• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

ATG4B Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS809563

Autophagy related 4B, cysteine peptidase (ATG4B or APG4B) is a C-54 cysteine protease that also belongs to the family of autophagin poteins. Specifically it cleaves LC3, and mutations can be associated with strong inhibition of autophagosome formation. More details

DISCONTINUED
Click here to view our similar product(s) OR click here to contact our technical support team for suitable replacement.
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Product Name
ATG4B, Polyclonal Antibody
Full Product Name
ATG4B Antibody: ATTO 390
Product Gene Name
Product Synonym Names
Rabbit Anti-Human ATG4B Polyclonal; MGC1353 Antibody; Autophagy-related cysteine endopeptidase 1 Antibody; ATG4B Antibody; hAPG4B Antibody; AUT-like 1 cysteine endopeptidase Antibody; Cysteine protease ATG4B Antibody; Autophagy-related protein 4 homolog B Antibody; Autophagin-1 Antibody; EC:3.4.22.- Antibody; ATG4B_HUMAN Antibody; APG4B Antibody; APG4 autophagy 4 homolog B Antibody; AUTL1 Antibody; KIAA0943 Antibody; Autophagy related cysteine endopeptidase 1 Antibody; Autophagin 1 Antibody; ATG4 autophagy related 4 homolog B (S. cerevisiae) Antibody; AUT like 1 cysteine endopeptidase Antibody; Autophagy related protein 4 homolog B Antibody
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human; Mouse
Purity/Purification
Peptide Affinity Purified
Concentration
1 mg/ml (lot specific)
Specificity
Detects ~44 kDa.
Preparation and Storage
Store at -20 degree C.
Matching Pairs
Unconjugated Antibody: ATG4B (MBS809562)
ATTO 390 Conjugated Antibody: ATG4B (MBS809563)
Matching Pairs
Unconjugated Antibody: N/A (MBS809563)
ATTO 390 Conjugated Antibody: ATG4B (MBS809563)
Molecular Weight
42,622 Da
OMIM
611338
3D Structure
ModBase 3D Structure for Q9Y4P1
Target
ATG4B
Immunogen
Synthetic peptide from the N-terminal to mid of Human ATG4B (aa. 94-106)
Immunogen Species
Human
Cellular Localization
Cytoplasm
Conjugate
ATTO 390
Storage Buffer
PBS, 50% glycerol, 0.09% sodium azide
Tissue Specificity
Mainly expressed in the skeletal muscle, followed by brain, heart, liver and pancreas.
Applications Tested/Suitable for anti-ATG4B antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-ATG4B antibody
WB: 1:1000
ICC/IF: 1:100

Western Blot (WB) of anti-ATG4B antibody

Western blot analysis of Mouse Brain cell lysates showing detection of ATG4B protein using Rabbit Anti-ATG4B Polyclonal Antibody (SPC-630). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Brain cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-ATG4B Polyclonal Antibody (SPC-630) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Other Band(s): Band appears at ~40 kDa.
Rabbit Anti-ATG4B Polyclonal Antibody – Cat# MBS809563

Product References and Citations for anti-ATG4B antibody

1. Kabeya Y., et al. (2004) J Cell Sci. 117: 2805-2812. 2. Tanida I., et al. (2004) J Biol Chem. 279: 36268-36276. 3. Hemelaar J., et al. (2003) J Biol Chem. 278: 51841-51850.

Other Notes

Small volumes of anti-ATG4B antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ATG4B purchase

MBS809563 is an antibody targeting ATG4B. Also known as ATG4B Antibody: ATTO 390. Reactive in Human; Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for ATG4B. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
cysteine protease ATG4B isoform a
NCBI Official Synonym Full Names
autophagy related 4B cysteine peptidase
NCBI Official Symbol
ATG4B
NCBI Official Synonym Symbols
APG4B; AUTL1
UniProt Protein Name
Cysteine protease ATG4B
UniProt Synonym Protein Names
AUT-like 1 cysteine endopeptidase; Autophagin-1; Autophagy-related cysteine endopeptidase 1; Autophagy-related protein 4 homolog B; hAPG4B
Protein Family
UniProt Gene Name
ATG4B
UniProt Synonym Gene Names
APG4B; AUTL1; KIAA0943; hAPG4B
NCBI Protein Information
cysteine protease ATG4B

NCBI Summary for ATG4B

Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
Show more

UniProt Comments for ATG4B

Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3, GABARAPL1, GABARAPL2 and GABARAP, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms.
Show more

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000