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PIK3R4 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS809850

MBS809850 is an antibody targeting PIK3R4. Also known as PIK3R4 Antibody. Reactive in Human; Rat. Host: Rabbit. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
PIK3R4, Polyclonal Antibody
Full Product Name
PIK3R4 Antibody
Product Gene Name
Product Synonym Names
Rabbit Anti-Human PIK3R4 Polyclonal; VPS15 Antibody; Phosphoinositide 3-kinase regulatory subunit 4 Antibody; PI3-kinase regulatory subunit 4 Antibody; PI3-kinase p150 subunit Antibody; PIK3R4 Antibody; Phosphoinositide 3-kinase adaptor protein Antibody
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human; Rat
Purity/Purification
Peptide Affinity Purified
Concentration
1 mg/ml (lot specific)
Specificity
Detects ~160 kDa.
Preparation and Storage
Store at -20 degree C.
Molecular Weight
153,103 Da
OMIM
602610
3D Structure
ModBase 3D Structure for Q99570
Target
PIK3R4
Immunogen
Synthetic peptide from the C-terminal of Human PIK3R4 (aa. 1314-1325)
Immunogen Species
Human
Cellular Localization
Late Endosome; Cytoplasmic Vesicle; Autophagosome; Membrane; Lipid-Anchor
Conjugate
Unconjugated
Storage Buffer
PBS, 50% glycerol, 0.09% sodium azide
Tissue Specificity
Ubiquitously expressed.
Applications Tested/Suitable for anti-PIK3R4 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-PIK3R4 antibody
WB: 1:1000
ICC/IF: 1:100

Western Blot (WB) of anti-PIK3R4 antibody

Western blot analysis of Rat Brain cell lysates showing detection of 153.1 kDa PIK3R4 protein using Rabbit Anti-PIK3R4 Polyclonal Antibody (SPC-660). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-PIK3R4 Polyclonal Antibody (SPC-660) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 153.1 kDa.
Rabbit Anti-PIK3R4 Polyclonal Antibody – Cat# MBS809850

Other Notes

Small volumes of anti-PIK3R4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PIK3R4. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
phosphoinositide 3-kinase regulatory subunit 4
NCBI Official Synonym Full Names
phosphoinositide-3-kinase regulatory subunit 4
NCBI Official Symbol
PIK3R4
NCBI Official Synonym Symbols
p150; VPS15
UniProt Protein Name
Phosphoinositide 3-kinase regulatory subunit 4
UniProt Synonym Protein Names
PI3-kinase p150 subunit; Phosphoinositide 3-kinase adaptor protein
UniProt Gene Name
PIK3R4
UniProt Synonym Gene Names
; PI3-kinase regulatory subunit 4
NCBI Protein Information
phosphoinositide 3-kinase regulatory subunit 4

UniProt Comments for PIK3R4

Regulatory subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and cytokinesis, probably in the context of PI3KC3-C2 (PubMed:20643123).
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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