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7-Ketocholesterol (7-KC) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS808752

Lipid peroxidation occurs when oxidizing agents attack carbon-carbon double bonds found in unsaturated lipids. In addition to membrane degradation, oxidation end-products have been found to damage cell viability through their mutagenic and toxic properties.… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
7-Ketocholesterol (7-KC), Monoclonal Antibody
Full Product Name
7-Ketocholesterol Antibody, Clone 3F7: RPE
Product Gene Name
anti-7-KC antibody
Product Synonym Names
Mouse Anti-7-Ketocholesterol (7-KC) Monoclonal IgG3; 7-Ketocholesterol Antibody: RPE; 7-Ketocholesterol [7-KC] Antibody; 7-KC Antibody; 7-ketocholesterol (7-KC) Antibody; 7KC Antibody; 7KC-Modified Keyhole-Lympet Hemocyanine Antibody; 7KC-Modified KLH Antibody
Clonality
Monoclonal
Isotype
IgG3
Clone Number
3F7
Host
Mouse
Species Reactivity
ALL
Form/Format
PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide
Purity/Purification
Protein G Purified
Concentration
1 mg/ml (lot specific)
Specificity
Specific for 7-ketocholesterol modified proteins.
Preparation and Storage
Ships on blue ice. Store at -20°C; 1 year+ Avoid freeze/thaw cycle.
Matching Pairs
Unconjugated Antibody: 7-Ketocholesterol Clone #3F7 (MBS808736)
RPE Conjugated Antibody: 7-Ketocholesterol Clone #3F7 (MBS808752)
Matching Pairs
Unconjugated Antibody: N/A (MBS808752)
RPE Conjugated Antibody: 7-Ketocholesterol Clone #3F7 (MBS808752)
Immunogen
Synthetic 7-Ketocholesterol conjugated to Keyhole Limpet Hemocyanin (KLH).
Certificate of Analysis
A 1:1000 dilution of SMC-513 was sufficient for detection of 7-Ketocholesterol in 2 µg of 7-ketocholesterol conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.
Research Areas
Cancer, Lipid peroxidation, Oxidative Stress

Product Categories/Family for anti-7-KC antibody

Antibodies; Cancer; Oxidative Stress; Lipid peroxidation
Applications Tested/Suitable for anti-7-KC antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA (EIA)
Application Notes for anti-7-KC antibody
WB: 1:1000
ICC/IF: 1:50
ELISA: 1:1000

Optimal dilutions for assays should be determined by the user.

Immunocytochemistry/Immunofluorescence (ICC/IF) of anti-7-KC antibody

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-7-Ketocholesterol Monoclonal Antibody, Clone 3F7. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-7-Ketocholesterol Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) 7-Ketocholesterol Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.
Mouse Anti-7-KC Monoclonal Antibody – Cat# MBS808752

Western Blot (WB) of anti-7-KC antibody

Western Blot analysis of 7-Ketocholesterol-BSA Conjugate showing detection of 67 kDa 7-Ketocholesterol protein using Mouse Anti-7-Ketocholesterol Monoclonal Antibody, Clone 3F7. Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA (0.5 ug). Lane 3: BSA (2.0 ug). Lane 4: 7-ketocholesterol-BSA (0.5 ug). Lane 5: 7-ketocholesterol-BSA (2.0 ug). Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-7-Ketocholesterol Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.
Mouse Anti-7-KC Monoclonal Antibody, Western Blot (WB) – Cat# MBS808752

Other Notes

Small volumes of anti-7-KC antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for 7-KC purchase

MBS808752 is an antibody targeting 7-KC. Also known as 7-Ketocholesterol Antibody, Clone 3F7: RPE. Reactive in ALL. Host: Mouse.
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