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IRS1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS820213

MBS820213 is an antibody targeting IRS1. Reactive in Human, Mouse, Rat, Pig. Host: Rabbit. Rabbit polyclonal antibody to IRS1 More details

$225 / 0.03 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
IRS1, Polyclonal Antibody
Full Product Name
Anti-IRS1 Antibody
Product Gene Name
anti-IRS1 antibody
Product Synonym Names
Insulin receptor substrate 1; IRS-1
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat, Pig
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Specificity
Recognizes endogenous levels of IRS1 protein.
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
Antibody/Peptide Pairs
IRS1 peptide (MBS824372) is used for blocking the activity of IRS1 antibody (MBS820213)
Molecular Weight
131,591 Da
OMIM
phenotype 147545
3D Structure
ModBase 3D Structure for P35568
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human IRS1. The exact sequence is proprietary.
Applications Tested/Suitable for anti-IRS1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
Application Notes for anti-IRS1 antibody
WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/ICC (1/100 - 1/500)

Western Blot (WB) of anti-IRS1 antibody

Western blot analysis of IRS1 expression in HeLa (A), HEK293 (B) whole cell lysates.
Rabbit Anti-IRS1 Polyclonal Antibody – Cat# MBS820213

Immunohistochemistry (IHC) of anti-IRS1 antibody

Immunohistochemical analysis of IRS1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Rabbit Anti-IRS1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS820213

Immunofluorescence (IF) of anti-IRS1 antibody

Immunofluorescent analysis of IRS1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Rabbit Anti-IRS1 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS820213

Other Notes

Small volumes of anti-IRS1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for IRS1. It may not necessarily be applicable to this product.

NCBI GI #
5031805
NCBI GeneID
3667
NCBI Accession #
NP_005535.1
UniProt Primary Accession #
P35568
UniProt Secondary Accession #
P35569; P35570
UniProt Related Accession #
P35568
GenBank Nucleotide #
NM_005544.2
NCBI Official Full Name
insulin receptor substrate 1
NCBI Official Synonym Full Names
insulin receptor substrate 1
NCBI Official Symbol
IRS1
NCBI Official Synonym Symbols
HIRS-1
UniProt Protein Name
Insulin receptor substrate 1
Protein Family
Isoleucine--tRNA ligase
UniProt Gene Name
IRS1
UniProt Synonym Gene Names
IRS-1
UniProt Entry Name
IRS1_HUMAN
NCBI Protein Information
insulin receptor substrate 1; IRS-1

NCBI Summary for IRS1

This gene encodes a protein which is phosphorylated by insulin receptor tyrosine kinase. Mutations in this gene are associated with type II diabetes and susceptibility to insulin resistance. [provided by RefSeq, Nov 2009]

UniProt Comments for IRS1

IRS1: an adaptor protein that is one of the major substrates of the insulin receptor kinase. Contains multiple tyrosine phosphorylation motifs that serve as docking sites for SH2-domain-containing proteins including phosphatidylinositol 3-kinase p85 subunit and GRB-2. Phosphorylated on ser/thr residues by kinases including JNK and IKK.

Protein type: Adaptor/scaffold; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 2q36

Cellular Component: intracellular membrane-bound organelle; cytoplasm; plasma membrane; caveola; cytosol; nucleus

Molecular Function: insulin-like growth factor receptor binding; signal transducer activity; protein binding; protein kinase C binding; phosphoinositide 3-kinase binding; SH2 domain binding; transmembrane receptor protein tyrosine kinase adaptor protein activity; insulin receptor binding

Biological Process: response to peptide hormone stimulus; phosphoinositide 3-kinase cascade; nerve growth factor receptor signaling pathway; positive regulation of glycogen biosynthetic process; negative regulation of insulin receptor signaling pathway; signal transduction; glucose homeostasis; response to insulin stimulus; negative regulation of insulin secretion; positive regulation of glucose import; mammary gland development; positive regulation of cell proliferation; positive regulation of mesenchymal cell proliferation; protein kinase B signaling cascade; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; positive regulation of phosphoinositide 3-kinase activity; positive regulation of fatty acid beta-oxidation; insulin-like growth factor receptor signaling pathway; cellular response to insulin stimulus; regulation of gene expression; insulin receptor signaling pathway; innate immune response; positive regulation of insulin receptor signaling pathway; lipid catabolic process; positive regulation of cell migration

Disease: Diabetes Mellitus, Noninsulin-dependent
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Research Articles on IRS1

1. study confirms in a large and ethnically homogeneous sample that IRS1 G972R polymorphism is associated with failure to oral antidiabetes drugs among patients with type 2 diabetes

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-IRS1 antibody

 Reference Product  PubMed Publications
 IGF1R antibody  >244 publications with IRS1 and IGF1R
 GRB2 antibody  >138 publications with IRS1 and GRB2
 JAK2 antibody  >62 publications with IRS1 and JAK2
 INSR antibody  >56 publications with IRS1 and INSR
 PTPN11 antibody  >42 publications with IRS1 and PTPN11
 JAK1 antibody  >26 publications with IRS1 and JAK1
 MAPK8 antibody  >23 publications with IRS1 and MAPK8
 PIK3R1 antibody  >12 publications with IRS1 and PIK3R1
 PIK3CA antibody  >6 publications with IRS1 and PIK3CA

Pathways associated with anti-IRS1 antibody

 Products by Pathway  Pathway Diagram
 AGE/RAGE Pathway antibodies  AGE/RAGE Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 Aldosterone-regulated Sodium Reabsorption Pathway antibodies  Aldosterone-regulated Sodium Reabsorption Pathway Diagram
 Aldosterone-regulated Sodium Reabsorption Pathway antibodies  Aldosterone-regulated Sodium Reabsorption Pathway Diagram
 Alpha6-Beta4 Integrin Signaling Pathway antibodies  Alpha6-Beta4 Integrin Signaling Pathway Diagram

Diseases associated with anti-IRS1 antibody

 Disease Name  Pubmed Publications
 Insulin Resistance Antibodies  >1243 publications with IRS1 and Insulin Resistance
 Hyperinsulinism Antibodies  >843 publications with IRS1 and Hyperinsulinism
 Diabetes Mellitus Antibodies  >502 publications with IRS1 and Diabetes Mellitus
 Obesity Antibodies  >420 publications with IRS1 and Obesity
 Diabetes Mellitus, Type 2 Antibodies  >342 publications with IRS1 and Diabetes Mellitus, Type 2
 Disease Models, Animal Antibodies  >204 publications with IRS1 and Disease Models, Animal
 Hyperglycemia Antibodies  >183 publications with IRS1 and Hyperglycemia
 Necrosis Antibodies  >176 publications with IRS1 and Necrosis
 Liver Diseases Antibodies  >135 publications with IRS1 and Liver Diseases
 Inflammation Antibodies  >134 publications with IRS1 and Inflammation

Organs/Tissues associated with anti-IRS1 antibody

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >864 publications with IRS1 and Muscle
 Liver Antibodies  >489 publications with IRS1 and Liver
 Connective Tissue Antibodies  >245 publications with IRS1 and Connective Tissue
 Pancreas Antibodies  >145 publications with IRS1 and Pancreas
 Heart Antibodies  >138 publications with IRS1 and Heart
 Brain Antibodies  >125 publications with IRS1 and Brain
 Ovary Antibodies  >112 publications with IRS1 and Ovary
 Kidney Antibodies  >96 publications with IRS1 and Kidney
 Bone Antibodies  >46 publications with IRS1 and Bone
 Nerve Antibodies  >45 publications with IRS1 and Nerve
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