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NEDD8 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS820582

MBS820582 is an antibody targeting NEDD8. Reactive in Human, Mouse, Rat, Bovine, Dog, Rabbit. Host: Rabbit. Rabbit polyclonal antibody to NEDD8 More details

$225 / 0.03 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
NEDD8, Polyclonal Antibody
Full Product Name
Anti-NEDD8 Antibody
Product Gene Name
anti-NEDD8 antibody
Product Synonym Names
NEDD8; Neddylin; Neural precursor cell expressed developmentally down-regulated protein 8; NEDD-8; Ubiquitin-like protein Nedd8
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat, Bovine, Dog, Rabbit
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Specificity
Recognizes endogenous levels of NEDD8 protein.
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
Antibody/Peptide Pairs
NEDD8 peptide (MBS822925) is used for blocking the activity of NEDD8 antibody (MBS820582)
Molecular Weight
9,072 Da
OMIM
603171
3D Structure
ModBase 3D Structure for Q15843
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human NEDD8. The exact sequence is proprietary.
Applications Tested/Suitable for anti-NEDD8 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
Application Notes for anti-NEDD8 antibody
WB (1/500 - 1/1000), IH (1/100 - 1/200), IP (1/10 - 1/100)

Western Blot (WB) of anti-NEDD8 antibody

Western blot analysis of NEDD8 expression in BJAB (A), K562 (B), HeLa (C) whole cell lysates.
Rabbit Anti-NEDD8 Polyclonal Antibody – Cat# MBS820582

Immunohistochemistry (IHC) of anti-NEDD8 antibody

Immunohistochemical analysis of NEDD8 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Rabbit Anti-NEDD8 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS820582

Other Notes

Small volumes of anti-NEDD8 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for NEDD8. It may not necessarily be applicable to this product.

NCBI GI #
5453760
NCBI GeneID
4738
NCBI Accession #
NP_006147.1
UniProt Primary Accession #
Q15843
UniProt Secondary Accession #
Q3SXN8; Q6LES6
UniProt Related Accession #
Q15843
GenBank Nucleotide #
NM_006156.2
NCBI Official Full Name
NEDD8
NCBI Official Synonym Full Names
neural precursor cell expressed, developmentally down-regulated 8
NCBI Official Symbol
NEDD8
NCBI Official Synonym Symbols
NEDD-8
UniProt Protein Name
NEDD8
UniProt Synonym Protein Names
Neddylin; Neural precursor cell expressed developmentally down-regulated protein 8; NEDD-8; Ubiquitin-like protein Nedd8
Protein Family
NEDD8
UniProt Gene Name
NEDD8
UniProt Synonym Gene Names
NEDD-8
UniProt Entry Name
NEDD8_HUMAN
NCBI Protein Information
NEDD8; neddylin; ubiquitin-like protein Nedd8

UniProt Comments for NEDD8

NEDD8: a member of the ubiquitin family of proteins. Approximately 60% identical to ubiquitin protein. Activated by an E1-like complex, consisting of App-b1 and Uba3 and then linked to the E2-like enzyme, Ubc12. The major target protein modified by nedd8 is cullin-4a. Down-regulated during the development of brain.

Protein type: Ubiquitin-like modifier

Chromosomal Location of Human Ortholog: 14q12

Cellular Component: nucleus; cytosol

Molecular Function: protein binding; ubiquitin protein ligase binding

Biological Process: ubiquitin-dependent protein catabolic process; regulation of transcription from RNA polymerase II promoter; protein localization; anatomical structure morphogenesis; protein neddylation; transforming growth factor beta receptor signaling pathway; protein modification process; proteolysis; response to organic cyclic substance
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Research Articles on NEDD8

1. Study reports the structure of a trapped RING E3-E2 approximately UBL-target intermediate representing RBX1-UBC12 approximately NEDD8-CUL1-DCN1, which reveals the mechanism of NEDD8 ligation and how a particular UBL and acceptor lysine are matched by a multifunctional RING E3.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-NEDD8 antibody

