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OTUB2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS820185

MBS820185 is an antibody targeting OTUB2. Reactive in Human, Mouse, Rat, Bovine. Host: Rabbit. Rabbit polyclonal antibody to OTUB2 More details

$225 / 0.03 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
OTUB2, Polyclonal Antibody
Full Product Name
Anti-OTUB2 Antibody
Product Gene Name
Product Synonym Names
C14orf137; OTB2; OTU2; Ubiquitin thioesterase OTUB2; Deubiquitinating enzyme OTUB2; OTU domain-containing ubiquitin aldehyde-binding protein 2; Otubain-2; Ubiquitin-specific-processing protease OTUB2
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat, Bovine
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Specificity
Recognizes endogenous levels of OTUB2 protein.
Preparation and Storage
Upon receipt aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
Antibody/Peptide Pairs
OTUB2 peptide (MBS823612) is used for blocking the activity of OTUB2 antibody (MBS820185)
Molecular Weight
8,637 Da
OMIM
608338
3D Structure
ModBase 3D Structure for Q96DC9
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human OTUB2. The exact sequence is proprietary.
Applications Tested/Suitable for anti-OTUB2 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-OTUB2 antibody
WB (1/500 - 1/1000), IHC (1/50 - 1/100)

Western Blot (WB) of anti-OTUB2 antibody

Western blot analysis of OTUB2 expression in HeLa (A), HepG2 (B) whole cell lysates.
Rabbit Anti-OTUB2 Polyclonal Antibody – Cat# MBS820185

Western Blot (WB) of anti-OTUB2 antibody

Western blot analysis of OTUB2 expression in HepG2 (A),SGC7901 (B), AML12 (C), H9C2 (D) whole cell lysates.
Rabbit Anti-OTUB2 Polyclonal Antibody, Western Blot (WB) – Cat# MBS820185

Immunohistochemistry (IHC) of anti-OTUB2 antibody

Immunohistochemical analysis of OTUB2 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Rabbit Anti-OTUB2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS820185

Other Notes

Small volumes of anti-OTUB2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for OTUB2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
ubiquitin thioesterase OTUB2
NCBI Official Synonym Full Names
OTU deubiquitinase, ubiquitin aldehyde binding 2
NCBI Official Symbol
OTUB2
NCBI Official Synonym Symbols
OTB2; OTU2; C14orf137
UniProt Protein Name
Ubiquitin thioesterase OTUB2
UniProt Synonym Protein Names
Deubiquitinating enzyme OTUB2; OTU domain-containing ubiquitin aldehyde-binding protein 2; Otubain-2; Ubiquitin-specific-processing protease OTUB2
UniProt Gene Name
OTUB2
UniProt Synonym Gene Names
C14orf137; OTB2; OTU2
UniProt Entry Name
OTUB2_HUMAN
NCBI Protein Information
ubiquitin thioesterase OTUB2; otubain-2; deubiquitinating enzyme OTUB2; ubiquitin-specific protease otubain 2; OTU domain, ubiquitin aldehyde binding 2; ubiquitin-specific-processing protease OTUB2; OTU domain-containing ubiquitin aldehyde-binding protein 2

NCBI Summary for OTUB2

This gene encodes one of several deubiquitylating enzymes. Ubiquitin modification of proteins is needed for their stability and function; to reverse the process, deubiquityling enzymes remove ubiquitin. This protein contains an OTU domain and binds Ubal (ubiquitin aldehyde); an active cysteine protease site is present in the OTU domain. [provided by RefSeq, Aug 2011]
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UniProt Comments for OTUB2

OTUB2: Hydrolase that can remove conjugated ubiquitin from proteins in vitro and may therefore play an important regulatory role at the level of protein turnover by preventing degradation. Mediates deubiquitination of both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains, with a preference for 'Lys-63'-linked polyubiquitin chains. Belongs to the peptidase C65 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Hydrolase; EC 3.4.19.12; Ubiquitin-specific protease

Chromosomal Location of Human Ortholog: 14q32.12

Cellular Component: nucleus

Molecular Function: ubiquitin-specific protease activity

Biological Process: protein deubiquitination; proteolysis
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-OTUB2 antibody

Organs/Tissues associated with anti-OTUB2 antibody

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