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SLC25A31 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS820588

MBS820588 is an antibody targeting SLC25A31. Also known as Anti-ANT4 Antibody. Reactive in Human, Monkey. Host: Rabbit. Rabbit polyclonal antibody to ANT4 More details

$225 / 0.03 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
ANT4 (SLC25A31), Polyclonal Antibody
Full Product Name
Anti-ANT4 Antibody
Product Gene Name
Product Synonym Names
AAC4; ANT4; SFEC; ADP/ATP translocase 4; ADP,ATP carrier protein 4; Adenine nucleotide translocator 4; ANT 4; Solute carrier family 25 member 31; Sperm flagellar energy carrier protein
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Monkey
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Specificity
Recognizes endogenous levels of ANT4 protein.
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
Antibody/Peptide Pairs
ANT4 peptide (MBS822786) is used for blocking the activity of ANT4 antibody (MBS820588)
Molecular Weight
35,022 Da
OMIM
610796
3D Structure
ModBase 3D Structure for Q9H0C2
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human ANT4. The exact sequence is proprietary.
Applications Tested/Suitable for anti-SLC25A31 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemsitry (ICC), Immunoprecipitation (IP)
Application Notes for anti-SLC25A31 antibody
WB (1/500 - 1/1000), IF/ICC (1/100 - 1/500), IP (1/10 - 1/100)

Western Blot (WB) of anti-SLC25A31 antibody

Western blot analysis of ANT4 expression in K562 (A), U251MG (B) whole cell lysates.
Rabbit Anti-SLC25A31 Polyclonal Antibody – Cat# MBS820588

Immunohistochemistry (IHC) of anti-SLC25A31 antibody

Rabbit Anti-SLC25A31 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS820588

Immunofluorescence (IF) of anti-SLC25A31 antibody

Immunofluorescent analysis of ANT4 staining in K562 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Rabbit Anti-SLC25A31 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS820588

Other Notes

Small volumes of anti-SLC25A31 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for SLC25A31. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
ADP/ATP translocase 4
NCBI Official Synonym Full Names
solute carrier family 25 (mitochondrial carrier; adenine nucleotide translocator), member 31
NCBI Official Symbol
SLC25A31
NCBI Official Synonym Symbols
AAC4; ANT4; SFEC35kDa
UniProt Protein Name
ADP/ATP translocase 4
UniProt Synonym Protein Names
ADP,ATP carrier protein 4; Adenine nucleotide translocator 4; ANT 4; Solute carrier family 25 member 31; Sperm flagellar energy carrier protein
Protein Family
UniProt Gene Name
SLC25A31
UniProt Synonym Gene Names
AAC4; ANT4; SFEC; ANT 4
UniProt Entry Name
ADT4_HUMAN
NCBI Protein Information
ADP/ATP translocase 4; ANT 4; ADP,ATP carrier protein 4; adenine nucleotide translocase 4; adenine nucleotide translocator 4; solute carrier family 25 member 31; sperm flagellar energy carrier protein

NCBI Summary for SLC25A31

Mitochondrial ADP/ATP carriers, such as SLC25A31, are nuclear-coded mitochondrial proteins that catalyze the exchange of ATP generated in mitochondria by ATP synthase (see MIM 108729) against ADP produced in cytosol by most energy-consuming reactions (Dolce et al., 2005 [PubMed 15670820]).[supplied by OMIM, Mar 2008]
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UniProt Comments for SLC25A31

SLC25A31: Catalyzes the exchange of cytoplasmic ADP with mitochondrial ATP across the mitochondrial inner membrane. May serve to mediate energy generating and energy consuming processes in the distal flagellum, possibly as a nucleotide shuttle between flagellar glycolysis, protein phosphorylation and mechanisms of motility. Belongs to the mitochondrial carrier family.

Protein type: Transporter; Membrane protein, multi-pass; Membrane protein, integral; Transporter, SLC family; Mitochondrial

Chromosomal Location of Human Ortholog: 4q28.1

Cellular Component: mitochondrion; mitochondrial inner membrane; integral to membrane; nucleus

Molecular Function: transporter activity

Biological Process: transmembrane transport
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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