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ADRBK1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS820280

MBS820280 is an antibody targeting ADRBK1. Also known as Anti-GRK2 Antibody. Reactive in Human, Mouse, Rat, Bovine, Dog, Pig. Host: Rabbit. Rabbit polyclonal antibody to GRK2 More details

$225 / 0.03 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
GRK2 (ADRBK1), Polyclonal Antibody
Full Product Name
Anti-GRK2 Antibody
Product Gene Name
anti-ADRBK1 antibody
Product Synonym Names
BARK; BARK1; Beta-adrenergic receptor kinase 1; Beta-ARK-1; G-protein coupled receptor kinase 2
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat, Bovine, Dog, Pig
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Specificity
Recognizes endogenous levels of GRK2 protein.
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
Antibody/Peptide Pairs
GRK2 peptide (MBS823392) is used for blocking the activity of GRK2 antibody (MBS820280)
Molecular Weight
79,574 Da
OMIM
109635
3D Structure
ModBase 3D Structure for P25098
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human GRK2. The exact sequence is proprietary.
Applications Tested/Suitable for anti-ADRBK1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
Application Notes for anti-ADRBK1 antibody
WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/ICC (1/100 - 1/500)

Western Blot (WB) of anti-ADRBK1 antibody

Western blot analysis of GRK2 expression in HeLa (A), HEK293T (B), NIH3T3 (C), H9C2 (D) whole cell lysates.
Rabbit Anti-ADRBK1 Polyclonal Antibody – Cat# MBS820280

Immunohistochemistry (IHC) of anti-ADRBK1 antibody

Immunohistochemical analysis of GRK2 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Rabbit Anti-ADRBK1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS820280

Immunofluorescence (IF) of anti-ADRBK1 antibody

Immunofluorescent analysis of GRK2 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Rabbit Anti-ADRBK1 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS820280

Other Notes

Small volumes of anti-ADRBK1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ADRBK1. It may not necessarily be applicable to this product.

NCBI GI #
148539876
NCBI GeneID
156
NCBI Accession #
NP_001610.2
UniProt Primary Accession #
P25098
UniProt Secondary Accession #
Q13837; Q6GTT3; B0ZBE1
UniProt Related Accession #
P25098
GenBank Nucleotide #
NM_001619.3
NCBI Official Full Name
beta-adrenergic receptor kinase 1
NCBI Official Synonym Full Names
adrenergic, beta, receptor kinase 1
NCBI Official Symbol
ADRBK1
NCBI Official Synonym Symbols
GRK2; BARK1; BETA-ARK1
UniProt Protein Name
Beta-adrenergic receptor kinase 1
UniProt Synonym Protein Names
G-protein coupled receptor kinase 2
UniProt Gene Name
ADRBK1
UniProt Synonym Gene Names
BARK; BARK1; GRK2; Beta-ARK-1
UniProt Entry Name
ARBK1_HUMAN
NCBI Protein Information
beta-adrenergic receptor kinase 1; beta-ARK-1; G-protein coupled receptor kinase 2

NCBI Summary for ADRBK1

The product of this gene phosphorylates the beta-2-adrenergic receptor and appears to mediate agonist-specific desensitization observed at high agonist concentrations. This protein is an ubiquitous cytosolic enzyme that specifically phosphorylates the activated form of the beta-adrenergic and related G-protein-coupled receptors. Abnormal coupling of beta-adrenergic receptor to G protein is involved in the pathogenesis of the failing heart. [provided by RefSeq, Jul 2008]
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UniProt Comments for ADRBK1

GRK2: a ubiquitous protein kinase of the GRK family. Phosphorylates the beta-2-adrenergic receptor and related G-protein-coupled receptors. Appears to mediate agonist-specific desensitization observed at high agonist concentrations. Expression level is consistently elevated in chronic human heart failure. Mouse models of severe heart failure have been used to demonstrate that inhibition of BARK1 with a peptide inhibitor is sufficient to increase mean survival, reduce dialation and improve cardiac function.

