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Pi 3 Kinase Class 3 (PIK3C3) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS809877

MBS809877 is an antibody targeting PIK3C3. Also known as PI 3 Kinase Class 3 antibody: Alkaline Phosphatase. Reactive in Mouse; Rat. Host: Rabbit. More details

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Testing Data View PDF
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Product Overview

Product Name
PI 3 Kinase Class 3 (PIK3C3), Polyclonal Antibody
Full Product Name
PI 3 Kinase Class 3 antibody: Alkaline Phosphatase
Product Gene Name
Product Synonym Names
Rabbit Anti-Human PI 3 Kinase Class 3 Polyclonal; PI3 kinase type 3 Antibody; PI 3 kinase type 3 Antibody; hVps34 Antibody; Phosphatidylinositol 3-kinase p100 subunit Antibody; Phosphatidylinositol 3 kinase p100 subunit Antibody; PI3K type 3 Antibody; PI3-kinase type 3 Antibody; EC:2.7.1.137 Antibody; PK3C3_HUMAN Antibody; PtdIns-3-kinase type 3 Antibody; PIK3C3 Antibody; VPS34 Antibody; Pik3c3 Antibody; Phosphoinositide 3 kinase class 3 Antibody; Phosphatidylinositol 3-kinase catalytic subunit type 3 Antibody; PtdIns 3 kinase type 3 Antibody; Phosphoinositide-3-kinase class 3 Antibody; Phosphatidylinositol 3 kinase class 3 Antibody; Vps 34 Antibody; Phosphatidylinositol 3 kinase catalytic subunit type 3 Antibody; MGC61518 Antibody; Vps34 Antibody; PI3 kinase class 3 Antibody
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Mouse; Rat
Purity/Purification
Peptide Affinity Purified
Concentration
1 mg/ml (lot specific)
Specificity
Detects ~101.5 kDa.
Preparation and Storage
Store at -20 degree C.
Molecular Weight
101,549 Da
OMIM
602609
3D Structure
ModBase 3D Structure for Q8NEB9
Target
PI 3 Kinase Class 3
Immunogen
Synthetic peptide from the N-terminal of Human PI 3 Kinase Class 3
Immunogen Species
Human
Cellular Localization
Midbody; Late Endosome; Cytoplasmic Vesicle; Autophagosome
Conjugate
Alkaline Phosphatase
Storage Buffer
PBS, 50% glycerol, 0.09% sodium azide
Tissue Specificity
Ubiquitously expressed, with a highest expression in skeletal muscle.
Applications Tested/Suitable for anti-PIK3C3 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-PIK3C3 antibody
WB: 1:1000
ICC/IF: 1:100

Immunocytochemistry (ICC) of anti-PIK3C3 antibody

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-PI 3 Kinase Class 3 Polyclonal Antibody . Tissue: C2C12 Cells (Mouse Myoblast cell line). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-PI 3 Kinase Class 3 Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Midbody, Late Endosome, Cytoplasmic Vesicle, Autophagosome . Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) PI 3 Kinase Class 3 antibody (D) Composite.
Rabbit Anti-PIK3C3 Polyclonal Antibody – Cat# MBS809877

Immunohistochemistry (IHC) of anti-PIK3C3 antibody

Immunohistochemistry analysis using Rabbit Anti-PI 3 Kinase Class 3 Polyclonal Antibody . Tissue: Bladder. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-PI 3 Kinase Class 3 Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.
Rabbit Anti-PIK3C3 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS809877

Western Blot (WB) of anti-PIK3C3 antibody

Western blot analysis of Rat, Mouse Liver cell lysates showing detection of ~101.5 kDa PI 3 Kinase Class 3 protein using Rabbit Anti-PI 3 Kinase Class 3 Polyclonal Antibody . Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Liver cell lysates. Lane 3: Mouse Liver cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-PI 3 Kinase Class 3 Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~101.5 kDa.
Rabbit Anti-PIK3C3 Polyclonal Antibody, Western Blot (WB) – Cat# MBS809877

Other Notes

Small volumes of anti-PIK3C3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PIK3C3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
phosphatidylinositol 3-kinase catalytic subunit type 3 isoform 1
NCBI Official Synonym Full Names
phosphatidylinositol 3-kinase catalytic subunit type 3
NCBI Official Symbol
PIK3C3
NCBI Official Synonym Symbols
VPS34; Vps34; hVps34
UniProt Protein Name
Phosphatidylinositol 3-kinase catalytic subunit type 3
UniProt Synonym Protein Names
Phosphatidylinositol 3-kinase p100 subunit; Phosphoinositide-3-kinase class 3; hVps34
UniProt Gene Name
PIK3C3
UniProt Synonym Gene Names
PI3-kinase type 3; PI3K type 3; PtdIns-3-kinase type 3
NCBI Protein Information
phosphatidylinositol 3-kinase catalytic subunit type 3

UniProt Comments for PIK3C3

Catalytic subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and required for the abcission step in cytokinesis, probably in the context of PI3KC3-C2 (PubMed:20643123, PubMed:20208530). Involved in the transport of lysosomal enzyme precursors to lysosomes. Required for transport from early to late endosomes ().
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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