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CHAT Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS820916

MBS820916 is an antibody targeting CHAT. Reactive in Human, Mouse, Rat, Chicken, Pig, Zebrafish. Host: Rabbit. Rabbit polyclonal antibody to CHAT More details

$225 / 0.03 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CHAT, Polyclonal Antibody
Full Product Name
Anti-CHAT Antibody
Product Gene Name
anti-CHAT antibody
Product Synonym Names
Choline O-acetyltransferase; CHOACTase; ChAT; Choline acetylase
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat, Chicken, Pig, Zebrafish
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Specificity
Recognizes endogenous levels of CHAT protein.
Preparation and Storage
Upon receipt aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.
Antibody/Peptide Pairs
CHAT peptide (MBS824411) is used for blocking the activity of CHAT antibody (MBS820916)
OMIM
gene 254210
3D Structure
ModBase 3D Structure for P28329
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CHAT. The exact sequence is proprietary.
Applications Tested/Suitable for anti-CHAT antibody
Western Blot (WB), Immunofluroescence / Immunocytochemistry (IF/ICC)
Application Notes for anti-CHAT antibody
WB (1/500 - 1/1000)
IF/ICC (1/50 - 1/200)

Western Blot (WB) of anti-CHAT antibody

Western blot analysis of CHAT expression in zebrafish (A) whole cell lysates. (Predicted band size: 82 kD; Observed band size: 82 kD)
Rabbit Anti-CHAT Polyclonal Antibody – Cat# MBS820916

Immunofluorescence (IF) of anti-CHAT antibody

Immunofluorescent analysis of CHAT staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
Rabbit Anti-CHAT Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS820916

Other Notes

Small volumes of anti-CHAT antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CHAT. It may not necessarily be applicable to this product.

NCBI GI #
218931221
NCBI GeneID
1103
NCBI Accession #
NP_001136401.1
UniProt Primary Accession #
P28329
UniProt Secondary Accession #
Q16488; Q9BQ23; Q9BQ35; Q9BQE1; A2BDF4; A2BDF5
UniProt Related Accession #
P28329
GenBank Nucleotide #
NM_001142929.1
NCBI Official Full Name
choline O-acetyltransferase isoform 1
NCBI Official Synonym Full Names
choline O-acetyltransferase
NCBI Official Symbol
CHAT
NCBI Official Synonym Symbols
CMS1A; CMS1A2; CHOACTASE
UniProt Protein Name
Choline O-acetyltransferase
Protein Family
(Z)-3-hexen-1-ol acetyltransferase
UniProt Gene Name
CHAT
UniProt Synonym Gene Names
CHOACTase; ChAT; Choline acetylase
UniProt Entry Name
CLAT_HUMAN
NCBI Protein Information
choline O-acetyltransferase; choline acetylase; acetyl CoA:choline O-acetyltransferase

NCBI Summary for CHAT

This gene encodes an enzyme which catalyzes the biosynthesis of the neurotransmitter acetylcholine. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer's disease. Polymorphisms in this gene have been associated with Alzheimer's disease and mild cognitive impairment. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform. [provided by RefSeq, May 2010]
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UniProt Comments for CHAT

CHAT: an enzyme that catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses. Four alternative splice variants have been described.

Protein type: Lipid Metabolism - glycerophospholipid; Acetyltransferase; EC 2.3.1.6

Chromosomal Location of Human Ortholog: 10q11.2

Cellular Component: mitochondrion; cell soma; axon; cytoplasm; nucleus; cytosol

Molecular Function: choline O-acetyltransferase activity

Biological Process: synaptic transmission; rhythmic behavior; muscle development; rhythmic excitation; neurotransmitter secretion; phospholipid metabolic process; glycerophospholipid biosynthetic process; dendrite development; phosphatidylcholine biosynthetic process; neuromuscular synaptic transmission; establishment of synaptic specificity at neuromuscular junction; adult walking behavior; neurotransmitter biosynthetic process

Disease: Myasthenic Syndrome, Congenital, Associated With Episodic Apnea
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Research Articles on CHAT

1. The results demonstrate that human NSCs over-expressing ChAT improve cognitive function and physical activity of aging mice.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CHAT antibody

 Reference Product  PubMed Publications
 ACHE antibody  >878 publications with CHAT and ACHE
 NGF antibody  >591 publications with CHAT and NGF
 SLC18A3 antibody  >130 publications with CHAT and SLC18A3
 SLC5A7 antibody  >8 publications with CHAT and SLC5A7
 VAMP2 antibody  >1 publications with CHAT and VAMP2
 KIFAP3 antibody  >1 publications with CHAT and KIFAP3

Pathways associated with anti-CHAT antibody

 Products by Pathway  Pathway Diagram
 Acetylcholine Neurotransmitter Release Cycle Pathway antibodies  Acetylcholine Neurotransmitter Release Cycle Pathway Diagram
 Acetylcholine Synthesis Pathway antibodies  Acetylcholine Synthesis Pathway Diagram
 Biogenic Amine Synthesis Pathway antibodies  Biogenic Amine Synthesis Pathway Diagram
 Cholinergic Synapse Pathway antibodies  Cholinergic Synapse Pathway Diagram
 Glycerophospholipid Biosynthesis Pathway antibodies  Glycerophospholipid Biosynthesis Pathway Diagram
 Glycerophospholipid Metabolism Pathway antibodies  Glycerophospholipid Metabolism Pathway Diagram
 Glycerophospholipid Metabolism Pathway antibodies  Glycerophospholipid Metabolism Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Lipids And Lipoproteins Pathway antibodies  Metabolism Of Lipids And Lipoproteins Pathway Diagram
 Neuronal System Pathway antibodies  Neuronal System Pathway Diagram

Diseases associated with anti-CHAT antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >2505 publications with CHAT and Nervous System Diseases
 Brain Diseases Antibodies  >1345 publications with CHAT and Brain Diseases
 Neurodegenerative Diseases Antibodies  >1030 publications with CHAT and Neurodegenerative Diseases
 Neurobehavioral Manifestations Antibodies  >886 publications with CHAT and Neurobehavioral Manifestations
 Disease Models, Animal Antibodies  >544 publications with CHAT and Disease Models, Animal
 Nerve Degeneration Antibodies  >435 publications with CHAT and Nerve Degeneration
 Cardiovascular Diseases Antibodies  >313 publications with CHAT and Cardiovascular Diseases
 Movement Disorders Antibodies  >270 publications with CHAT and Movement Disorders
 Memory Disorders Antibodies  >262 publications with CHAT and Memory Disorders
 Cognition Disorders Antibodies  >245 publications with CHAT and Cognition Disorders

Organs/Tissues associated with anti-CHAT antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >5882 publications with CHAT and Brain
 Eye Antibodies  >551 publications with CHAT and Eye
 Testis Antibodies  >13 publications with CHAT and Testis
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