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AKT2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS820210

MBS820210 is an antibody targeting AKT2. Reactive in Human, Mouse, Rat, Chicken, Dog. Host: Rabbit. Rabbit polyclonal antibody to AKT2 More details

$225 / 0.03 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
AKT2, Polyclonal Antibody
Full Product Name
Anti-AKT2 Antibody
Product Gene Name
anti-AKT2 antibody
Product Synonym Names
RAC-beta serine/threonine-protein kinase; Protein kinase Akt-2; Protein kinase B beta; PKB beta; RAC-PK-beta
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat, Chicken, Dog
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Specificity
Recognizes endogenous levels of AKT2 protein.
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
Antibody/Peptide Pairs
AKT2 peptide (MBS823943) is used for blocking the activity of AKT2 antibody (MBS820210)
Molecular Weight
51,083 Da
OMIM
gene 240900
3D Structure
ModBase 3D Structure for P31751
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human AKT2. The exact sequence is proprietary.
Applications Tested/Suitable for anti-AKT2 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
Application Notes for anti-AKT2 antibody
WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/ICC (1/100 - 1/500)

Western Blot (WB) of anti-AKT2 antibody

Western blot analysis of AKT2 expression in HeLa (A), HepG2 (B), NIH3T3 (C), mouse brain (D), rat brain (E) whole cell lysates.
Rabbit Anti-AKT2 Polyclonal Antibody – Cat# MBS820210

Immunohistochemistry (IHC) of anti-AKT2 antibody

Immunohistochemical analysis of AKT2 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Rabbit Anti-AKT2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS820210

Immunofluorescence (IF) of anti-AKT2 antibody

Immunofluorescent analysis of AKT2 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Rabbit Anti-AKT2 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS820210

Other Notes

Small volumes of anti-AKT2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for AKT2. It may not necessarily be applicable to this product.

NCBI GI #
4502023
NCBI GeneID
208
NCBI Accession #
NP_001617.1
UniProt Primary Accession #
P31751
UniProt Secondary Accession #
Q05BV0; Q0VAN0; Q0VAN1; Q68GC0; B2RBD8
UniProt Related Accession #
P31751
GenBank Nucleotide #
NM_001626.5
NCBI Official Full Name
RAC-beta serine/threonine-protein kinase isoform 1
NCBI Official Synonym Full Names
v-akt murine thymoma viral oncogene homolog 2
NCBI Official Symbol
AKT2
NCBI Official Synonym Symbols
PKBB; PRKBB; HIHGHH; PKBBETA; RAC-BETA
UniProt Protein Name
RAC-beta serine/threonine-protein kinase
UniProt Synonym Protein Names
Protein kinase Akt-2; Protein kinase B beta; PKB beta; RAC-PK-beta
Protein Family
Potassium channel
UniProt Gene Name
AKT2
UniProt Synonym Gene Names
PKB beta
UniProt Entry Name
AKT2_HUMAN
NCBI Protein Information
RAC-beta serine/threonine-protein kinase; PKB beta; RAC-PK-beta; protein kinase Akt-2; protein kinase B beta; rac protein kinase beta; murine thymoma viral (v-akt) homolog-2

NCBI Summary for AKT2

This gene is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. The gene was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. The encoded protein is a general protein kinase capable of phophorylating several known proteins. [provided by RefSeq, Jul 2008]
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UniProt Comments for AKT2

Akt2: an AGC kinase. Plays critical roles in glucose metabolism and the development or maintenance of proper adipose tissue and islet mass for which other Akt/PKB isoforms are unable to fully compensate. Amplified and overexpressed in human ovarian carcinoma cell lines and amplified in some primary ovarian and pancreatic tumors. Antisense blocks invasiveness in xenografts. Expressed in several insulin-responsive tissues, and one case of Type II diabetes has been associated with a likely LOF point mutation. Mouse mutants have defects in insulin response.

