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ATG4D Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS809620

ATG4D, Auotphagy related 4D, cysteine peptidases, belong to the autophagy-related protein 4 family of C54 endopeptidases. Members of this family play a role in the biogenesis of autophagosomes (1). Cysteine protease ATG4D: Cysteine protease required for the… More details

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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Product Name
ATG4D, Polyclonal Antibody
Full Product Name
ATG4D Antibody: ATTO 594
Product Gene Name
Product Synonym Names
Rabbit Anti-Human ATG4D Polyclonal; Autophagin-4 Antibody; APG4D Antibody; ATG4D Antibody; Cysteine protease ATG4D Antibody; AUTL4 Antibody; Autophagy-related cysteine endopeptidase 4 Antibody; EC:3.4.22.- Antibody; AUT-like 4 cysteine endopeptidase Antibody; Autophagy-related protein 4 homolog D Antibody
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Purity/Purification
Peptide Affinity Purified
Concentration
1 mg/ml (lot specific)
Specificity
Detects ~53 kDa.
Preparation and Storage
Store at -20 degree C.
Molecular Weight
16,008 Da
OMIM
611340
3D Structure
ModBase 3D Structure for Q86TL0
Target
ATG4D
Immunogen
Synthetic peptide from the N-terminal of Human ATG4D (aa. 98-108)
Immunogen Species
Human
Cellular Localization
Cytoplasm
Conjugate
ATTO 594
Storage Buffer
PBS, 50% glycerol, 0.09% sodium azide
Tissue Specificity
Mainly expressed in skeletal muscle and, to a lower extent, in testis.
Applications Tested/Suitable for anti-ATG4D antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-ATG4D antibody
WB: 1:1000
ICC/IF: 1:100

Western Blot (WB) of anti-ATG4D antibody

Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of 52.9 kDa ATG4D protein using Rabbit Anti-ATG4D Polyclonal Antibody (SPC-635). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human HeLa cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-ATG4D Polyclonal Antibody (SPC-635) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 52.9 kDa. Other Band(s): ~45 and 48 kDa.
Rabbit Anti-ATG4D Polyclonal Antibody – Cat# MBS809620

Product References and Citations for anti-ATG4D antibody

1. "Entrez Gene: Autophagy related 4D, cysteine peptidase". 2. Li M., et al. (2011) J Biol Chem. 286: 7327-7338.

Other Notes

Small volumes of anti-ATG4D antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ATG4D purchase

MBS809620 is an antibody targeting ATG4D. Also known as ATG4D Antibody: ATTO 594. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for ATG4D. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
cysteine protease ATG4D isoform 2
NCBI Official Synonym Full Names
autophagy related 4D cysteine peptidase
NCBI Official Symbol
ATG4D
NCBI Official Synonym Symbols
APG4D; AUTL4; APG4-D
UniProt Protein Name
Cysteine protease ATG4D
UniProt Synonym Protein Names
AUT-like 4 cysteine endopeptidase; Autophagin-4; Autophagy-related cysteine endopeptidase 4; Autophagy-related protein 4 homolog D
Protein Family
UniProt Gene Name
ATG4D
UniProt Synonym Gene Names
APG4D; AUTL4
NCBI Protein Information
cysteine protease ATG4D

NCBI Summary for ATG4D

Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene belongs to the autophagy-related protein 4 (Atg4) family of C54 endopeptidases. Members of this family encode proteins that play a role in the biogenesis of autophagosomes, which sequester the cytosol and organelles for degradation by lysosomes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
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UniProt Comments for ATG4D

Cysteine protease ATG4D: Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3 and GABARAPL2, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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