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COPS3 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS820475

MBS820475 is an antibody targeting COPS3. Reactive in Human, Mouse, Rat, Bovine, Dog, Monkey, Pig. Host: Rabbit. Rabbit polyclonal antibody to COPS3 More details

$225 / 0.03 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
COPS3, Polyclonal Antibody
Full Product Name
Anti-COPS3 Antibody
Product Gene Name
anti-COPS3 antibody
Product Synonym Names
CSN3; COP9 signalosome complex subunit 3; SGN3; Signalosome subunit 3; JAB1-containing signalosome subunit 3
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat, Bovine, Dog, Monkey, Pig
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Specificity
Recognizes endogenous levels of COPS3 protein.
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
Antibody/Peptide Pairs
COPS3 peptide (MBS824272) is used for blocking the activity of COPS3 antibody (MBS820475)
Molecular Weight
45,727 Da
OMIM
604665
3D Structure
ModBase 3D Structure for Q9UNS2
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human COPS3. The exact sequence is proprietary.
Applications Tested/Suitable for anti-COPS3 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
Application Notes for anti-COPS3 antibody
WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/ICC (1/100 - 1/500)

Western Blot (WB) of anti-COPS3 antibody

Western blot analysis of COPS3 expression in HeLa (A), SP2/0 (B), mouse liver (C), rat liver (D), NIH3T3 (E), HEK293T (F) whole cell lysates.
Rabbit Anti-COPS3 Polyclonal Antibody – Cat# MBS820475

Immunohistochemistry (IHC) of anti-COPS3 antibody

Immunohistochemical analysis of COPS3 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Rabbit Anti-COPS3 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS820475

Immunofluorescence (IF) of anti-COPS3 antibody

Immunofluorescent analysis of COPS3 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Rabbit Anti-COPS3 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS820475

Other Notes

Small volumes of anti-COPS3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for COPS3. It may not necessarily be applicable to this product.

NCBI GI #
312284090
NCBI GeneID
8533
NCBI Accession #
NP_001186054.1
UniProt Primary Accession #
Q9UNS2
UniProt Secondary Accession #
O43191; Q7LDR6; B2R683; B4DY81
UniProt Related Accession #
Q9UNS2
GenBank Nucleotide #
NM_001199125.1
NCBI Official Full Name
COP9 signalosome complex subunit 3 isoform 2
NCBI Official Synonym Full Names
COP9 signalosome subunit 3
NCBI Official Symbol
COPS3
NCBI Official Synonym Symbols
CSN3; SGN3
UniProt Protein Name
COP9 signalosome complex subunit 3
UniProt Synonym Protein Names
JAB1-containing signalosome subunit 3
UniProt Gene Name
COPS3
UniProt Synonym Gene Names
CSN3; SGN3; Signalosome subunit 3
UniProt Entry Name
CSN3_HUMAN
NCBI Protein Information
COP9 signalosome complex subunit 3; signalosome subunit 3; COP9 complex subunit 3; JAB1-containing signalosome subunit 3; COP9 constitutive photomorphogenic homolog subunit 3

NCBI Summary for COPS3

The protein encoded by this gene possesses kinase activity that phosphorylates regulators involved in signal transduction. It phosphorylates I kappa-Balpha, p105, and c-Jun. It acts as a docking site for complex-mediated phosphorylation. The gene is located within the Smith-Magenis syndrome region on chromosome 17. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2010]
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UniProt Comments for COPS3

COPS3: Component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. Belongs to the CSN3 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 17p11.2

Cellular Component: nucleoplasm; signalosome; cytoplasm; nucleus

Molecular Function: protein binding

Biological Process: ubiquitin-dependent protein catabolic process; response to light stimulus; cullin deneddylation; in utero embryonic development; signal transduction
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Research Articles on COPS3

1. this study demonstrates Csn3 as an oncogene that regulates the tumorigenesis process in hepatocellular carcinoma (HCC) cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-COPS3 antibody

 Products by Pathway  Pathway Diagram
 TNF-alpha/NF-kB Signaling Pathway antibodies  TNF-alpha/NF-kB Signaling Pathway Diagram

Diseases associated with anti-COPS3 antibody

 Disease Name  Pubmed Publications
 Necrosis Antibodies  >2 publications with COPS3 and Necrosis

Organs/Tissues associated with anti-COPS3 antibody

 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >7 publications with COPS3 and Bone
 Lung Antibodies  >2 publications with COPS3 and Lung
 Bone Marrow Antibodies  >1 publications with COPS3 and Bone Marrow
 Testis Antibodies  >1 publications with COPS3 and Testis
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