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XRCC1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS820509

MBS820509 is an antibody targeting XRCC1. Reactive in Human, Rat, Monkey. Host: Rabbit. Rabbit polyclonal antibody to XRCC1 More details

$225 / 0.03 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
XRCC1, Polyclonal Antibody
Full Product Name
Anti-XRCC1 Antibody
Product Gene Name
Product Synonym Names
DNA repair protein XRCC1; X-ray repair cross-complementing protein 1
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Rat, Monkey
Form/Format
Parvovirus B19 VLP VP1/VP2 protein solution (0.89mg/ml) is supplied in 16mM Sodium phosphate pH 7.4, 120mM NaCl and 20% glycerol
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Specificity
Recognizes endogenous levels of XRCC1 protein.
Preparation and Storage
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
Avoid multiple freeze-thaw cycles.
Shipping: Ships with dry ice.
Antibody/Peptide Pairs
XRCC1 peptide (MBS824266) is used for blocking the activity of XRCC1 antibody (MBS820509)
Molecular Weight
69,477 Da
OMIM
194360
3D Structure
ModBase 3D Structure for P18887
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human XRCC1. The exact sequence is proprietary.
Applications Tested/Suitable for anti-XRCC1 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-XRCC1 antibody
WB (1/500 - 1/1000), IH (1/100 - 1/200)

Western Blot (WB) of anti-XRCC1 antibody

Western blot analysis of XRCC1 expression in MCF7 (A), U2OS (B), A431 (C), SHSY5Y (D), Saos2 (E) whole cell lysates.
Rabbit Anti-XRCC1 Polyclonal Antibody – Cat# MBS820509

Immunohistochemistry (IHC) of anti-XRCC1 antibody

Immunohistochemical analysis of XRCC1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Rabbit Anti-XRCC1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS820509

Other Notes

Small volumes of anti-XRCC1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for XRCC1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
DNA repair protein XRCC1
NCBI Official Synonym Full Names
X-ray repair complementing defective repair in Chinese hamster cells 1
NCBI Official Symbol
XRCC1
NCBI Official Synonym Symbols
RCC
UniProt Protein Name
DNA repair protein XRCC1
UniProt Synonym Protein Names
X-ray repair cross-complementing protein 1
Protein Family
UniProt Gene Name
XRCC1
UniProt Entry Name
XRCC1_HUMAN
NCBI Protein Information
DNA repair protein XRCC1; X-ray repair cross-complementing protein 1

NCBI Summary for XRCC1

The protein encoded by this gene is involved in the efficient repair of DNA single-strand breaks formed by exposure to ionizing radiation and alkylating agents. This protein interacts with DNA ligase III, polymerase beta and poly (ADP-ribose) polymerase to participate in the base excision repair pathway. It may play a role in DNA processing during meiogenesis and recombination in germ cells. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. [provided by RefSeq, Jul 2008]
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UniProt Comments for XRCC1

XRCC1: Corrects defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents. Homodimer. Interacts with polynucleotide kinase (PNK), DNA polymerase-beta (POLB) and DNA ligase III (LIG3). Interacts with APTX and APLF. Interacts with APEX1; the interaction is induced by SIRT1 and increases with the acetylated form of APEX1.

Protein type: DNA repair, damage

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: nucleoplasm; nucleus

Molecular Function: protein binding; enzyme binding; damaged DNA binding

Biological Process: single strand break repair; base-excision repair; DNA repair
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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