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SF3B14 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS821067

MBS821067 is an antibody targeting SF3B14. Also known as Anti-SAP14 Antibody. Reactive in Human, Mouse, Rat, Bovine, Zebrafish. Host: Rabbit. Rabbit polyclonal antibody to SAP14 More details

$225 / 0.03 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
SAP14 (SF3B14), Polyclonal Antibody
Full Product Name
Anti-SAP14 Antibody
Product Gene Name
anti-SF3B14 antibody
Product Synonym Names
SAP14; SF3B14; SF3B14A; Splicing factor 3B subunit 6; Pre-mRNA branch site protein p14; SF3b 14 kDa subunit; SF3B14a; Spliceosome-associated protein, 14-kDa; Splicing factor 3b, subunit 6, 14kDa
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat, Bovine, Zebrafish
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Specificity
Recognizes endogenous levels of SAP14 protein.
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
Antibody/Peptide Pairs
SAP14 peptide (MBS823680) is used for blocking the activity of SAP14 antibody (MBS821067)
Molecular Weight
14,585 Da
OMIM
607835
3D Structure
ModBase 3D Structure for Q9Y3B4
Entrez Gene
51639 (Human); 66055 (Mouse)
SwissProt
Q9Y3B4 (Human); P59708 (Mouse)
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human SAP14. The exact sequence is proprietary.
Applications Tested/Suitable for anti-SF3B14 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemsitry (ICC)
Application Notes for anti-SF3B14 antibody
WB (1/500 - 1/1000), IF/ICC (1/100 - 1/500)

Western Blot (WB) of anti-SF3B14 antibody

Western blot analysis of SAP14 expression in HepG2 (A), RT4 (B) whole cell lysates.
Rabbit Anti-SF3B14 Polyclonal Antibody – Cat# MBS821067

Immunofluorescence (IF) of anti-SF3B14 antibody

Immunofluorescent analysis of SAP14 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Rabbit Anti-SF3B14 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS821067

Other Notes

Small volumes of anti-SF3B14 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for SF3B14. It may not necessarily be applicable to this product.

NCBI GI #
7706326
NCBI GeneID
51639
NCBI Accession #
NP_057131.1
UniProt Primary Accession #
Q9Y3B4
UniProt Secondary Accession #
Q53TM1
UniProt Related Accession #
Q9Y3B4
GenBank Nucleotide #
NM_016047.3
NCBI Official Full Name
splicing factor 3B subunit 6
NCBI Official Synonym Full Names
splicing factor 3b, subunit 6, 14kDa
NCBI Official Symbol
SF3B6
NCBI Official Synonym Symbols
P14; Ht006; SAP14; SAP14a; SF3B14; CGI-110; HSPC175; SF3B14a
UniProt Protein Name
Splicing factor 3B subunit 6
UniProt Synonym Protein Names
Pre-mRNA branch site protein p14; SF3b 14 kDa subunit; SF3B14a; Spliceosome-associated protein, 14-kDa; Splicing factor 3b, subunit 6, 14kDa
UniProt Gene Name
SF3B6
UniProt Synonym Gene Names
SAP14; SF3B14; SF3B14A; SF3B14a
UniProt Entry Name
SF3B6_HUMAN
NCBI Protein Information
splicing factor 3B subunit 6; SF3b 14 kDa subunit; pre mRNA branch site protein p14; pre-mRNA branch site protein p14; splicing factor 3B, 14 kDa subunit; spliceosome-associated protein, 14-kDa; spliceosome-associated protein, 14 kDa subunit

NCBI Summary for SF3B14

This gene encodes a 14 kDa protein subunit of the splicing factor 3b complex. Splicing factor 3b associates with both the U2 and U11/U12 small nuclear ribonucleoprotein complexes (U2 snRNP) of spliceosomes. This 14 kDa protein interacts directly with subunit 1 of the splicing factor 3b complex. This 14 kDa protein also interacts directly with the adenosine that carries out the first transesterification step of splicing at the pre-mRNA branch site. [provided by RefSeq, Jul 2008]
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UniProt Comments for SF3B14

SF3B6: subunit of the splicing factor SF3B. Necessary for the splicing of pre-mRNA. Directly contacts the pre-mRNA branch site adenosine for the first catalytic step of splicing. Enters the spliceosome and associates with the pre-mRNA branch site as part of the 17S U2 or, in the case of the minor spliceosome, as part of the 18S U11/U12 snRNP complex, and thus may facilitate the interaction of these snRNP with the branch sites of U2 and U12 respectively.

Protein type: Spliceosome; RNA splicing; RNA-binding

Chromosomal Location of Human Ortholog: 2p23

Cellular Component: nucleoplasm; U12-dependent spliceosome

Molecular Function: nucleotide binding

Biological Process: nuclear mRNA splicing, via spliceosome; RNA splicing; gene expression
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Research Articles on SF3B14

1. Observational study of gene-disease association. (HuGE Navigator)

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-SF3B14 antibody

 Products by Pathway  Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Processing Of Capped Intron-Containing Pre-mRNA Pathway antibodies  Processing Of Capped Intron-Containing Pre-mRNA Pathway Diagram
 Spliceosome Pathway antibodies  Spliceosome Pathway Diagram
 Spliceosome Pathway antibodies  Spliceosome Pathway Diagram
 Spliceosome, U2-snRNP Pathway antibodies  Spliceosome, U2-snRNP Pathway Diagram
 Spliceosome, U2-snRNP Pathway antibodies  Spliceosome, U2-snRNP Pathway Diagram
 MRNA Splicing Pathway antibodies  MRNA Splicing Pathway Diagram
 MRNA Splicing - Major Pathway antibodies  MRNA Splicing - Major Pathway Diagram
 MRNA Splicing - Minor Pathway antibodies  MRNA Splicing - Minor Pathway Diagram
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