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ULK3 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS809728

UNC-51 like kinase 3, ULK3, is a serine-threonine protein kinase that acts as a regulator of SHH signaling and autophagy. Is phosphorylates in vitro GLI2. More details

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Product Overview

Product Name
ULK3, Polyclonal Antibody
Full Product Name
ULK3 Antibody: ATTO 594
Product Gene Name
Product Synonym Names
Rabbit Anti-Human ULK3 Polyclonal; Serine/threonine-protein kinase ULK3 Antibody; EC:2.7.11.1 Antibody; Ulk3 Antibody; DKFZP434C131 Antibody; Unc-51-like kinase 3 Antibody; FLJ90566 Antibody; ULK3_HUMAN Antibody; unc 51 like kinase 3 (C. elegans) Antibody; ULK3 Antibody
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human; Mouse; Rat
Purity/Purification
Peptide Affinity Purified
Concentration
1 mg/ml (lot specific)
Specificity
Detects ~53 kDa.
Preparation and Storage
Store at -20 degree C.
Molecular Weight
54,997 Da
OMIM
613472
3D Structure
ModBase 3D Structure for Q6PHR2
Target
ULK3
Immunogen
Synthetic peptide from the N-terminal of Human ULK3 (aa. 166-177)
Immunogen Species
Human
Cellular Localization
Cytoplasm
Conjugate
ATTO 594
Storage Buffer
PBS, 50% glycerol, 0.09% sodium azide
Tissue Specificity
Widely expressed. Highest levels observed in fetal brain. In adult tissues, high levels in brain, liver and kidney, moderate levels in testis and adrenal gland and low levels in heart, lung, stomach, thymus, prostate and placenta. In the brain, highest expression in the hippocampus, high levels also detected in the cerebellum, olfactory bulb and optic nerve. In the central nervous system, lowest levels in the spinal cord.
Applications Tested/Suitable for anti-ULK3 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-ULK3 antibody
WB: 1:1000
ICC/IF: 1:100

Western Blot (WB) of anti-ULK3 antibody

Western blot analysis of Human Lung carcinoma epithelial cell line (A549) lysate showing detection of 53.4 kDa ULK3 protein using Rabbit Anti-ULK3 Polyclonal Antibody (SPC-642). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human A549 cell lysates. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-ULK3 Polyclonal Antibody (SPC-642) at 1:1000 for 16 hours at 4 degree C. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 53.4 kDa. Other Band(s): ~25 kDa in all lysates.
Rabbit Anti-ULK3 Polyclonal Antibody – Cat# MBS809728

Western Blot (WB) of anti-ULK3 antibody

Western blot analysis of Rat Brain cell lysates showing detection of 53.4 kDa ULK3 protein using Rabbit Anti-ULK3 Polyclonal Antibody (SPC-642). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-ULK3 Polyclonal Antibody (SPC-642) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 53.4 kDa.
Rabbit Anti-ULK3 Polyclonal Antibody, Western Blot (WB) – Cat# MBS809728

Product References and Citations for anti-ULK3 antibody

1. Malovergan A., et al. (2010) J Biol Chem. 285(39): 30079-30090. 2. Young A.R., et al. (2009) Genes Dev. 23(7):798-803.

Other Notes

Small volumes of anti-ULK3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ULK3 purchase

MBS809728 is an antibody targeting ULK3. Also known as ULK3 Antibody: ATTO 594. Reactive in Human; Mouse; Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for ULK3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
serine/threonine-protein kinase ULK3 isoform a
NCBI Official Synonym Full Names
unc-51 like kinase 3
NCBI Official Symbol
ULK3
UniProt Protein Name
Serine/threonine-protein kinase ULK3
UniProt Synonym Protein Names
Unc-51-like kinase 3
UniProt Gene Name
ULK3
NCBI Protein Information
serine/threonine-protein kinase ULK3

UniProt Comments for ULK3

Serine/threonine protein kinase that acts as a regulator of Sonic hedgehog (SHH) signaling and autophagy. Acts as a negative regulator of SHH signaling in the absence of SHH ligand: interacts with SUFU, thereby inactivating the protein kinase activity and preventing phosphorylation of GLI proteins (GLI1, GLI2 and/or GLI3). Positively regulates SHH signaling in the presence of SHH: dissociates from SUFU, autophosphorylates and mediates phosphorylation of GLI2, activating it and promoting its nuclear translocation. Phosphorylates in vitro GLI2, as well as GLI1 and GLI3, although less efficiently. Also acts as a regulator of autophagy: following cellular senescence, able to induce autophagy.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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