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RAPGEF5 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS820603

MBS820603 is an antibody targeting RAPGEF5. Also known as Anti-GFR Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit. Rabbit polyclonal antibody to GFR More details

$225 / 0.03 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
GFR (RAPGEF5), Polyclonal Antibody
Full Product Name
Anti-GFR Antibody
Product Gene Name
Product Synonym Names
GFR; KIAA0277; MRGEF; Rap guanine nucleotide exchange factor 5; Guanine nucleotide exchange factor for Rap1; M-Ras-regulated Rap GEF; MR-GEF; Related to Epac; Repac
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Specificity
Recognizes endogenous levels of GFR protein.
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
Antibody/Peptide Pairs
GFR peptide (MBS824142) is used for blocking the activity of GFR antibody (MBS820603)
Molecular Weight
52,083 Da
OMIM
609527
3D Structure
ModBase 3D Structure for Q92565
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human GFR. The exact sequence is proprietary.
Applications Tested/Suitable for anti-RAPGEF5 antibody
Western Blot (WB)
Application Notes for anti-RAPGEF5 antibody
WB (1/500 - 1/1000)

Western Blot (WB) of anti-RAPGEF5 antibody

Western blot analysis of GFR expression in K562 (A), Raw264.7 (B), H9C2 (C) whole cell lysates.
Rabbit Anti-RAPGEF5 Polyclonal Antibody – Cat# MBS820603

Other Notes

Small volumes of anti-RAPGEF5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for RAPGEF5. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
rap guanine nucleotide exchange factor 5
NCBI Official Synonym Full Names
Rap guanine nucleotide exchange factor (GEF) 5
NCBI Official Symbol
RAPGEF5
NCBI Official Synonym Symbols
GFR; REPAC; MR-GEF
UniProt Protein Name
Rap guanine nucleotide exchange factor 5
UniProt Synonym Protein Names
Guanine nucleotide exchange factor for Rap1; M-Ras-regulated Rap GEF; MR-GEF; Related to Epac; Repac
UniProt Gene Name
RAPGEF5
UniProt Synonym Gene Names
GFR; KIAA0277; MRGEF; MR-GEF; Repac
UniProt Entry Name
RPGF5_HUMAN
NCBI Protein Information
rap guanine nucleotide exchange factor 5; related to Epac; M-Ras-regulated GEF; M-Ras-regulated Rap GEF; guanine nucleotide exchange factor for Rap1

NCBI Summary for RAPGEF5

Members of the RAS (see HRAS; MIM 190020) subfamily of GTPases function in signal transduction as GTP/GDP-regulated switches that cycle between inactive GDP- and active GTP-bound states. Guanine nucleotide exchange factors (GEFs), such as RAPGEF5, serve as RAS activators by promoting acquisition of GTP to maintain the active GTP-bound state and are the key link between cell surface receptors and RAS activation (Rebhun et al., 2000 [PubMed 10934204]).[supplied by OMIM, Mar 2008]
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UniProt Comments for RAPGEF5

RAPGEF5: Guanine nucleotide exchange factor (GEF) for RAP1A, RAP2A and MRAS/M-Ras-GTP. Its association with MRAS inhibits Rap1 activation. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: GAPs, Ras; GAPs

Chromosomal Location of Human Ortholog: 7p15.3

Cellular Component: nucleus

Molecular Function: Rap guanyl-nucleotide exchange factor activity; GTP-dependent protein binding; Ras guanyl-nucleotide exchange factor activity

Biological Process: nervous system development; small GTPase mediated signal transduction
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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