 Reference Product  PubMed Publications
 CUL1 antibody  >62 publications with NEDD8 and CUL1
 UBA3 antibody  >41 publications with NEDD8 and UBA3
 NUB1 antibody  >26 publications with NEDD8 and NUB1
 CUL3 antibody  >22 publications with NEDD8 and CUL3
 CUL2 antibody  >21 publications with NEDD8 and CUL2
 SENP8 antibody  >18 publications with NEDD8 and SENP8
 CUL5 antibody  >12 publications with NEDD8 and CUL5
 TP53 antibody  >7 publications with NEDD8 and TP53
 UBE2M antibody  >4 publications with NEDD8 and UBE2M
 UBC antibody  >1 publications with NEDD8 and UBC

Pathways associated with anti-NEDD8 antibody

 Products by Pathway  Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Loss Of Function Of SMAD2/3 In Cancer Pathway antibodies  Loss Of Function Of SMAD2/3 In Cancer Pathway Diagram
 Loss Of Function Of SMAD4 In Cancer Pathway antibodies  Loss Of Function Of SMAD4 In Cancer Pathway Diagram
 Loss Of Function Of TGFBR1 In Cancer Pathway antibodies  Loss Of Function Of TGFBR1 In Cancer Pathway Diagram
 Loss Of Function Of TGFBR2 In Cancer Pathway antibodies  Loss Of Function Of TGFBR2 In Cancer Pathway Diagram
 SMAD2/3 MH2 Domain Mutants In Cancer Pathway antibodies  SMAD2/3 MH2 Domain Mutants In Cancer Pathway Diagram
 SMAD2/3 Phosphorylation Motif Mutants In Cancer Pathway antibodies  SMAD2/3 Phosphorylation Motif Mutants In Cancer Pathway Diagram
 SMAD4 MH2 Domain Mutants In Cancer Pathway antibodies  SMAD4 MH2 Domain Mutants In Cancer Pathway Diagram
 Signal Transduction Pathway antibodies  Signal Transduction Pathway Diagram
 Signaling By TGF-beta Receptor Complex Pathway antibodies  Signaling By TGF-beta Receptor Complex Pathway Diagram

Diseases associated with anti-NEDD8 antibody

 Disease Name  Pubmed Publications
 Adenocarcinoma Antibodies  >11 publications with NEDD8 and Adenocarcinoma
 Necrosis Antibodies  >10 publications with NEDD8 and Necrosis
 Inflammation Antibodies  >9 publications with NEDD8 and Inflammation
 Kidney Diseases Antibodies  >7 publications with NEDD8 and Kidney Diseases
 Liver Diseases Antibodies  >5 publications with NEDD8 and Liver Diseases
 Liver Neoplasms Antibodies  >4 publications with NEDD8 and Liver Neoplasms
 Cell Transformation, Neoplastic Antibodies  >4 publications with NEDD8 and Cell Transformation, Neoplastic
 Breast Neoplasms Antibodies  >4 publications with NEDD8 and Breast Neoplasms
 Ovarian Neoplasms Antibodies  >4 publications with NEDD8 and Ovarian Neoplasms
 Prostatic Diseases Antibodies  >3 publications with NEDD8 and Prostatic Diseases

Organs/Tissues associated with anti-NEDD8 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >24 publications with NEDD8 and Brain
 Blood Antibodies  >23 publications with NEDD8 and Blood
 Kidney Antibodies  >15 publications with NEDD8 and Kidney
 Nerve Antibodies  >12 publications with NEDD8 and Nerve
 Vascular Antibodies  >11 publications with NEDD8 and Vascular
 Muscle Antibodies  >9 publications with NEDD8 and Muscle
 Lung Antibodies  >9 publications with NEDD8 and Lung
 Heart Antibodies  >8 publications with NEDD8 and Heart
 Embryonic Tissue Antibodies  >8 publications with NEDD8 and Embryonic Tissue
 Liver Antibodies  >8 publications with NEDD8 and Liver
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