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WIPI2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS809791

MBS809791 is an antibody targeting WIPI2. Also known as WIPI2 Antibody: HRP. Reactive in Human. Host: Rabbit. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
WIPI2, Polyclonal Antibody
Full Product Name
WIPI2 Antibody: HRP
Product Gene Name
Product Synonym Names
Rabbit Anti-Human WIPI2 Polyclonal; WIPI2 Antibody; WD repeat domain phosphoinositide-interacting protein 2 Antibody; WIPI-2 Antibody; WIPI49-like protein 2 Antibody; CGI-50 Antibody; ATG21 Antibody; WIPI 2 Antibody; ATG18B Antibody
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Purity/Purification
Peptide Affinity Purified
Concentration
1 mg/ml (lot specific)
Specificity
Detects ~55 kDa.
Preparation and Storage
Store at -20 degree C.
Molecular Weight
48,249 Da
OMIM
609225
3D Structure
ModBase 3D Structure for Q9Y4P8
Target
WIPI2
Immunogen
Synthetic peptide from the C-terminal of Human WIPI2
Immunogen Species
Human
Cellular Localization
Preautophagosomal Structure Membrane; Peripheral Membrane Protein; Cytoplasmic Side
Conjugate
HRP
Storage Buffer
PBS, 50% glycerol, 0.09% sodium azide
Tissue Specificity
Ubiquitously expressed (at protein level). Highly expressed in heart, skeletal muscle and pancreas. Expression is down-regulated in pancreatic and in kidney tumors.

Product Categories/Family for anti-WIPI2 antibody

Applications Tested/Suitable for anti-WIPI2 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-WIPI2 antibody
WB: 1:1000
ICC/IF: 1:100

Immunocytochemistry (ICC) of anti-WIPI2 antibody

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-WIPI2 Polyclonal Antibody . Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-WIPI2 Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm . Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) WIPI2 Antibody (D) Composite.
Rabbit Anti-WIPI2 Polyclonal Antibody – Cat# MBS809791

Immunohistochemistry (IHC) of anti-WIPI2 antibody

Immunohistochemistry analysis using Rabbit Anti-WIPI2 Polyclonal Antibody . Tissue: Heart. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-WIPI2 Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 20X. HRP-DAB Detection.
Rabbit Anti-WIPI2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS809791

Western Blot (WB) of anti-WIPI2 antibody

Western blot analysis of Human HeLa and HEK293Trap cell lysates showing detection of ~49.4 kDa WIPI2 protein using Rabbit Anti-WIPI2 Polyclonal Antibody . Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysates. Lane 3: 293Trap cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-WIPI2 Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~49.4 kDa.
Rabbit Anti-WIPI2 Polyclonal Antibody, Western Blot (WB) – Cat# MBS809791

Other Notes

Small volumes of anti-WIPI2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for WIPI2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
WD repeat domain phosphoinositide-interacting protein 2 isoform c
NCBI Official Synonym Full Names
WD repeat domain, phosphoinositide interacting 2
NCBI Official Symbol
WIPI2
NCBI Official Synonym Symbols
Atg21; ATG18B; CGI-50; WIPI-2
UniProt Protein Name
WD repeat domain phosphoinositide-interacting protein 2
UniProt Synonym Protein Names
WIPI49-like protein 2
UniProt Gene Name
WIPI2
UniProt Synonym Gene Names
WIPI-2
NCBI Protein Information
WD repeat domain phosphoinositide-interacting protein 2

NCBI Summary for WIPI2

WD40 repeat proteins are key components of many essential biologic functions. They regulate the assembly of multiprotein complexes by presenting a beta-propeller platform for simultaneous and reversible protein-protein interactions. Members of the WIPI subfamily of WD40 repeat proteins, such as WIPI2, have a 7-bladed propeller structure and contain a conserved motif for interaction with phospholipids (Proikas-Cezanne et al., 2004 [PubMed 15602573]).[supplied by OMIM, Mar 2008]
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UniProt Comments for WIPI2

Early component of the autophagy machinery being involved in formation of preautophagosomal structures and their maturation into mature phagosomes in response to phosphatidylinositol 3-phosphate (PtdIns3P). May bind PtdIns3P.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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