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4-Hydroxynonenal (4-HNE) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS808701

Function: 4-Hydroxy-2-nonenol (4-HNE) is an unsaturated aldehyde derived from lipid peroxidation. 4-HNE is an electrophile and reacts with protein nucleophiles such as cysteine, histine, and lysine (1). Low levels of 4-HNE promote cell survival via cellular… More details

$665 / 0.1 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
4-Hydroxynonenal (4-HNE), Monoclonal Antibody
Full Product Name
4-Hydroxynonenal Antibody, Clone 12F7: ATTO 390
Product Gene Name
anti-4-HNE antibody
Product Synonym Names
Mouse Anti-4-Hydroxynonenal (4-HNE) Monoclonal IgG1; 4-Hydroxynonenal Antibody: ATTO 390; 4-Hydroxynonenal Antibody; 4-Hydroxy Nonenal (4-HNE) Antibody; 4-Hydroxy Nonenal Antibody; 4-HNE Antibody; 4-hydroxy Hexenal Antibody; HNE Antibody; 4-hydroxy-2-nonenal Antibody
Clonality
Monoclonal
Isotype
IgG1
Clone Number
12F7
Host
Mouse
Species Reactivity
All
Form/Format
10mM PBS / Bicarbonate
Purity/Purification
Protein G Purified
Concentration
1 mg/ml (lot specific)
Specificity
Specific for 4-Hydroxynonenal (4-HNE) modified proteins. Does not detect free 4-Hydroxynonenal. Does not cross-react with 4-Hydroxy-2-hexenal, Acrolein, Crotonaldehyde, Hexanoyl Lysine, Malondialdehyde, or Methylglyoxal modified proteins.
Preparation and Storage
Store at 4°C for one year. Avoid freeze / thaw cycles.
Matching Pairs
Unconjugated Antibody: 4-Hydroxynonenal Clone #12F7 (MBS808700)
ATTO 390 Conjugated Antibody: 4-Hydroxynonenal Clone #12F7 (MBS808701)
Matching Pairs
Unconjugated Antibody: N/A (MBS808701)
ATTO 390 Conjugated Antibody: 4-Hydroxynonenal Clone #12F7 (MBS808701)
Immunogen
Synthetic 4-Hydroxynonenal modified Keyhole Limpet Hemocyanin (KLH).
Properties
• High fluorescence yield
• Large Stokes-shift (89nm)
• Good photostability
• Moderately hydrophilic
• Good solubility in polar solvents
• Coumarin derivate, uncharged
Certificate of Analysis
A 1:1000 dilution of SMC-511 was sufficient for detection of 4-Hydroxynonenal in 0.5 µg of 4-Hydroxynonenal conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.

Product Categories/Family for anti-4-HNE antibody

Antibodies; Cancer; Oxidative Stress; Lipid peroxidation
Applications Tested/Suitable for anti-4-HNE antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA (EIA)
Application Notes for anti-4-HNE antibody
WB: 1:1000
ICC/IF: 1:50
ELISA: 1:1000

Optimal diltuions for assays should be determined by the user.

Immunocytochemistry/Immunofluorescence (ICC/IF) of anti-4-HNE antibody

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-4-Hydroxynonenal Monoclonal Antibody, Clone 12F7. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-4-Hydroxynonenal Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) 4-Hydroxynonenal Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.
Mouse Anti-4-HNE Monoclonal Antibody – Cat# MBS808701

Western Blot (WB) of anti-4-HNE antibody

Western Blot analysis of 4-hydroxy-nonenal-BSA Conjugate showing detection of 67 kDa 4-hydroxy-nonenal protein using Mouse Anti-4-hydroxy-nonenal Monoclonal Antibody, Clone 12F7. Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA (0.5 ug). Lane 3: 4-hydroxyl nonenal-BSA (0.5 ug). Lane 4: 4-hydroxy nonenal-BSA (2.0 ug). Lane 5: 4-hydroxy-2-hexenal (0.5 ug). Lane 6: 4-hydroxy-2-hexenal (2.0 ug). Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-4-hydroxy-nonenal Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.
Mouse Anti-4-HNE Monoclonal Antibody, Western Blot (WB) – Cat# MBS808701

Testing Data (TD) of anti-4-HNE antibody

Optical Properties
Mouse Anti-4-HNE Monoclonal Antibody, Testing Data (TD) – Cat# MBS808701

Testing Data (TD) of anti-4-HNE antibody

Chemical Structure
Mouse Anti-4-HNE Monoclonal Antibody, Testing Data (TD) – Cat# MBS808701

Other Notes

Small volumes of anti-4-HNE antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for 4-HNE purchase

MBS808701 is an antibody targeting 4-HNE. Also known as 4-Hydroxynonenal Antibody, Clone 12F7: ATTO 390. Reactive in All. Host: Mouse.
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