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NEURL1 (NEURL) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS820324

MBS820324 is an antibody targeting NEURL. Also known as Anti-NEURL1 Antibody. Reactive in Human. Host: Rabbit. Rabbit polyclonal antibody to NEURL1 More details

$225 / 0.03 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
NEURL1, Polyclonal Antibody
Full Product Name
Anti-NEURL1 Antibody
Product Gene Name
anti-NEURL antibody
Product Synonym Names
NEURL; NEURL1A; RNF67; E3 ubiquitin-protein ligase NEURL1; Neuralized-like protein 1A; h-neu; h-neuralized 1; RING finger protein 67
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Specificity
Recognizes endogenous levels of NEURL1 protein.
Preparation and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
Antibody/Peptide Pairs
NEURL1 peptide (MBS822956) is used for blocking the activity of NEURL1 antibody (MBS820324)
OMIM
603804
3D Structure
ModBase 3D Structure for O76050
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human NEURL1. The exact sequence is proprietary.
Applications Tested/Suitable for anti-NEURL antibody
WB, IH, IF/IC
Application Notes for anti-NEURL antibody
WB (1/500 - 1/1000)
IH (1/100 - 1/200)
IF/IC (1/100 - 1/500)

Western Blot (WB) of anti-NEURL antibody

Western blot analysis of NEURL1 expression in Y79 (A), MCF7 (B), HepG2 (C) whole cell lysates.
Rabbit Anti-NEURL Polyclonal Antibody – Cat# MBS820324

Immunohistochemistry (IHC) of anti-NEURL antibody

Immunohistochemical analysis of NEURL1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Rabbit Anti-NEURL Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS820324

Immunofluorescence (IF) of anti-NEURL antibody

Immunofluorescent analysis of NEURL1 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Rabbit Anti-NEURL Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS820324

Other Notes

Small volumes of anti-NEURL antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for NEURL. It may not necessarily be applicable to this product.

NCBI GI #
223278368
NCBI GeneID
9148
NCBI Accession #
NP_004201.3
UniProt Primary Accession #
O76050
UniProt Secondary Accession #
Q5TDR2; Q5TDR3; Q8TAN0; Q9H463
UniProt Related Accession #
O76050
GenBank Nucleotide #
NM_004210.4
NCBI Official Full Name
E3 ubiquitin-protein ligase NEURL1
NCBI Official Synonym Full Names
neuralized E3 ubiquitin protein ligase 1
NCBI Official Symbol
NEURL1
NCBI Official Synonym Symbols
neu; NEUR1; NEURL; RNF67; neu-1; bA416N2.1
UniProt Protein Name
E3 ubiquitin-protein ligase NEURL1
UniProt Synonym Protein Names
Neuralized-like protein 1A; h-neu; h-neuralized 1; RING finger protein 67
Protein Family
E3 ubiquitin-protein ligase
UniProt Gene Name
NEURL1
UniProt Synonym Gene Names
NEURL; NEURL1A; RNF67; h-neu; h-neuralized 1
UniProt Entry Name
NEUL1_HUMAN
NCBI Protein Information
E3 ubiquitin-protein ligase NEURL1; h-neuralized 1; neuralized homolog; RING finger protein 67; neuralized-like protein 1A

UniProt Comments for NEURL

NEURL1: May function as an E3 ubiquitin-protein ligase to activate monoubiquitination of JAG1, thereby regulating the Notch pathway. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Ligase; Cell development/differentiation; Tumor suppressor; EC 6.3.2.-; Ubiquitin conjugating system; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 10q25.1

Cellular Component: postsynaptic membrane; perinuclear region of cytoplasm; postsynaptic density; plasma membrane; dendritic spine; perikaryon; cell junction

Molecular Function: translation factor activity, non-nucleic acid binding; zinc ion binding; ubiquitin-protein ligase activity; ligase activity

Biological Process: regulation of translation; positive regulation of filopodium formation; protein monoubiquitination; lactation; nervous system development; negative regulation of cell proliferation; Notch signaling pathway; sperm motility; positive regulation of epidermal growth factor receptor activity; positive regulation of apoptosis; sperm axoneme assembly; negative regulation of Notch signaling pathway; positive regulation of long-term neuronal synaptic plasticity
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Research Articles on NEURL

1. NEURL1 expression was downregulated in medulloblastoma compared to normal cerebellar tissue with lowest expression levels in hedgehog-activated tumors; conclude NEURL1 is candidate tumor suppressor in MB at least in part through effects on Notch pathway

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-NEURL antibody

 Products by Pathway  Pathway Diagram
 Activated NOTCH1 Transmits Signal To The Nucleus Pathway antibodies  Activated NOTCH1 Transmits Signal To The Nucleus Pathway Diagram
 Constitutive Signaling By NOTCH1 HD Domain Mutants Pathway antibodies  Constitutive Signaling By NOTCH1 HD Domain Mutants Pathway Diagram
 Constitutive Signaling By NOTCH1 HD+PEST Domain Mutants Pathway antibodies  Constitutive Signaling By NOTCH1 HD+PEST Domain Mutants Pathway Diagram
 Constitutive Signaling By NOTCH1 PEST Domain Mutants Pathway antibodies  Constitutive Signaling By NOTCH1 PEST Domain Mutants Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 FBXW7 Mutants And NOTCH1 In Cancer Pathway antibodies  FBXW7 Mutants And NOTCH1 In Cancer Pathway Diagram
 NOTCH2 Activation And Transmission Of Signal To The Nucleus Pathway antibodies  NOTCH2 Activation And Transmission Of Signal To The Nucleus Pathway Diagram
 Notch Signaling Pathway antibodies  Notch Signaling Pathway Diagram
 Signal Transduction Pathway antibodies  Signal Transduction Pathway Diagram
 Signaling By NOTCH Pathway antibodies  Signaling By NOTCH Pathway Diagram

Organs/Tissues associated with anti-NEURL antibody

 Organ/Tissue Name  Pubmed Publications
 Thyroid Antibodies  >1 publications with NEURL and Thyroid
 Brain Antibodies  >1 publications with NEURL and Brain
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