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N4BP1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS821201

MBS821201 is an antibody targeting N4BP1. Reactive in Human. Host: Rabbit. Rabbit polyclonal antibody to N4BP1 More details

$225 / 0.03 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
N4BP1, Polyclonal Antibody
Full Product Name
Anti-N4BP1 Antibody
Product Gene Name
Product Synonym Names
KIAA0615; NEDD4-binding protein 1; N4BP1
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Specificity
Recognizes endogenous levels of N4BP1 protein.
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
Antibody/Peptide Pairs
N4BP1 peptide (MBS824056) is used for blocking the activity of N4BP1 antibody (MBS821201)
Molecular Weight
100,379 Da
3D Structure
ModBase 3D Structure for O75113
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human N4BP1. The exact sequence is proprietary.
Applications Tested/Suitable for anti-N4BP1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
Application Notes for anti-N4BP1 antibody
WB (1/500 - 1/1000), IH (1/100 - 1/200), IP (1/10 - 1/100)

Western Blot (WB) of anti-N4BP1 antibody

Western blot analysis of N4BP1 expression in HeLa (A), Jurkat (B) whole cell lysates.
Rabbit Anti-N4BP1 Polyclonal Antibody – Cat# MBS821201

Immunohistochemistry (IHC) of anti-N4BP1 antibody

Immunohistochemical analysis of N4BP1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Rabbit Anti-N4BP1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS821201

Other Notes

Small volumes of anti-N4BP1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for N4BP1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
NEDD4-binding protein 1
NCBI Official Synonym Full Names
NEDD4 binding protein 1
NCBI Official Symbol
N4BP1
UniProt Protein Name
NEDD4-binding protein 1
Protein Family
UniProt Gene Name
N4BP1
UniProt Synonym Gene Names
KIAA0615; N4BP1
UniProt Entry Name
N4BP1_HUMAN
NCBI Protein Information
NEDD4-binding protein 1

UniProt Comments for N4BP1

N4BP1: Inhibitor of the E3 ubiquitin-protein ligase ITCH. Acts by interacting with the second WW domain of ITCH, leading to compete with ITCH's substrates and impairing ubiquitination of substrates. Belongs to the N4BP1 family.

Protein type: Unknown function; Nucleolus

Chromosomal Location of Human Ortholog: 16q12.1

Cellular Component: PML body; nucleolus

Molecular Function: protein binding

Biological Process: negative regulation of protein ubiquitination; negative regulation of proteasomal ubiquitin-dependent protein catabolic process
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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