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CES2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS820448

MBS820448 is an antibody targeting CES2. Reactive in Human, Mouse, Rat. Host: Rabbit. Rabbit polyclonal antibody to CES2 More details

$225 / 0.03 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CES2, Polyclonal Antibody
Full Product Name
Anti-CES2 Antibody
Product Gene Name
Product Synonym Names
ICE; Cocaine esterase; Carboxylesterase 2; CE-2; hCE-2; Methylumbelliferyl-acetate deacetylase 2
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Specificity
Recognizes endogenous levels of CES2 protein.
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
Antibody/Peptide Pairs
CES2 peptide (MBS823659) is used for blocking the activity of CES2 antibody (MBS820448)
Molecular Weight
59,959 Da
OMIM
605278
3D Structure
ModBase 3D Structure for O00748
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CES2. The exact sequence is proprietary.
Applications Tested/Suitable for anti-CES2 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-CES2 antibody
WB (1/500 - 1/1000), IH (1/100 - 1/200)

Western Blot (WB) of anti-CES2 antibody

Western blot analysis of CES2 expression in HepG2 (A), SP2/0 (B), H9C2 (C) whole cell lysates.
Rabbit Anti-CES2 Polyclonal Antibody – Cat# MBS820448

Immunohistochemistry (IHC) of anti-CES2 antibody

Immunohistochemical analysis of CES2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Rabbit Anti-CES2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS820448

Other Notes

Small volumes of anti-CES2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CES2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
cocaine esterase isoform 1
NCBI Official Synonym Full Names
carboxylesterase 2
NCBI Official Symbol
CES2
NCBI Official Synonym Symbols
iCE; CE-2; PCE-2; CES2A1
UniProt Protein Name
Cocaine esterase
UniProt Synonym Protein Names
Carboxylesterase 2 (EC:3.1.1.1); CE-2; hCE-2; Methylumbelliferyl-acetate deacetylase 2 (EC:3.1.1.56)
UniProt Gene Name
CES2
UniProt Synonym Gene Names
ICE; CE-2; hCE-2
UniProt Entry Name
EST2_HUMAN
NCBI Protein Information
cocaine esterase; hCE-2; carboxylesterase 2 (intestine, liver); methylumbelliferyl-acetate deacetylase 2; intestinal carboxylesterase; liver carboxylesterase-2

NCBI Summary for CES2

This gene encodes a member of the carboxylesterase large family. The family members are responsible for the hydrolysis or transesterification of various xenobiotics, such as cocaine and heroin, and endogenous substrates with ester, thioester, or amide bonds. They may participate in fatty acyl and cholesterol ester metabolism, and may play a role in the blood-brain barrier system. The protein encoded by this gene is the major intestinal enzyme and functions in intestine drug clearance. Alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Oct 2010]
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UniProt Comments for CES2

CES2: Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Shows high catalytic efficiency for hydrolysis of cocaine, 4-methylumbelliferyl acetate, heroin and 6-monoacetylmorphine. Belongs to the type-B carboxylesterase/lipase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.1.1; Xenobiotic Metabolism - drug metabolism - other enzymes; EC 3.1.1.56; EC 3.1.1.84; Endoplasmic reticulum; Hydrolase

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: extracellular space; endoplasmic reticulum; endoplasmic reticulum lumen

Molecular Function: methylumbelliferyl-acetate deacetylase activity

Biological Process: catabolic process
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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