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C/EBP Delta/Epsilon (CEBPD) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS821238

MBS821238 is an antibody targeting CEBPD. Also known as Anti-C/EBP delta/epsilon Antibody. Reactive in Human, Mouse, Rat, Bovine, Sheep. Host: Rabbit. Rabbit polyclonal antibody to C/EBP delta/epsilon More details

$225 / 0.03 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
C/EBP delta/epsilon (CEBPD), Polyclonal Antibody
Full Product Name
Anti-C/EBP delta/epsilon Antibody
Product Gene Name
anti-CEBPD antibody
Product Synonym Gene Name
CEBPE
Product Synonym Names
CEBPD; CCAAT/enhancer-binding protein delta; C/EBP delta; Nuclear factor NF-IL6-beta; NF-IL6-beta; CEBPE; CCAAT/enhancer-binding protein epsilon; C/EBP epsilon
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat, Bovine, Sheep
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Specificity
Recognizes endogenous levels of C/EBP delta/epsilon protein.
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
Antibody/Peptide Pairs
C/EBP delta/epsilon peptide (MBS823951) is used for blocking the activity of C/EBP delta/epsilon antibody (MBS821238)
Molecular Weight
28,467 Da
OMIM
116898
3D Structure
ModBase 3D Structure for P49716
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human C/EBP delta/epsilon. The exact sequence is proprietary.
Applications Tested/Suitable for anti-CEBPD antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
Application Notes for anti-CEBPD antibody
WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/ICC (1/100 - 1/500)

Western Blot (WB) of anti-CEBPD antibody

Western blot analysis of C/EBP delta/epsilon expression in HepG2 (A) whole cell lysates.
Rabbit Anti-CEBPD Polyclonal Antibody – Cat# MBS821238

Immunohistochemistry (IHC) of anti-CEBPD antibody

Immunohistochemical analysis of C/EBP delta/epsilon staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Rabbit Anti-CEBPD Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS821238

Immunofluorescence (IF) of anti-CEBPD antibody

Immunofluorescent analysis of C/EBP delta/epsilon staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Rabbit Anti-CEBPD Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS821238

Other Notes

Small volumes of anti-CEBPD antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CEBPD. It may not necessarily be applicable to this product.

NCBI GI #
28872798
NCBI GeneID
1052
NCBI Accession #
NP_005186.2
UniProt Primary Accession #
P49716
UniProt Secondary Accession #
Q14937; Q2M2X9
UniProt Related Accession #
P49716
GenBank Nucleotide #
NM_005195.3
NCBI Official Full Name
CCAAT/enhancer-binding protein delta
NCBI Official Synonym Full Names
CCAAT/enhancer binding protein (C/EBP), delta
NCBI Official Symbol
CEBPD
NCBI Official Synonym Symbols
CELF; CRP3; C/EBP-delta; NF-IL6-beta
UniProt Protein Name
CCAAT/enhancer-binding protein delta
UniProt Synonym Protein Names
Nuclear factor NF-IL6-beta; NF-IL6-beta
Protein Family
CCAAT/enhancer-binding protein
UniProt Gene Name
CEBPD
UniProt Synonym Gene Names
C/EBP delta; NF-IL6-beta
UniProt Entry Name
CEBPD_HUMAN
NCBI Protein Information
CCAAT/enhancer-binding protein delta; c/EBP delta; nuclear factor NF-IL6-beta

NCBI Summary for CEBPD

The protein encoded by this intronless gene is a bZIP transcription factor which can bind as a homodimer to certain DNA regulatory regions. It can also form heterodimers with the related protein CEBP-alpha. The encoded protein is important in the regulation of genes involved in immune and inflammatory responses, and may be involved in the regulation of genes associated with activation and/or differentiation of macrophages. The cytogenetic location of this locus has been reported as both 8p11 and 8q11. [provided by RefSeq, Sep 2010]
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UniProt Comments for CEBPD

C/EBP-delta: C/EBP is a DNA-binding protein that recognizes two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers. Important transcriptional activator in the regulation of genes involved in immune and inflammatory responses, may play an important role in the regulation of the several genes associated with activation and/or differentiation of macrophages. Binds DNA as a dimer and can form stable heterodimers with C/EBP alpha. Interacts with SPI1/PU.1. Interacts with PRDM16. Belongs to the bZIP family. C/EBP subfamily.

