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KDEL Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS809098

The endoplasmic reticulum is part of a protein sorting pathway, or in essence, the transportation system of the eukaryotic cell. The majority of endoplasmic reticulum resident proteins are retained in the endoplasmic reticulum through a retention motif. This… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
KDEL, Monoclonal Antibody
Full Product Name
KDEL Antibody, Clone 2D6: ATTO 488
Product Gene Name
Product Synonym Names
Mouse Anti-KDEL Monoclonal IgG1; KDEL Antibody: ATTO 488; KDEL Antibody; Lys Asp Glu Leu Antibody; Lys-Asp-Glu-Leu (KDEL) Antibody; Lys-Asp-Glu-Leu Antibody; Lysine-aspartic acid-glutamate-leucine Antibody
Clonality
Monoclonal
Isotype
IgG1
Clone Number
2D6
Host
Mouse
Species Reactivity
Human; Mouse; Rat
Purity/Purification
Protein G Purified
Concentration
1 mg/ml (lot specific)
Specificity
Detects KDEL tagged proteins.
Preparation and Storage
Store at -20 degree C.
Matching Pairs
Unconjugated Antibody: KDEL Clone #2D6 (MBS809096)
ATTO 488 Conjugated Antibody: KDEL Clone #2D6 (MBS809098)
Matching Pairs
Unconjugated Antibody: N/A (MBS809098)
ATTO 488 Conjugated Antibody: KDEL Clone #2D6 (MBS809098)
Target
KDEL
Immunogen
KDEL peptide (C-AVKDEL-COOH) conjugated at the N-terminal to Bovine Serum Albumin (BSA)
Conjugate
ATTO 488
Storage Buffer
PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide
Applications Tested/Suitable for anti-KDEL antibody
Western Blot (WB)
Application Notes for anti-KDEL antibody
WB: 1:1000

Western Blot (WB) of anti-KDEL antibody

Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of Multiple KDEL protein using Mouse Anti-KDEL Monoclonal Antibody, Clone 2D6. Lane 1: Molecular Weight Ladder (MW). Lane 2: Anti-KDEL. Lane 3: Anti-KDEL control (SPC-109). Lane 4: Anti-PDI control (SPC-114). Lane 5: Anti-Calreticulin control (SPC-122). Lane 6: Anti-GRP78 control (SPC-107). Lane 7: Anti-GRP94 control (SPC-101). Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-KDEL Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG:HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: Multiple.
Mouse Anti-KDEL Monoclonal Antibody – Cat# MBS809098

Product References and Citations for anti-KDEL antibody

1. Ozawa K., et al. (2008) Mol Pharmacol. 74:1610. 2. Austin R.C., et al. (2003) J Biol Chem. 278: 17438.

Other Notes

Small volumes of anti-KDEL antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for KDEL purchase

MBS809098 is an antibody targeting KDEL. Also known as KDEL Antibody, Clone 2D6: ATTO 488. Reactive in Human; Mouse; Rat. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for KDEL. It may not necessarily be applicable to this product.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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