Western Blot (WB) of anti-4-HHE antibody

Western Blot analysis of 4-hydroxy-2-hexanal-BSA Conjugate showing detection of 67 kDa 4-hydroxy-2-hexenal protein using Mouse Anti-4-hydroxy-2-hexenal Monoclonal Antibody, Clone 6F10. Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA (0.5 ug). Lane 3: 4-hydroxyl nonenal-BSA (0.5 ug). Lane 4: 4-hydroxy nonenal-BSA (2.0 ug). Lane 5: 4-hydroxy-2-hexenal (0.5 ug). Lane 6: 4-hydroxy-2-hexenal (2.0 ug). Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-4-hydroxy-2-hexenal Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.
Mouse Anti-4-HHE Monoclonal Antibody, Western Blot (WB) – Cat# MBS808693

Flow Cytometry (FC/FACS) of anti-4-HHE antibody

Flow Cytometry analysis using Mouse Anti-4-hydroxy-2-hexenal Monoclonal Antibody, Clone 6F10. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 90% Methanol. Primary Antibody: Mouse Anti-4-hydroxy-2-hexenal Monoclonal Antibody at 1:50 for 30 min on ice. Secondary Antibody: Goat Anti-Mouse: PE at 1:100 for 20 min at RT. Isotype Control: Non Specific IgG. Cells were subject to oxidative stress by treating with 250 uM H2O2 for 24 hours.
Mouse Anti-4-HHE Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS808693

Other Notes

Small volumes of anti-4-HHE antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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