Protein type: Motility/polarity/chemotaxis; DNA-binding

Chromosomal Location of Human Ortholog: 8p11.2-p11.1

Cellular Component: nucleus

Biological Process: transcription from RNA polymerase II promoter; positive regulation of transcription from RNA polymerase II promoter
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Research Articles on CEBPD

1. GSK3beta-mediated CEBPD phosphorylation in astrocytes plays an important role in the activation of microglia.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CEBPD antibody

 Reference Product  PubMed Publications
 CEBPB antibody  >23 publications with CEBPD and CEBPB
 STAT3 antibody  >10 publications with CEBPD and STAT3
 MYC antibody  >5 publications with CEBPD and MYC
 RELA antibody  >4 publications with CEBPD and RELA
 CREBBP antibody  >2 publications with CEBPD and CREBBP
 RXRA antibody  >1 publications with CEBPD and RXRA

Pathways associated with anti-CEBPD antibody

 Products by Pathway  Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 C-MYB Transcription Factor Network Pathway antibodies  C-MYB Transcription Factor Network Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 FOXA2 And FOXA3 Transcription Factor Networks Pathway antibodies  FOXA2 And FOXA3 Transcription Factor Networks Pathway Diagram
 IL17 Signaling Pathway antibodies  IL17 Signaling Pathway Diagram
 IL6-mediated Signaling Events Pathway antibodies  IL6-mediated Signaling Events Pathway Diagram
 Regulation Of Retinoblastoma Protein Pathway antibodies  Regulation Of Retinoblastoma Protein Pathway Diagram
 Transcriptional Regulation Of White Adipocyte Differentiation Pathway antibodies  Transcriptional Regulation Of White Adipocyte Differentiation Pathway Diagram
 Validated Targets Of C-MYC Transcriptional Repression Pathway antibodies  Validated Targets Of C-MYC Transcriptional Repression Pathway Diagram

Diseases associated with anti-CEBPD antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >38 publications with CEBPD and Inflammation
 Necrosis Antibodies  >20 publications with CEBPD and Necrosis
 Nervous System Diseases Antibodies  >14 publications with CEBPD and Nervous System Diseases
 Breast Neoplasms Antibodies  >12 publications with CEBPD and Breast Neoplasms
 Brain Diseases Antibodies  >9 publications with CEBPD and Brain Diseases
 Disease Models, Animal Antibodies  >9 publications with CEBPD and Disease Models, Animal
 Liver Diseases Antibodies  >9 publications with CEBPD and Liver Diseases
 Liver Neoplasms Antibodies  >8 publications with CEBPD and Liver Neoplasms
 Adenocarcinoma Antibodies  >8 publications with CEBPD and Adenocarcinoma
 Cardiovascular Diseases Antibodies  >7 publications with CEBPD and Cardiovascular Diseases

Organs/Tissues associated with anti-CEBPD antibody

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >29 publications with CEBPD and Muscle
 Liver Antibodies  >24 publications with CEBPD and Liver
 Vascular Antibodies  >20 publications with CEBPD and Vascular
 Bone Antibodies  >19 publications with CEBPD and Bone
 Lung Antibodies  >17 publications with CEBPD and Lung
 Brain Antibodies  >15 publications with CEBPD and Brain
 Heart Antibodies  >13 publications with CEBPD and Heart
 Bone Marrow Antibodies  >10 publications with CEBPD and Bone Marrow
 Mammary Gland Antibodies  >8 publications with CEBPD and Mammary Gland
 Connective Tissue Antibodies  >7 publications with CEBPD and Connective Tissue
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