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WDR45 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS809811

WIPI-4 is a 360 amino acid protein containing three WD repeats. Existing as three alternatively spliced isoforms, WIPI-4 is ubiquitously expressed but found at highest levels in heart and skeletal muscle. WD repeat proteins are involved in a variety of… More details

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Product Overview

Online Datasheet in PDF
Product Name
WDR45, Polyclonal Antibody
Full Product Name
WDR45 Antibody: PerCP
Product Gene Name
Product Synonym Names
Rabbit Anti-Human WDR45 Polyclonal; WIPI4 Antibody; WDR45 Antibody; WDRX1 Antibody; WD repeat domain phosphoinositide-interacting protein 4 Antibody; WIPI-4 Antibody; WDRXI4 Antibody; WD repeat-containing protein 45 Antibody; JM5 Antibody
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human; Rat
Purity/Purification
Peptide Affinity Purified
Concentration
1 mg/ml (lot specific)
Specificity
Detects ~40 kDa.
Preparation and Storage
Store at -20 degree C.
Matching Pairs
Unconjugated Antibody: WDR45 (MBS809796)
PerCP Conjugated Antibody: WDR45 (MBS809811)
Matching Pairs
Unconjugated Antibody: N/A (MBS809811)
PerCP Conjugated Antibody: WDR45 (MBS809811)
Molecular Weight
39,939 Da
OMIM
300526
3D Structure
ModBase 3D Structure for Q9Y484
Target
WDR45
Immunogen
Synthetic peptide from the C-terminal of Human WIPI4 (aa. 312-322)
Immunogen Species
Human
Conjugate
PerCP
Storage Buffer
PBS, 50% glycerol, 0.09% sodium azide
Tissue Specificity
Ubiquitously expressed, with high expression in skeletal muscle and heart. Weakly expressed in liver and placenta. Expression is down-regulated in pancreatic and in kidney tumors.
Applications Tested/Suitable for anti-WDR45 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-WDR45 antibody
WB: 1:1000
ICC/IF: 1:100

Western Blot (WB) of anti-WDR45 antibody

Western blot analysis of Human Embryonic kidney epithelial cell line (HEK293T) lysate showing detection of 40 kDa WDR45 protein using Rabbit Anti-WDR45 Polyclonal Antibody (SPC-651). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human 293Trap cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-WDR45 Polyclonal Antibody (SPC-651) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 40 kDa.
Rabbit Anti-WDR45 Polyclonal Antibody – Cat# MBS809811

Product References and Citations for anti-WDR45 antibody

1. http://www.genecards.org/cgi-bin/carddisp.pl?gene=WDR45 2. Strausberg R.L., et al. (2002) Proc Natl Acad Sci U S A. 99(26): 16899-16903. 3. Haack T.B., et al. (2012) Am J Hum Genet. 91(6): 1144-1149.

Other Notes

Small volumes of anti-WDR45 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for WDR45 purchase

MBS809811 is an antibody targeting WDR45. Also known as WDR45 Antibody: PerCP. Reactive in Human; Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for WDR45. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
WD repeat domain phosphoinositide-interacting protein 4 isoform 2
NCBI Official Synonym Full Names
WD repeat domain 45
NCBI Official Symbol
WDR45
NCBI Official Synonym Symbols
JM5; NBIA4; NBIA5; WDRX1; WIPI4; WIPI-4
UniProt Protein Name
WD repeat domain phosphoinositide-interacting protein 4
UniProt Synonym Protein Names
WD repeat-containing protein 45
UniProt Gene Name
WDR45
UniProt Synonym Gene Names
WDRX1; WDRXI4; WIPI4; WIPI-4
NCBI Protein Information
WD repeat domain phosphoinositide-interacting protein 4

NCBI Summary for WDR45

This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. This gene has a pseudogene at chromosome 4q31.3. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene, but the biological validity and full-length nature of some variants have not been determined. [provided by RefSeq, Jul 2008]
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UniProt Comments for WDR45

Plays an important role in the autophagy pathway, which is the major intracellular degradation system by which cytoplasmic materials are packaged into autophagosomes and delivered to lysosomes for degradation.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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