Protein type: Protein kinase, AGC; Kinase, protein; EC 2.7.11.15; Protein kinase, Ser/Thr (non-receptor); AGC group; GRK family; BARK subfamily

Chromosomal Location of Human Ortholog: 11q13.1

Cellular Component: membrane; cytoplasm; plasma membrane; cytosol

Molecular Function: G-protein coupled receptor kinase activity; protein binding; Edg-2 lysophosphatidic acid receptor binding; beta-adrenergic receptor kinase activity; alpha-2A adrenergic receptor binding; ATP binding; protein kinase activity

Biological Process: epidermal growth factor receptor signaling pathway; desensitization of G-protein coupled receptor protein signaling pathway; tachykinin signaling pathway; acetylcholine receptor signaling, muscarinic pathway; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; heart development; peptidyl-threonine phosphorylation; signal transduction; negative regulation of striated muscle contraction; peptidyl-serine phosphorylation; negative regulation of the force of heart contraction by chemical signal; phospholipase C activation; receptor internalization; innate immune response; positive regulation of catecholamine secretion; cardiac muscle contraction
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Research Articles on ADRBK1

1. Low levels of GRK2/GRK5 causes a slow and not complete desensitization/down-regulation of GPR17.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ADRBK1 antibody

 Reference Product  PubMed Publications
 ADRB2 antibody  >57 publications with ADRBK1 and ADRB2
 RHO antibody  >11 publications with ADRBK1 and RHO
 GIT1 antibody  >9 publications with ADRBK1 and GIT1
 CXCR4 antibody  >6 publications with ADRBK1 and CXCR4
 CCR5 antibody  >2 publications with ADRBK1 and CCR5
 BDKRB2 antibody  >1 publications with ADRBK1 and BDKRB2

Pathways associated with anti-ADRBK1 antibody

 Products by Pathway  Pathway Diagram
 CXCR4-mediated Signaling Events Pathway antibodies  CXCR4-mediated Signaling Events Pathway Diagram
 Ca-dependent Events Pathway antibodies  Ca-dependent Events Pathway Diagram
 CaM Pathway antibodies  CaM Pathway Diagram
 Calmodulin Induced Events Pathway antibodies  Calmodulin Induced Events Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 DAG And IP3 Signaling Pathway antibodies  DAG And IP3 Signaling Pathway Diagram
 DAP12 Interactions Pathway antibodies  DAP12 Interactions Pathway Diagram
 DAP12 Signaling Pathway antibodies  DAP12 Signaling Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram

Diseases associated with anti-ADRBK1 antibody

 Disease Name  Pubmed Publications
 Heart Diseases Antibodies  >99 publications with ADRBK1 and Heart Diseases
 Inflammation Antibodies  >54 publications with ADRBK1 and Inflammation
 Hypertension Antibodies  >44 publications with ADRBK1 and Hypertension
 Myocardial Ischemia Antibodies  >35 publications with ADRBK1 and Myocardial Ischemia
 Pain Antibodies  >28 publications with ADRBK1 and Pain
 Myocardial Infarction Antibodies  >27 publications with ADRBK1 and Myocardial Infarction
 Cardiomegaly Antibodies  >16 publications with ADRBK1 and Cardiomegaly
 Cardiomyopathies Antibodies  >14 publications with ADRBK1 and Cardiomyopathies
 Postoperative Complications Antibodies  >13 publications with ADRBK1 and Postoperative Complications
 Necrosis Antibodies  >12 publications with ADRBK1 and Necrosis

Organs/Tissues associated with anti-ADRBK1 antibody

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >123 publications with ADRBK1 and Muscle
 Blood Antibodies  >112 publications with ADRBK1 and Blood
 Brain Antibodies  >98 publications with ADRBK1 and Brain
 Kidney Antibodies  >66 publications with ADRBK1 and Kidney
 Vascular Antibodies  >66 publications with ADRBK1 and Vascular
 Nerve Antibodies  >24 publications with ADRBK1 and Nerve
 Lung Antibodies  >15 publications with ADRBK1 and Lung
 Bone Antibodies  >15 publications with ADRBK1 and Bone
 Liver Antibodies  >14 publications with ADRBK1 and Liver
 Embryonic Tissue Antibodies  >13 publications with ADRBK1 and Embryonic Tissue
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