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RIP3 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS809960

MBS809960 is an antibody targeting RIP3. Also known as RIP3 Antibody: ATTO 488. Reactive in Human; Mouse. Host: Rabbit. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
RIP3, Polyclonal Antibody
Full Product Name
RIP3 Antibody: ATTO 488
Product Gene Name
Product Synonym Names
Rabbit Anti-Human RIP3 Polyclonal; RIPK3 Antibody; Receptor-interacting protein 3 Antibody; Receptor interacting protein 3 Antibody; Receptor interacting serine/threonine protein kinase 3 Antibody; RIP-3 Antibody; RIPK3_HUMAN Antibody; Receptor-interacting serine/threonine-protein kinase 3 Antibody; RIPK 3 Antibody; RIP 3 Antibody; Receptor interacting serine threonine kinase 3 Antibody; RIP3 Antibody; RIP like protein kinase 3 Antibody; RIP-like protein kinase 3 Antibody
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human; Mouse
Purity/Purification
Peptide Affinity Purified
Concentration
1 mg/ml (lot specific)
Specificity
Detects ~57 kDa.
Preparation and Storage
Store at -20 degree C.
Molecular Weight
25,326 Da
OMIM
605817
3D Structure
ModBase 3D Structure for Q9Y572
Target
RIP3
Immunogen
Synthetic peptide from the N-terminal to middle of Human RIP3
Immunogen Species
Human
Cellular Localization
Cytoplasm; Cytosol; Cell Membrane; Mitochondrion
Conjugate
ATTO 488
Storage Buffer
PBS, 50% glycerol, 0.09% sodium azide
Tissue Specificity
Highly expressed in the pancreas. Detected at lower levels in heart, placenta, lung and kidney. Isoform 3 is significantly increased in colon and lung cancers.
Applications Tested/Suitable for anti-RIP3 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-RIP3 antibody
WB: 1:1000
ICC/IF: 1:100

Immunocytochemistry (ICC) of anti-RIP3 antibody

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-RIP3 Polyclonal Antibody . Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-RIP3 Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) RIP3 Antibody. (D) Composite.
Rabbit Anti-RIP3 Polyclonal Antibody – Cat# MBS809960

Immunohistochemistry (IHC) of anti-RIP3 antibody

Immunohistochemistry analysis using Rabbit Anti-RIP3 Polyclonal Antibody . Tissue: Colon Cancer. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-RIP3 Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.
Rabbit Anti-RIP3 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS809960

Western Blot (WB) of anti-RIP3 antibody

Western blot analysis of Mouse Brain cell lysates showing detection of ~56.9 kDa RIP3 protein using Rabbit Anti-RIP3 Polyclonal Antibody . Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Brain cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-RIP3 Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~56.9 kDa.
Rabbit Anti-RIP3 Polyclonal Antibody, Western Blot (WB) – Cat# MBS809960

Other Notes

Small volumes of anti-RIP3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for RIP3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
receptor-interacting serine/threonine-protein kinase 3
NCBI Official Synonym Full Names
receptor interacting serine/threonine kinase 3
NCBI Official Symbol
RIPK3
NCBI Official Synonym Symbols
RIP3
UniProt Protein Name
Receptor-interacting serine/threonine-protein kinase 3
UniProt Synonym Protein Names
RIP-like protein kinase 3; Receptor-interacting protein 3; RIP-3
UniProt Gene Name
RIPK3
UniProt Synonym Gene Names
RIP3; RIP-3
NCBI Protein Information
receptor-interacting serine/threonine-protein kinase 3

NCBI Summary for RIP3

The product of this gene is a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases, and contains a C-terminal domain unique from other RIP family members. The encoded protein is predominantly localized to the cytoplasm, and can undergo nucleocytoplasmic shuttling dependent on novel nuclear localization and export signals. It is a component of the tumor necrosis factor (TNF) receptor-I signaling complex, and can induce apoptosis and weakly activate the NF-kappaB transcription factor. [provided by RefSeq, Jul 2008]
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UniProt Comments for RIP3

Essential for necroptosis, a programmed cell death process in response to death-inducing TNF-alpha family members. Upon induction of necrosis, RIPK3 interacts with, and phosphorylates RIPK1 and MLKL to form a necrosis-inducing complex. RIPK3 binds to and enhances the activity of three metabolic enzymes: GLUL, GLUD1, and PYGL. These metabolic enzymes may eventually stimulate the tricarboxylic acid cycle and oxidative phosphorylation, which could result in enhanced ROS production.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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