Mouse Anti-c-FOS Monoclonal Antibody – Cat# MBS809215

Western Blot analysis of Human Serum starved HeLa cells treated with PMA showing detection of 41 kDa c-fos protein using Mouse Anti-c-fos Monoclonal Antibody, Clone 12G11. Lane 1: Molecular Weight Ladder (MW). Lane 2: Serum starved HeLa cell lysates. Lane 3: Serum starved HeLa cells treated with PMA (Phorbol 12-myristate 13-acetate). Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-c-fos Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG:HRP at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 41 kDa. Other Band(s): 62 kDa. Observed molecular weight of band is higher than predicted 41 kDa band due to post translational modifications.

c-FOS Antibody – Mouse Monoclonal

Name: c-FOS Antibody; Monoclonal; Mouse; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS809215
Price: 540 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS809215

MBS809215 is an antibody targeting c-Fos. Also known as c-Fos Antibody, Clone 12G11: Biotin. Reactive in Human. Host: Mouse. More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

c-Fos, Monoclonal Antibody

Full Product Name

c-Fos Antibody, Clone 12G11: Biotin

Product Gene Name

anti-c-Fos antibody

Product Synonym Names

Mouse Anti-Human c-fos Monoclonal IgG2a; c-Fos Antibody: Biotin; c-fos Antibody; Activator protein 1 antibody; AP 1 antibody; C FOS antibody; Cellular oncogene c fos antibody; Cellular oncogene fos antibody; FBJ murine osteosarcoma viral (v fos) oncogene homolog (oncogene FOS) antibody; FBJ murine osteosarcoma viral oncogene homolog antibody; FBJ murine osteosarcoma viral v fos oncogene homolog antibody; FBJ Osteosarcoma Virus antibody; FOS antibody; FOS protein antibody; FOS_HUMAN antibody; G0 G1 switch regulatory protein 7 antibody; G0/G1 switch regulatory protein 7 antibody; G0S7 antibody; Oncogene FOS antibody; p55 antibody; proto oncogene c Fos antibody; Proto oncogene protein c fos antibody; Proto-oncogene c-Fos antibody; v fos FBJ murine osteosarcoma viral oncogene homolog antibody

Clonality

Monoclonal

Isotype

IgG2a

Clone Number

12G11

Host

Mouse

Species Reactivity

Human

Purity/Purification

Protein G Purified

Concentration

1 mg/ml (lot specific)

Specificity

Detects 62 kDa band. Observed molecular weight of band is higher than predicted 41 kDa band due to post translational modifications.

Preparation and Storage

Store at -20 degree C.

Molecular Weight

36,301 Da

OMIM

164810

3D Structure

ModBase 3D Structure for P01100

Target

c-fos

Immunogen

Synthetic peptide from the N-terminal of Human c-Fos (aa. 280-380)

Immunogen Species

Human

Cellular Localization

Cytosol; Endoplasmic reticulum; Nucleus

Conjugate

Biotin

Storage Buffer

PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide

Tissue Specificity

Expressed at very low levels in quiescent cells. When cells are stimulated to reenter growth, they undergo 2 waves of expression, the first one peaks 7.5 minutes following FBS induction. At this stage, the protein is localized endoplasmic reticulum. The second wave of expression occurs at about 20 minutes after induction and peaks at 1 hour. At this stage, the protein becomes nuclear.

Product Categories/Family for anti-c-Fos antibody

Antibodies; Neuroscience; Cell Markers; Neuron Markers; Cytoplasmic Markers; Cell Signaling; Epigenetics and Nuclear Signaling; Transcription; Domain Families; Cancer; Tumor Biomarkers; Oncoproteins; Cell Signaling; Epigenetics and Nuclear Signaling; Chro

Applications Tested/Suitable for anti-c-Fos antibody

Western Blot (WB)

Application Notes for anti-c-Fos antibody

WB: 1:1000

Other Notes

Small volumes of anti-c-Fos antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for c-Fos. It may not necessarily be applicable to this product.

