• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

GABARAPL2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS809494

MBS809494 is an antibody targeting GABARAPL2. Also known as GABARAPL2 Antibody: ATTO 594. Reactive in Human; Rat. Host: Rabbit. More details

DISCONTINUED
Click here to view our similar product(s) OR click here to contact our technical support team for suitable replacement.
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Product Name
GABARAPL2, Polyclonal Antibody
Full Product Name
GABARAPL2 Antibody: ATTO 594
Product Gene Name
Product Synonym Names
Rabbit Anti-Human GABARAPL2 Polyclonal; ATG8 Antibody; FLC3A Antibody; General protein transport factor p16 Antibody; GEF-2 Antibody; GABARAPL2 Antibody; GBRL2_HUMAN Antibody; GEF2 Antibody; GABA(A) receptor-associated protein-like 2 Antibody; MAP1 light chain 3-related protein Antibody; Golgi-associated ATPase enhancer of 16 kDa Antibody; Gabarapl2 Antibody; GATE-16 Antibody; Gamma-aminobutyric acid receptor-associated protein-like 2 Antibody; Ganglioside expression factor 2 Antibody; ATG8C Antibody; GATE16 Antibody
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human; Rat
Purity/Purification
Peptide Affinity Purified
Concentration
1 mg/ml (lot specific)
Specificity
Detects ~14 kDa.
Preparation and Storage
Store at -20 degree C.
Molecular Weight
13,667 Da
OMIM
607452
3D Structure
ModBase 3D Structure for P60520
Target
GABARAPL2
Immunogen
Synthetic peptide from the C-terminal of Human GABARAPL2
Immunogen Species
Human
Cellular Localization
Golgi Apparatus; Cytoplasmic Vesicle; Autophagosome
Conjugate
ATTO 594
Storage Buffer
PBS, 50% glycerol, 0.09% sodium azide
Tissue Specificity
Ubiquitous. Expressed at high levels in the brain, heart, prostate, ovary, spleen and skeletal muscle. Expressed at very low levels in lung, thymus and small intestine.
Applications Tested/Suitable for anti-GABARAPL2 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-GABARAPL2 antibody
WB: 1:1000
ICC/IF: 1:100

Immunocytochemistry (ICC) of anti-GABARAPL2 antibody

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-GABARAPL2 Polyclonal Antibody . Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-GABARAPL2 Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasmic Vesicle, Autophagosome . Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) GABARAPL2 Antibody. (D) Composite.
Rabbit Anti-GABARAPL2 Polyclonal Antibody – Cat# MBS809494

Western Blot (WB) of anti-GABARAPL2 antibody

Western blot analysis of Rat Liver cell lysates showing detection of ~14 kDa GABARAPL2 protein using Rabbit Anti-GABARAPL2 Polyclonal Antibody . Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Liver cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-GABARAPL2 Polyclonal Antibody at 1:1000 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~14 kDa.
Rabbit Anti-GABARAPL2 Polyclonal Antibody, Western Blot (WB) – Cat# MBS809494

Other Notes

Small volumes of anti-GABARAPL2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for GABARAPL2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
gamma-aminobutyric acid receptor-associated protein-like 2
NCBI Official Synonym Full Names
GABA type A receptor associated protein like 2
NCBI Official Symbol
GABARAPL2
NCBI Official Synonym Symbols
ATG8; GEF2; ATG8C; GEF-2; GATE16; GATE-16
UniProt Protein Name
Gamma-aminobutyric acid receptor-associated protein-like 2
UniProt Synonym Protein Names
GABA(A) receptor-associated protein-like 2; Ganglioside expression factor 2; GEF-2; General protein transport factor p16; Golgi-associated ATPase enhancer of 16 kDa; GATE-16; MAP1 light chain 3-related protein
UniProt Gene Name
GABARAPL2
UniProt Synonym Gene Names
FLC3A; GEF2; GEF-2; GATE-16
NCBI Protein Information
gamma-aminobutyric acid receptor-associated protein-like 2

UniProt Comments for GABARAPL2

Ubiquitin-like modifier involved in intra-Golgi traffic. Modulates intra-Golgi transport through coupling between NSF activity and SNAREs activation. It first stimulates the ATPase activity of NSF which in turn stimulates the association with GOSR1 (). Involved in autophagy. Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation.
Show more

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000