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Methylglyoxal (MG) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS808806

MBS808806 is an antibody targeting MG. Also known as Methylglyoxal Antibody, Clone 9E7: RPE. Reactive in Species Independent. Host: Mouse. More details

$660 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Methylglyoxal (MG), Monoclonal Antibody
Full Product Name
Methylglyoxal Antibody, Clone 9E7: RPE
Product Gene Name
Product Synonym Names
Mouse Anti-Methylglyoxal (MG) Monoclonal IgG2a; Methylglyoxal Antibody: RPE; Methylglyoxal Antibody; 2-Oxopropanal Antibody; 2 oxo propanal Antibody; MG Antibody; Pyruvaldehyde Antibody; Methylglyoxal (MG) Antibody; MG-modified protein Antibody
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
9E7
Host
Mouse
Species Reactivity
Species Independent
Purity/Purification
Protein G Purified
Concentration
1 mg/ml (lot specific)
Specificity
Specific for Methylglyoxal modified proteins. Does not detect free Methylglyoxal. Does not cross-react with Acrolein, Hexanoyl Lysine, Malondialdeyhde, 4-Hydroxy-2-hexenal, 4-Hydroxy nonenal, or Crotonaldehyde modified proteins.
Preparation and Storage
Store at -20 degree C.
Matching Pairs
Unconjugated Antibody: Methylglyoxal Clone #9E7 (MBS808790)
RPE Conjugated Antibody: Methylglyoxal Clone #9E7 (MBS808806)
Matching Pairs
Unconjugated Antibody: N/A (MBS808806)
RPE Conjugated Antibody: Methylglyoxal Clone #9E7 (MBS808806)
Target
Methylglyoxal
Immunogen
Synthetic Methylglyoxal modified Keyhole Limpet Hemocyanin (KLH).
Conjugate
RPE
Storage Buffer
PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide
Applications Tested/Suitable for anti-MG antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA)
Application Notes for anti-MG antibody
WB: 1:1000
ICC/IF: 1:50
FC/FACS: 1:50
ELISA: 1:1000

Immunocytochemistry/Immunofluorescence (ICC/IF) of anti-MG antibody

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Methylglyoxal Monoclonal Antibody, Clone 9E7. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Methylglyoxal Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) Methylglyoxal Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.
Mouse Anti-MG Monoclonal Antibody – Cat# MBS808806

Western Blot (WB) of anti-MG antibody

Western Blot analysis of Methylglyoxal-BSA Conjugate showing detection of 67 kDa Methylglyoxal protein using Mouse Anti-Methylglyoxal Monoclonal Antibody, Clone 9E7. Lane 1: Molecular Weight Ladder (MW). Lane 2: Methylglyoxal-BSA. Lane 3: BSA. Load: 0.5 ug. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Methylglyoxal Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.
Mouse Anti-MG Monoclonal Antibody, Western Blot (WB) – Cat# MBS808806

Flow Cytometry (FC/FACS) of anti-MG antibody

Flow Cytometry analysis using Mouse Anti-Methylglyoxal Monoclonal Antibody, Clone 9E7. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 90% Methanol. Primary Antibody: Mouse Anti-Methylglyoxal Monoclonal Antibody at 1:50 for 30 min on ice. Secondary Antibody: Goat Anti-Mouse: PE at 1:100 for 20 min at RT. Isotype Control: Non Specific IgG. Cells were subject to oxidative stress by treating with 250 uM H2O2 for 24 hours.
Mouse Anti-MG Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS808806

Other Notes

Small volumes of anti-MG antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for MG. It may not necessarily be applicable to this product.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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