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Dityrosine (DT) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS808892

MBS808892 is an antibody targeting DT. Also known as Dityrosine Antibody, Clone 10A6: FITC. Reactive in Species Independent. Host: Mouse. More details

$650 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Dityrosine (DT), Monoclonal Antibody
Full Product Name
Dityrosine Antibody, Clone 10A6: FITC
Product Gene Name
anti-DT antibody
Product Synonym Names
Mouse Anti-Dityrosine (DT) Monoclonal IgG1; Dityrosine Antibody: FITC; Dityrosine Antibody; Dityrosine (DT) Antibody; DT Antibody; DY Antibody
Clonality
Monoclonal
Isotype
IgG1
Clone Number
10A6
Host
Mouse
Species Reactivity
Species Independent
Purity/Purification
Protein G Purified
Concentration
1 mg/ml (lot specific)
Specificity
Specific for dityrosine modified proteins. Does not cross-react with 3,5-dibromotyrosine or bromotyrosine modified proteins.
Preparation and Storage
Store at -20 degree C.
Matching Pairs
Unconjugated Antibody: Dityrosine Clone #10A6 (MBS808880)
FITC Conjugated Antibody: Dityrosine Clone #10A6 (MBS808892)
Matching Pairs
Unconjugated Antibody: N/A (MBS808892)
FITC Conjugated Antibody: Dityrosine Clone #10A6 (MBS808892)
Target
Dityrosine
Immunogen
Synthetic Dityrosine conjugated to Keyhole Limpet Hemocyanin (KLH).
Conjugate
FITC
Storage Buffer
PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide

Product Categories/Family for anti-DT antibody

Antibodies; Cancer; Oxidative Stress; Protein Oxidation; Cell Signaling; Post-translational Modifications; Oxidation
Applications Tested/Suitable for anti-DT antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA)
Application Notes for anti-DT antibody
WB: 1:1000
ICC/IF: 1:50
FC/FACS: 1:50
ELISA: 1:1000

Immunocytochemistry/Immunofluorescence (ICC/IF) of anti-DT antibody

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Dityrosine Monoclonal Antibody, Clone 10A6. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Dityrosine Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Localization: Cytoplasmic. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) Dityrosine Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.
Mouse Anti-DT Monoclonal Antibody – Cat# MBS808892

Western Blot (WB) of anti-DT antibody

Western Blot analysis of Dityrosine-BSA Conjugate showing detection of 67 kDa Dityrosine protein using Mouse Anti-Dityrosine Monoclonal Antibody, Clone 10A6. Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA. Lane 3: 3,5-Dibromotyrosine-BSA. Lane 4: Dityrosine-BSA. Lane 5: Bromotyrosine-BSA. Lane 6: 7-ketocholesterol-BSA. Load: 1 ug. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Dityrosine Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.
Mouse Anti-DT Monoclonal Antibody, Western Blot (WB) – Cat# MBS808892

Flow Cytometry (FC/FACS) of anti-DT antibody

Flow Cytometry analysis using Mouse Anti-Dityrosine Monoclonal Antibody, Clone 10A6. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 90% Methanol. Primary Antibody: Mouse Anti-Dityrosine Monoclonal Antibody at 1:50 for 30 min on ice. Secondary Antibody: Goat Anti-Mouse: PE at 1:100 for 20 min at RT. Isotype Control: Non Specific IgG. Cells were subject to oxidative stress by treating with 250 uM H2O2 for 24 hours.
Mouse Anti-DT Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS808892

Western Blot (WB) of anti-DT antibody

Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of Dityrosine protein using Mouse Anti-Dityrosine Monoclonal Antibody, Clone 10A6. Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysate. Lane 3: H2O2 treated HeLa cell lysate. Load: 12 ug. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Dityrosine Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT.
Mouse Anti-DT Monoclonal Antibody, Western Blot (WB) – Cat# MBS808892

Other Notes

Small volumes of anti-DT antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for DT. It may not necessarily be applicable to this product.

Protein Family
Dityrosine transporter

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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