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BCL-2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS809814

MBS809814 is an antibody targeting Bcl-2. Also known as Bcl-2 Antibody. Reactive in Human; Rat. Host: Rabbit. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
Bcl-2, Polyclonal Antibody
Full Product Name
Bcl-2 Antibody
Product Gene Name
Product Synonym Names
Rabbit Anti-Human Bcl-2 Polyclonal; Protein phosphatase 1 regulatory subunit 50 Antibody; AW986256 Antibody; BCL2_HUMAN Antibody; Bcl2 Antibody; Apoptosis regulator Bcl2 Antibody; BCL2 Antibody; B cell leukemia/lymphoma 2 Antibody; Leukemia/lymphoma; B-cell; 2 Antibody; B cell CLL/lymphoma 2 Antibody; Bcl-2 Antibody; Oncogene B-cell leukemia 2 Antibody; D830018M01Rik Antibody; PPP1R50 Antibody; D630044D05Rik Antibody; Apoptosis regulator Bcl-2 Antibody; C430015F12Rik Antibody
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human; Rat
Purity/Purification
Peptide Affinity Purified
Concentration
1 mg/ml (lot specific)
Specificity
Detects ~26 kDa.
Preparation and Storage
Store at -20 degree C.
Molecular Weight
22,337 Da
OMIM
151430
3D Structure
ModBase 3D Structure for P10415
Target
Bcl-2
Immunogen
Synthetic peptide from the mid-protein of Human Bcl-2
Immunogen Species
Human
Cellular Localization
Mitochondrion Outer Membrane; Single-Pass Membrane Protein; Nucleus Membrane; Single-Pass Membrane Protein; Endoplasmic Reticulum Membrane; Single-Pass Membrane Protein
Conjugate
Unconjugated
Storage Buffer
PBS, 50% glycerol, 0.09% sodium azide
Tissue Specificity
Expressed in a variety of tissues.
Applications Tested/Suitable for anti-Bcl-2 antibody
Western Blot (WB)
Application Notes for anti-Bcl-2 antibody
WB: 1:1000

Immunohistochemistry (IHC) of anti-Bcl-2 antibody

Immunohistochemistry analysis using Rabbit Anti-Bcl-2 Polyclonal Antibody . Tissue: Colon. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-Bcl-2 Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.
Rabbit Anti-BCL-2 Polyclonal Antibody – Cat# MBS809814

Immunohistochemistry (IHC) of anti-Bcl-2 antibody

Immunohistochemistry analysis using Rabbit Anti-Bcl-2 Polyclonal Antibody . Tissue: Colon. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-Bcl-2 Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.
Rabbit Anti-BCL-2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS809814

Western Blot (WB) of anti-Bcl-2 antibody

Western blot analysis of Human Embryonic kidney epithelial cell line (HEK293T) lysate showing detection of ~26.3 kDa Bcl-2 protein using Rabbit Anti-Bcl-2 Polyclonal Antibody . Lane 1: Molecular Weight Ladder (MW). Lane 2: 293Trap cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Bcl-2 Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~26.3 kDa.
Rabbit Anti-BCL-2 Polyclonal Antibody, Western Blot (WB) – Cat# MBS809814

Other Notes

Small volumes of anti-Bcl-2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for Bcl-2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
apoptosis regulator Bcl-2 alpha isoform
NCBI Official Synonym Full Names
BCL2, apoptosis regulator
NCBI Official Symbol
BCL2
NCBI Official Synonym Symbols
Bcl-2; PPP1R50
UniProt Protein Name
Apoptosis regulator Bcl-2
UniProt Gene Name
BCL2
NCBI Protein Information
apoptosis regulator Bcl-2

NCBI Summary for Bcl-2

This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]
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UniProt Comments for Bcl-2

Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (PubMed:17418785).
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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