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P15 Ink4b (CDKN2B) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS821044

MBS821044 is an antibody targeting CDKN2B. Also known as Anti-p15 INK4b Antibody. Reactive in Human, Mouse, Rat, Monkey, Pig. Host: Rabbit. Rabbit polyclonal antibody to p15 INK4b More details

$225 / 0.03 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
p15 INK4b (CDKN2B), Polyclonal Antibody
Full Product Name
Anti-p15 INK4b Antibody
Product Gene Name
anti-CDKN2B antibody
Product Synonym Names
MTS2; Cyclin-dependent kinase 4 inhibitor B; Multiple tumor suppressor 2; MTS-2; p14-INK4b; p15-INK4b; p15INK4B
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat, Monkey, Pig
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Specificity
Recognizes endogenous levels of p15 INK4b protein.
Preparation and Storage
Upon receipt aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
Antibody/Peptide Pairs
p15 INK4b peptide (MBS823637) is used for blocking the activity of p15 INK4b antibody (MBS821044)
OMIM
600431
3D Structure
ModBase 3D Structure for P42772
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human p15 INK4b. The exact sequence is proprietary.
Applications Tested/Suitable for anti-CDKN2B antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence/Immunocytochemistry (IF/ICC), Immunoprecipitation (IP)
Application Notes for anti-CDKN2B antibody
WB: 1/500 - 1/1000
IH: 1/50 - 1/100
IF/IC: 1/50 - 1/200
IP: (1/10-1/100)

Western Blot (WB) of anti-CDKN2B antibody

Western blot analysis of p15 INK4b expression in HEK293T (A), Hela (B), mouse liver (C) whole cell lysates.
(Predicted band size: 14 kD; Observed band size: 15 kD)
Rabbit Anti-CDKN2B Polyclonal Antibody – Cat# MBS821044

Immunohistochemistry (IHC) of anti-CDKN2B antibody

Immunohistochemical analysis of p15 INK4b staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Rabbit Anti-CDKN2B Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS821044

Immunofluorescence (IF) of anti-CDKN2B antibody

Immunofluorescent analysis of p15 INK4b staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Rabbit Anti-CDKN2B Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS821044

Other Notes

Small volumes of anti-CDKN2B antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CDKN2B. It may not necessarily be applicable to this product.

NCBI GI #
17981694
NCBI GeneID
1030
NCBI Accession #
NP_004927.2
UniProt Primary Accession #
P42772
UniProt Secondary Accession #
O15125; Q6FI09
UniProt Related Accession #
P42772; AAB32712
GenBank Nucleotide #
NM_004936.3
NCBI Official Full Name
cyclin-dependent kinase 4 inhibitor B isoform 1
NCBI Official Synonym Full Names
cyclin-dependent kinase inhibitor 2B (p15, inhibits CDK4)
NCBI Official Symbol
CDKN2B
NCBI Official Synonym Symbols
P15; MTS2; TP15; CDK4I; INK4B; p15INK4b
UniProt Protein Name
Cyclin-dependent kinase 4 inhibitor B
UniProt Synonym Protein Names
Multiple tumor suppressor 2; MTS-2; p14-INK4b; p15-INK4b; p15INK4B
Protein Family
Cyclin-dependent kinase 4 inhibitor
UniProt Gene Name
CDKN2B
UniProt Synonym Gene Names
MTS2; MTS-2; p15INK4B
UniProt Entry Name
CDN2B_HUMAN
NCBI Protein Information
cyclin-dependent kinase 4 inhibitor B; MTS-2; p14-INK4b; p14_INK4B; p15-INK4b; p15_INK4B; CDK4B inhibitor; p14_CDK inhibitor; p15 CDK inhibitor; CDK inhibitory protein; multiple tumor suppressor 2; cyclin-dependent kinases 4 and 6 binding protein

NCBI Summary for CDKN2B

This gene lies adjacent to the tumor suppressor gene CDKN2A in a region that is frequently mutated and deleted in a wide variety of tumors. This gene encodes a cyclin-dependent kinase inhibitor, which forms a complex with CDK4 or CDK6, and prevents the activation of the CDK kinases, thus the encoded protein functions as a cell growth regulator that controls cell cycle G1 progression. The expression of this gene was found to be dramatically induced by TGF beta, which suggested its role in the TGF beta induced growth inhibition. Two alternatively spliced transcript variants of this gene, which encode distinct proteins, have been reported. [provided by RefSeq, Jul 2008]
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UniProt Comments for CDKN2B

p15-INK4B: a potent inhibitor that nteracts strongly with CDK4 and CDK6. Potential effector of TGF-beta induced cell cycle arrest.

