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DGK Alpha (DGKA) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS820559

MBS820559 is an antibody targeting DGKA. Also known as Anti-DGK alpha Antibody. Reactive in Human, Monkey. Host: Rabbit. Rabbit polyclonal antibody to DGK alpha More details

$225 / 0.03 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
DGK alpha (DGKA), Polyclonal Antibody
Full Product Name
Anti-DGK alpha Antibody
Product Gene Name
anti-DGKA antibody
Product Synonym Names
DAGK; DAGK1; Diacylglycerol kinase alpha; DAG kinase alpha; 80 kDa diacylglycerol kinase; Diglyceride kinase alpha; DGK-alpha
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Monkey
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Specificity
Recognizes endogenous levels of DGK alpha protein.
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
Antibody/Peptide Pairs
DGK alpha peptide (MBS822658) is used for blocking the activity of DGK alpha antibody (MBS820559)
Molecular Weight
28,094 Da
OMIM
125855
3D Structure
ModBase 3D Structure for P23743
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human DGK alpha. The exact sequence is proprietary.
Applications Tested/Suitable for anti-DGKA antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
Application Notes for anti-DGKA antibody
WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/ICC (1/100 - 1/500)

Western Blot (WB) of anti-DGKA antibody

Western blot analysis of DGK alpha expression in MDCK (A), COS7 (B) whole cell lysates.
Rabbit Anti-DGKA Polyclonal Antibody – Cat# MBS820559

Immunohistochemistry (IHC) of anti-DGKA antibody

Immunohistochemical analysis of DGK alpha staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Rabbit Anti-DGKA Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS820559

Immunofluorescence (IF) of anti-DGKA antibody

Immunofluorescent analysis of DGK alpha staining in MDCK cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Rabbit Anti-DGKA Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS820559

Other Notes

Small volumes of anti-DGKA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for DGKA. It may not necessarily be applicable to this product.

NCBI GI #
31542504
NCBI GeneID
1606
NCBI Accession #
NP_001336.2
UniProt Primary Accession #
P23743
UniProt Secondary Accession #
O75481; O75482; O75483; O95217; Q3ZE25; Q8IZ56; Q8N5Q2
UniProt Related Accession #
P23743
GenBank Nucleotide #
NM_001345.4
NCBI Official Full Name
diacylglycerol kinase alpha
NCBI Official Synonym Full Names
diacylglycerol kinase, alpha 80kDa
NCBI Official Symbol
DGKA
NCBI Official Synonym Symbols
DAGK; DAGK1; DGK-alpha
UniProt Protein Name
Diacylglycerol kinase alpha
UniProt Synonym Protein Names
80 kDa diacylglycerol kinase; Diglyceride kinase alpha; DGK-alpha
Protein Family
Diacylglycerol kinase
UniProt Gene Name
DGKA
UniProt Synonym Gene Names
DAGK; DAGK1; DAG kinase alpha; DGK-alpha
UniProt Entry Name
DGKA_HUMAN
NCBI Protein Information
diacylglycerol kinase alpha; DAG kinase alpha; diglyceride kinase alpha; 80 kDa diacylglycerol kinase

NCBI Summary for DGKA

The protein encoded by this gene belongs to the eukaryotic diacylglycerol kinase family. It acts as a modulator that competes with protein kinase C for the second messenger diacylglycerol in intracellular signaling pathways. It also plays an important role in the resynthesis of phosphatidylinositols and phosphorylating diacylglycerol to phosphatidic acid. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified. [provided by RefSeq, Jul 2008]
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UniProt Comments for DGKA

DGKA: Upon cell stimulation converts the second messenger diacylglycerol into phosphatidate, initiating the resynthesis of phosphatidylinositols and attenuating protein kinase C activity. Monomer. Lymphocytes and oligodendroglial cells. Stimulated by calcium and phosphatidylserine. Phosphorylated by protein kinase C. Belongs to the eukaryotic diacylglycerol kinase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.7.1.107; Kinase, lipid; Lipid Metabolism - glycerolipid; Lipid Metabolism - glycerophospholipid; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 12q13.3

Cellular Component: membrane; plasma membrane; cytosol

Molecular Function: phospholipid binding; calcium ion binding; diacylglycerol kinase activity; ATP binding; NAD+ kinase activity

Biological Process: platelet activation; protein kinase C activation; blood coagulation; phosphorylation
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Research Articles on DGKA

1. High diacylglycerol kinase alpha expression is associated with glioblastoma.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-DGKA antibody

 Reference Product  PubMed Publications
 AGPAT4 antibody  >1 publications with DGKA and AGPAT4

Pathways associated with anti-DGKA antibody

 Products by Pathway  Pathway Diagram
 Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway antibodies  Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway Diagram
 Effects Of PIP2 Hydrolysis Pathway antibodies  Effects Of PIP2 Hydrolysis Pathway Diagram
 G Alpha (q) Signalling Events Pathway antibodies  G Alpha (q) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 Gastrin-CREB Signalling Pathway Via PKC And MAPK antibodies  Gastrin-CREB Signalling Pathway Via PKC And MAPK Diagram
 Glycerolipid Metabolism Pathway antibodies  Glycerolipid Metabolism Pathway Diagram
 Glycerolipid Metabolism Pathway antibodies  Glycerolipid Metabolism Pathway Diagram
 Glycerophospholipid Metabolism Pathway antibodies  Glycerophospholipid Metabolism Pathway Diagram
 Glycerophospholipid Metabolism Pathway antibodies  Glycerophospholipid Metabolism Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram

Organs/Tissues associated with anti-DGKA antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >4 publications with DGKA and Blood
 Bone Antibodies  >1 publications with DGKA and Bone
 Embryonic Tissue Antibodies  >1 publications with DGKA and Embryonic Tissue
 Liver Antibodies  >1 publications with DGKA and Liver
 Stomach Antibodies  >1 publications with DGKA and Stomach
 Muscle Antibodies  >1 publications with DGKA and Muscle
 Lung Antibodies  >1 publications with DGKA and Lung
 Esophagus Antibodies  >1 publications with DGKA and Esophagus
 Bone Marrow Antibodies  >1 publications with DGKA and Bone Marrow
 Uterus Antibodies  >1 publications with DGKA and Uterus
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