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Histamine H2 Receptor (HRH2) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS820672

MBS820672 is an antibody targeting HRH2. Also known as Anti-Histamine H2 Receptor Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit. Rabbit polyclonal antibody to Histamine H2 Receptor More details

$225 / 0.03 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Histamine H2 Receptor (HRH2), Polyclonal Antibody
Full Product Name
Anti-Histamine H2 Receptor Antibody
Product Gene Name
anti-HRH2 antibody
Product Synonym Names
Histamine H2 receptor; H2R; HH2R; Gastric receptor I
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Specificity
Recognizes endogenous levels of Histamine H2 Receptor protein.
Preparation and Storage
Upon receipt aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
Antibody/Peptide Pairs
Histamine H2 Receptor peptide (MBS824383) is used for blocking the activity of Histamine H2 Receptor antibody (MBS820672)
3D Structure
ModBase 3D Structure for P25021
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Histamine H2 Receptor. The exact sequence is proprietary.
Applications Tested/Suitable for anti-HRH2 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)
Application Notes for anti-HRH2 antibody
WB: (1/500 - 1/1000)
IF/ICC: (1/100 - 1/500)

Western Blot (WB) of anti-HRH2 antibody

Western blot analysis of Histamine H2 Receptor expression in mouse kidney (A), rat liver (B) whole cell lysates.
(Predicted band size: 40 kD; Observed band size: 40 kD)
Rabbit Anti-HRH2 Polyclonal Antibody – Cat# MBS820672

Immunofluorescence (IF) of anti-HRH2 antibody

Immunofluorescent analysis of Histamine H2 Receptor staining in COLO205 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Rabbit Anti-HRH2 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS820672

Other Notes

Small volumes of anti-HRH2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for HRH2. It may not necessarily be applicable to this product.

NCBI GI #
197085610
NCBI GeneID
3274
NCBI Accession #
NP_001124527.1
UniProt Primary Accession #
P25021
UniProt Secondary Accession #
Q9D282
UniProt Related Accession #
P25021
GenBank Nucleotide #
NM_001131055.1
NCBI Official Full Name
histamine H2 receptor isoform 1
NCBI Official Synonym Full Names
histamine receptor H2
NCBI Official Symbol
HRH2
NCBI Official Synonym Symbols
H2R
UniProt Protein Name
Histamine H2 receptor
UniProt Synonym Protein Names
Gastric receptor I
Protein Family
Histamine H2 receptor
UniProt Gene Name
Hrh2
UniProt Synonym Gene Names
H2R; HH2R
UniProt Entry Name
HRH2_MOUSE
NCBI Protein Information
histamine H2 receptor; HH2R; gastric receptor 1; gastric receptor I

NCBI Summary for HRH2

Histamine is a ubiquitous messenger molecule released from mast cells, enterochromaffin-like cells, and neurons. Its various actions are mediated by histamine receptors H1, H2, H3 and H4. Histamine receptor H2 belongs to the family 1 of G protein-coupled receptors. It is an integral membrane protein and stimulates gastric acid secretion. It also regulates gastrointestinal motility and intestinal secretion and is thought to be involved in regulating cell growth and differentiation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]
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UniProt Comments for HRH2

H2R: The H2 subclass of histamine receptors mediates gastric acid secretion. Also appears to regulate gastrointestinal motility and intestinal secretion. Possible role in regulating cell growth and differentiation. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase and, through a separate G protein-dependent mechanism, the phosphoinositide/protein kinase (PKC) signaling pathway. Belongs to the G-protein coupled receptor 1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; GPCR, family 1; Receptor, GPCR; Membrane protein, integral

Chromosomal Location of Human Ortholog: 5q35.2

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: histamine receptor activity

Biological Process: gland development; G-protein signaling, coupled to cyclic nucleotide second messenger; gastrin-induced gastric acid secretion; gut development; histamine-induced gastric acid secretion; positive regulation of vasoconstriction; visual learning; regulation of synaptic plasticity; immune response; memory
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Research Articles on HRH2

1. But carriers of one or three copies of HRH1 (5% of individuals), HRH2 (1.1%) and HRH4 genes (0.9%) were also identified.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-HRH2 antibody

 Reference Product  PubMed Publications
 JUN antibody  >4 publications with HRH2 and JUN
 HDC antibody  >2 publications with HRH2 and HDC
 ATP4A antibody  >2 publications with HRH2 and ATP4A

Pathways associated with anti-HRH2 antibody

 Products by Pathway  Pathway Diagram
 Amine Ligand-binding Receptors Pathway antibodies  Amine Ligand-binding Receptors Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 G Alpha (s) Signalling Events Pathway antibodies  G Alpha (s) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 GPCRs, Class A Rhodopsin-like Pathway antibodies  GPCRs, Class A Rhodopsin-like Pathway Diagram
 Gastric Acid Secretion Pathway antibodies  Gastric Acid Secretion Pathway Diagram
 Gastric Acid Secretion Pathway antibodies  Gastric Acid Secretion Pathway Diagram

Diseases associated with anti-HRH2 antibody

 Disease Name  Pubmed Publications
 Drug Hypersensitivity Antibodies  >3 publications with HRH2 and Drug Hypersensitivity
 Inflammation Antibodies  >2 publications with HRH2 and Inflammation
 Pain Antibodies  >2 publications with HRH2 and Pain
 Seizures Antibodies  >2 publications with HRH2 and Seizures
 Neoplasms, Experimental Antibodies  >2 publications with HRH2 and Neoplasms, Experimental
 Ulcer Antibodies  >1 publications with HRH2 and Ulcer
 Peptic Ulcer Antibodies  >1 publications with HRH2 and Peptic Ulcer

Organs/Tissues associated with anti-HRH2 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >3 publications with HRH2 and Brain
 Skin Antibodies  >2 publications with HRH2 and Skin
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