NCBI GI #

4885241

NCBI GeneID

2353

NCBI Accession #

NP_005243.1

UniProt Primary Accession #

P01100

UniProt Secondary Accession #

P18849; A8K4E2; B4DQ65

UniProt Related Accession #

P01100

GenBank Nucleotide #

NM_005252.3

NCBI Official Full Name

proto-oncogene c-Fos

NCBI Official Synonym Full Names

Fos proto-oncogene, AP-1 transcription factor subunit

NCBI Official Symbol

FOS

NCBI Official Synonym Symbols

p55; AP-1; C-FOS

UniProt Protein Name

Proto-oncogene c-Fos

UniProt Synonym Protein Names

Cellular oncogene fos; G0/G1 switch regulatory protein 7

Protein Family

Transcription factor

UniProt Gene Name

FOS

UniProt Synonym Gene Names

G0S7

NCBI Protein Information

proto-oncogene c-Fos

NCBI Summary for c-Fos

The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the FOS gene has also been associated with apoptotic cell death. [provided by RefSeq, Jul 2008]

UniProt Comments for c-Fos

Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. On TGF-beta activation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD-binding site to regulate TGF-beta-mediated signaling. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation. In growing cells, activates phospholipid synthesis, possibly by activating CDS1 and PI4K2A. This activity requires Tyr-dephosphorylation and association with the endoplasmic reticulum.

Research Articles on c-Fos

1. our results strongly suggest a novel role of c-Fos as a regulator of epithelial-mesenchymal transition and cancer stem cell(CSC) reprogramming in Head and neck squamous cell carcinoma (HNSCC)cells, which may hold potential as a CSC-directed therapeutic approach to improve HNSCC treatment

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-c-Fos antibody

Reference Product	PubMed Publications
JUN antibody	>8726 publications with c-Fos and JUN
JUNB antibody	>741 publications with c-Fos and JUNB
JUND antibody	>529 publications with c-Fos and JUND
ATF2 antibody	>90 publications with c-Fos and ATF2
CREB1 antibody	>72 publications with c-Fos and CREB1
MAPK8 antibody	>52 publications with c-Fos and MAPK8
MAPK1 antibody	>34 publications with c-Fos and MAPK1
IL2 antibody	>27 publications with c-Fos and IL2
MAPK3 antibody	>7 publications with c-Fos and MAPK3
MAPK9 antibody	>2 publications with c-Fos and MAPK9

Pathways associated with anti-c-Fos antibody

Products by Pathway	Pathway Diagram
ATF-2 Transcription Factor Network Pathway antibodies	ATF-2 Transcription Factor Network Pathway Diagram
Activated TLR4 Signalling Pathway antibodies	Activated TLR4 Signalling Pathway Diagram
Activation Of The AP-1 Family Of Transcription Factors Pathway antibodies	Activation Of The AP-1 Family Of Transcription Factors Pathway Diagram
Amphetamine Addiction Pathway antibodies	Amphetamine Addiction Pathway Diagram
Amphetamine Addiction Pathway antibodies	Amphetamine Addiction Pathway Diagram
Apoptosis Pathway antibodies	Apoptosis Pathway Diagram
Apoptosis Pathway antibodies	Apoptosis Pathway Diagram
Apoptosis Modulation And Signaling Pathway antibodies	Apoptosis Modulation And Signaling Pathway Diagram
B Cell Receptor Signaling Pathway antibodies	B Cell Receptor Signaling Pathway Diagram
B Cell Receptor Signaling Pathway antibodies	B Cell Receptor Signaling Pathway Diagram

Diseases associated with anti-c-Fos antibody

Disease Name	Pubmed Publications
Nervous System Diseases Antibodies	>2709 publications with c-Fos and Nervous System Diseases
Disease Models, Animal Antibodies	>1898 publications with c-Fos and Disease Models, Animal
Pain Antibodies	>1707 publications with c-Fos and Pain
Inflammation Antibodies	>1273 publications with c-Fos and Inflammation
Necrosis Antibodies	>857 publications with c-Fos and Necrosis
Cell Transformation, Neoplastic Antibodies	>726 publications with c-Fos and Cell Transformation, Neoplastic
Seizures Antibodies	>578 publications with c-Fos and Seizures
Hyperalgesia Antibodies	>505 publications with c-Fos and Hyperalgesia
Breast Neoplasms Antibodies	>433 publications with c-Fos and Breast Neoplasms
Hypertension Antibodies	>417 publications with c-Fos and Hypertension

Organs/Tissues associated with anti-c-Fos antibody

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>12571 publications with c-Fos and Brain
Blood Antibodies	>4046 publications with c-Fos and Blood
Nerve Antibodies	>2861 publications with c-Fos and Nerve
Spinal Cord Antibodies	>1627 publications with c-Fos and Spinal Cord
Muscle Antibodies	>1586 publications with c-Fos and Muscle
Bone Antibodies	>1521 publications with c-Fos and Bone
Vascular Antibodies	>1283 publications with c-Fos and Vascular
Liver Antibodies	>1091 publications with c-Fos and Liver
Heart Antibodies	>1064 publications with c-Fos and Heart
Skin Antibodies	>785 publications with c-Fos and Skin