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Cstf-64 (CSTF2) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS820769

MBS820769 is an antibody targeting CSTF2. Also known as Anti-CstF-64 Antibody. Reactive in Human, Mouse, Rat, Bovine, Zebrafish. Host: Rabbit. Rabbit polyclonal antibody to CstF-64 More details

$225 / 0.03 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CstF-64 (CSTF2), Polyclonal Antibody
Full Product Name
Anti-CstF-64 Antibody
Product Gene Name
anti-CSTF2 antibody
Product Synonym Names
Cleavage stimulation factor subunit 2; CF-1 64 kDa subunit; Cleavage stimulation factor 64 kDa subunit; CSTF 64 kDa subunit; CstF-64
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat, Bovine, Zebrafish
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Specificity
Recognizes endogenous levels of CstF-64 protein.
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
Antibody/Peptide Pairs
CstF-64 peptide (MBS823487) is used for blocking the activity of CstF-64 antibody (MBS820769)
Molecular Weight
59,251 Da
OMIM
300907
3D Structure
ModBase 3D Structure for P33240
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human CstF-64. The exact sequence is proprietary.
Applications Tested/Suitable for anti-CSTF2 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP)
Application Notes for anti-CSTF2 antibody
WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/ICC (1/100 - 1/500), IP (1/10 - 1/100)

Western Blot (WB) of anti-CSTF2 antibody

Western blot analysis of Cytochrome P450 1A1/2 expression in MCF7 (A), HeLa (B) whole cell lysates.
Rabbit Anti-CSTF2 Polyclonal Antibody – Cat# MBS820769

Immunohistochemistry (IHC) of anti-CSTF2 antibody

Immunohistochemical analysis of Cytochrome P450 1A1/2 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Rabbit Anti-CSTF2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS820769

Immunofluorescence (IF) of anti-CSTF2 antibody

Immunofluorescent analysis of Cytochrome P450 1A1/2 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Rabbit Anti-CSTF2 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS820769

Other Notes

Small volumes of anti-CSTF2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CSTF2. It may not necessarily be applicable to this product.

NCBI GI #
4557493
NCBI GeneID
1478
NCBI Accession #
NP_001316.1
UniProt Primary Accession #
P33240
UniProt Secondary Accession #
Q5H951; Q6LA74; Q8N502
UniProt Related Accession #
P33240
GenBank Nucleotide #
NM_001325.2
NCBI Official Full Name
cleavage stimulation factor subunit 2
NCBI Official Synonym Full Names
cleavage stimulation factor, 3' pre-RNA, subunit 2, 64kDa
NCBI Official Symbol
CSTF2
NCBI Official Synonym Symbols
CstF-64
UniProt Protein Name
Cleavage stimulation factor subunit 2
UniProt Synonym Protein Names
CF-1 64 kDa subunit; Cleavage stimulation factor 64 kDa subunit; CSTF 64 kDa subunit; CstF-64
Protein Family
Cleavage stimulation factor
UniProt Gene Name
CSTF2
UniProt Synonym Gene Names
CSTF 64 kDa subunit; CstF-64
UniProt Entry Name
CSTF2_HUMAN
NCBI Protein Information
cleavage stimulation factor subunit 2; CF-1 64 kDa subunit; CSTF 64 kDa subunit; betaCstF-64 variant 2; cleavage stimulation factor 64 kDa subunit; cleavage stimulation factor, 3' pre-RNA, subunit 2, 64kD

NCBI Summary for CSTF2

This gene encodes a nuclear protein with an RRM (RNA recognition motif) domain. The protein is a member of the cleavage stimulation factor (CSTF) complex that is involved in the 3' end cleavage and polyadenylation of pre-mRNAs. Specifically, this protein binds GU-rich elements within the 3'-untranslated region of mRNAs. [provided by RefSeq, Jul 2008]
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UniProt Comments for CSTF2

CSTF2: One of the multiple factors required for polyadenylation and 3'-end cleavage of mammalian pre-mRNAs. This subunit is directly involved in the binding to pre-mRNAs. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding; RNA splicing

Chromosomal Location of Human Ortholog: Xq22.1

Cellular Component: nucleoplasm; mRNA cleavage and polyadenylation specificity factor complex; nucleus

Molecular Function: protein binding; RNA binding; nucleotide binding

Biological Process: nuclear mRNA splicing, via spliceosome; transcription from RNA polymerase II promoter; RNA splicing; gene expression; mRNA polyadenylation; mRNA 3'-end processing; termination of RNA polymerase II transcription; mRNA cleavage
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Research Articles on CSTF2

1. CstF64 and CstF64tau modulate one another's expression and play overlapping as well as distinct roles in regulating global alternative polyadenylation profiles.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-CSTF2 antibody

 Products by Pathway  Pathway Diagram
 Cleavage Of Growing Transcript In The Termination Region Pathway antibodies  Cleavage Of Growing Transcript In The Termination Region Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Processing Of Capped Intron-Containing Pre-mRNA Pathway antibodies  Processing Of Capped Intron-Containing Pre-mRNA Pathway Diagram
 Processing Of Capped Intronless Pre-mRNA Pathway antibodies  Processing Of Capped Intronless Pre-mRNA Pathway Diagram
 Processing Of Intronless Pre-mRNAs Pathway antibodies  Processing Of Intronless Pre-mRNAs Pathway Diagram
 RNA Polymerase II Transcription Pathway antibodies  RNA Polymerase II Transcription Pathway Diagram
 RNA Polymerase II Transcription Termination Pathway antibodies  RNA Polymerase II Transcription Termination Pathway Diagram
 MRNA 3'-end Processing Pathway antibodies  MRNA 3'-end Processing Pathway Diagram
 MRNA Splicing Pathway antibodies  MRNA Splicing Pathway Diagram
 MRNA Splicing - Major Pathway antibodies  MRNA Splicing - Major Pathway Diagram

Organs/Tissues associated with anti-CSTF2 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >3 publications with CSTF2 and Brain
 Testis Antibodies  >2 publications with CSTF2 and Testis
 Liver Antibodies  >1 publications with CSTF2 and Liver
 Lung Antibodies  >1 publications with CSTF2 and Lung
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