Protein type: Inhibitor; Cell cycle regulation; Protein kinase, regulatory subunit; Tumor suppressor

Chromosomal Location of Human Ortholog: 9p21

Cellular Component: nucleoplasm; cytoplasm; cytosol; nucleus

Molecular Function: cyclin-dependent protein kinase inhibitor activity; protein binding; protein kinase binding

Biological Process: spleen development; transcription initiation from RNA polymerase II promoter; transcription, DNA-dependent; megakaryocyte differentiation; mitotic cell cycle checkpoint; positive regulation of transforming growth factor beta receptor signaling pathway; cellular response to nutrient; liver development; response to organic cyclic substance; negative regulation of cell proliferation; response to cytokine stimulus; transforming growth factor beta receptor signaling pathway; cellular response to extracellular stimulus; negative regulation of phosphorylation; positive regulation of epithelial cell differentiation; positive regulation of transcription from RNA polymerase II promoter; gene expression; regulation of cyclin-dependent protein kinase activity; mitotic cell cycle; G2/M transition of mitotic cell cycle; cell cycle arrest; aging; negative regulation of epithelial cell proliferation
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Research Articles on CDKN2B

1. Study demonstrated that the CDKN2A/CDKN2B gene may be nominally associated with the efficacy of glibenclamide, and that CDKN2A/CDKN2B is associated with beta cell function.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CDKN2B antibody

 Reference Product  PubMed Publications
 CDKN1A antibody  >134 publications with CDKN2B and CDKN1A
 CDK4 antibody  >121 publications with CDKN2B and CDK4
 CDKN1B antibody  >96 publications with CDKN2B and CDKN1B
 CDK6 antibody  >55 publications with CDKN2B and CDK6
 MYC antibody  >52 publications with CDKN2B and MYC
 CCND1 antibody  >51 publications with CDKN2B and CCND1
 SMAD4 antibody  >21 publications with CDKN2B and SMAD4
 SMAD2 antibody  >17 publications with CDKN2B and SMAD2
 ZBTB17 antibody  >8 publications with CDKN2B and ZBTB17
 SP1 antibody  >6 publications with CDKN2B and SP1

Pathways associated with anti-CDKN2B antibody

 Products by Pathway  Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cellular Senescence Pathway antibodies  Cellular Senescence Pathway Diagram
 Cellular Responses To Stress Pathway antibodies  Cellular Responses To Stress Pathway Diagram
 Cyclin D Associated Events In G1 Pathway antibodies  Cyclin D Associated Events In G1 Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 FoxO Signaling Pathway antibodies  FoxO Signaling Pathway Diagram
 G1 Phase Pathway antibodies  G1 Phase Pathway Diagram

Diseases associated with anti-CDKN2B antibody

 Disease Name  Pubmed Publications
 Lymphoma Antibodies  >157 publications with CDKN2B and Lymphoma
 Chromosome Aberrations Antibodies  >110 publications with CDKN2B and Chromosome Aberrations
 Cardiovascular Diseases Antibodies  >73 publications with CDKN2B and Cardiovascular Diseases
 Adenocarcinoma Antibodies  >67 publications with CDKN2B and Adenocarcinoma
 Glioma Antibodies  >64 publications with CDKN2B and Glioma
 Carcinoma, Squamous Cell Antibodies  >55 publications with CDKN2B and Carcinoma, Squamous Cell
 Myelodysplastic Syndromes Antibodies  >47 publications with CDKN2B and Myelodysplastic Syndromes
 Neoplasms, Experimental Antibodies  >46 publications with CDKN2B and Neoplasms, Experimental
 Cell Transformation, Neoplastic Antibodies  >45 publications with CDKN2B and Cell Transformation, Neoplastic
 Heart Diseases Antibodies  >39 publications with CDKN2B and Heart Diseases

Organs/Tissues associated with anti-CDKN2B antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >70 publications with CDKN2B and Brain
 Lung Antibodies  >56 publications with CDKN2B and Lung
 Liver Antibodies  >47 publications with CDKN2B and Liver
 Skin Antibodies  >35 publications with CDKN2B and Skin
 Muscle Antibodies  >28 publications with CDKN2B and Muscle
 Bladder Antibodies  >21 publications with CDKN2B and Bladder
 Prostate Antibodies  >20 publications with CDKN2B and Prostate
 Stomach Antibodies  >15 publications with CDKN2B and Stomach
 Embryonic Tissue Antibodies  >11 publications with CDKN2B and Embryonic Tissue
 Pancreas Antibodies  >11 publications with CDKN2B and Pancreas
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