Protein type: Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.1; Oncoprotein; Protein kinase, AGC; AGC group; AKT family

Chromosomal Location of Human Ortholog: 19q13.1-q13.2

Cellular Component: nucleoplasm; plasma membrane; cell cortex; cytosol; nucleus

Molecular Function: protein serine/threonine kinase activity; protein binding; kinase activity; ATP binding

Biological Process: fat cell differentiation; glycogen biosynthetic process; apoptosis; carbohydrate transport; positive regulation of glycogen biosynthetic process; intracellular protein transport across a membrane; myelin maintenance in the peripheral nervous system; protein modification process; glucose metabolic process; positive regulation of vesicle fusion; signal transduction; regulation of cell migration; protein amino acid phosphorylation; positive regulation of fatty acid beta-oxidation; regulation of translation; positive regulation of glucose import; cellular response to insulin stimulus; insulin receptor signaling pathway; positive regulation of protein amino acid phosphorylation

Disease: Hypoinsulinemic Hypoglycemia With Hemihypertrophy; Diabetes Mellitus, Noninsulin-dependent
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Research Articles on AKT2

1. Results show that miR-302b inhibits hepatocellular carcinoma cell proliferation and growth in vitro and in vivo by targeting AKT2.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-AKT2 antibody

 Reference Product  PubMed Publications
 MTOR antibody  >91 publications with AKT2 and MTOR
 FOXO1 antibody  >28 publications with AKT2 and FOXO1
 TSC2 antibody  >7 publications with AKT2 and TSC2
 INS antibody  >3 publications with AKT2 and INS
 GSK3B antibody  >2 publications with AKT2 and GSK3B
 AKT1S1 antibody  >2 publications with AKT2 and AKT1S1
 CHUK antibody  >1 publications with AKT2 and CHUK
 TCL1A antibody  >1 publications with AKT2 and TCL1A

Pathways associated with anti-AKT2 antibody

 Products by Pathway  Pathway Diagram
 AKT Phosphorylates Targets In The Cytosol Pathway antibodies  AKT Phosphorylates Targets In The Cytosol Pathway Diagram
 AKT Phosphorylates Targets In The Nucleus Pathway antibodies  AKT Phosphorylates Targets In The Nucleus Pathway Diagram
 AKT-mediated Inactivation Of FOXO1A Pathway antibodies  AKT-mediated Inactivation Of FOXO1A Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 Activation Of BAD And Translocation To Mitochondria Pathway antibodies  Activation Of BAD And Translocation To Mitochondria Pathway Diagram
 Activation Of BH3-only Proteins Pathway antibodies  Activation Of BH3-only Proteins Pathway Diagram
 Activation Of PKB Pathway antibodies  Activation Of PKB Pathway Diagram
 Acute Myeloid Leukemia Pathway antibodies  Acute Myeloid Leukemia Pathway Diagram

Diseases associated with anti-AKT2 antibody

 Disease Name  Pubmed Publications
 Breast Neoplasms Antibodies  >78 publications with AKT2 and Breast Neoplasms
 Adenocarcinoma Antibodies  >69 publications with AKT2 and Adenocarcinoma
 Ovarian Neoplasms Antibodies  >66 publications with AKT2 and Ovarian Neoplasms
 Liver Diseases Antibodies  >39 publications with AKT2 and Liver Diseases
 Neoplasms, Experimental Antibodies  >34 publications with AKT2 and Neoplasms, Experimental
 Nervous System Diseases Antibodies  >33 publications with AKT2 and Nervous System Diseases
 Cardiovascular Diseases Antibodies  >31 publications with AKT2 and Cardiovascular Diseases
 Liver Neoplasms Antibodies  >29 publications with AKT2 and Liver Neoplasms
 Brain Diseases Antibodies  >26 publications with AKT2 and Brain Diseases
 Hyperglycemia Antibodies  >25 publications with AKT2 and Hyperglycemia

Organs/Tissues associated with anti-AKT2 antibody

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >184 publications with AKT2 and Muscle
 Blood Antibodies  >125 publications with AKT2 and Blood
 Liver Antibodies  >95 publications with AKT2 and Liver
 Lung Antibodies  >66 publications with AKT2 and Lung
 Vascular Antibodies  >57 publications with AKT2 and Vascular
 Heart Antibodies  >46 publications with AKT2 and Heart
 Adipose Tissue Antibodies  >43 publications with AKT2 and Adipose Tissue
 Brain Antibodies  >43 publications with AKT2 and Brain
 Bone Antibodies  >36 publications with AKT2 and Bone
 Connective Tissue Antibodies  >29 publications with AKT2 and Connective Tissue
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