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ANAPC1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS821277

MBS821277 is an antibody targeting ANAPC1. Reactive in Human, Mouse, Rat. Host: Rabbit. Rabbit polyclonal antibody to ANAPC1 More details

$225 / 0.03 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
ANAPC1, Polyclonal Antibody
Full Product Name
Anti-ANAPC1 Antibody
Product Gene Name
anti-ANAPC1 antibody
Product Synonym Names
TSG24; Anaphase-promoting complex subunit 1; APC1; Cyclosome subunit 1; Mitotic checkpoint regulator; Testis-specific gene 24 protein
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Specificity
Recognizes endogenous levels of ANAPC1 protein.
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
Antibody/Peptide Pairs
ANAPC1 peptide (MBS823569) is used for blocking the activity of ANAPC1 antibody (MBS821277)
Molecular Weight
216,500 Da
OMIM
608473
3D Structure
ModBase 3D Structure for Q9H1A4
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human ANAPC1. The exact sequence is proprietary.
Applications Tested/Suitable for anti-ANAPC1 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemsitry (ICC)
Application Notes for anti-ANAPC1 antibody
WB (1/500 - 1/1000), IF/ICC (1/100 - 1/500)

Western Blot (WB) of anti-ANAPC1 antibody

Western blot analysis of ANAPC1 expression in HeLa (A) whole cell lysates.
Rabbit Anti-ANAPC1 Polyclonal Antibody – Cat# MBS821277

Immunofluorescence (IF) of anti-ANAPC1 antibody

Immunofluorescent analysis of ANAPC1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Rabbit Anti-ANAPC1 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS821277

Other Notes

Small volumes of anti-ANAPC1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ANAPC1. It may not necessarily be applicable to this product.

NCBI GI #
12056971
NCBI GeneID
64682
NCBI Accession #
NP_073153.1
UniProt Primary Accession #
Q9H1A4
UniProt Secondary Accession #
Q2M3H8; Q9BSE6; Q9H8D0
UniProt Related Accession #
Q9H1A4
GenBank Nucleotide #
NM_022662.3
NCBI Official Full Name
anaphase-promoting complex subunit 1
NCBI Official Synonym Full Names
anaphase promoting complex subunit 1
NCBI Official Symbol
ANAPC1
NCBI Official Synonym Symbols
APC1; MCPR; TSG24
UniProt Protein Name
Anaphase-promoting complex subunit 1
UniProt Synonym Protein Names
Cyclosome subunit 1; Mitotic checkpoint regulator; Testis-specific gene 24 protein
UniProt Gene Name
ANAPC1
UniProt Synonym Gene Names
TSG24; APC1
UniProt Entry Name
APC1_HUMAN
NCBI Protein Information
anaphase-promoting complex subunit 1; cyclosome subunit 1; mitotic checkpoint regulator; testis-specific gene 24 protein; anaphase-promoting complex 1 (meiotic checkpoint regulator)

NCBI Summary for ANAPC1

This gene encodes a subunit of the anaphase-promoting complex. This complex is an E3 ubiquitin ligase that regulates progression through the metaphase to anaphase portion of the cell cycle by ubiquitinating proteins which targets them for degradation. [provided by RefSeq, Dec 2011]

UniProt Comments for ANAPC1

APC1: Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. Belongs to the APC1 family.

Protein type: Cell cycle regulation; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 2q12.1

Cellular Component: nucleoplasm; anaphase-promoting complex; cytosol

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; mitosis; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; cell division; mitotic cell cycle spindle assembly checkpoint; mitotic cell cycle
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Research Articles on ANAPC1

1. Data indicate that regulation of Rad17 turnover is through the Cdh1/anaphase-promoting complex pathway in breast cancer cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ANAPC1 antibody

 Reference Product  PubMed Publications
 ANAPC2 antibody  >8 publications with ANAPC1 and ANAPC2
 CDC16 antibody  >6 publications with ANAPC1 and CDC16
 CDC23 antibody  >6 publications with ANAPC1 and CDC23
 ANAPC4 antibody  >6 publications with ANAPC1 and ANAPC4
 CDC27 antibody  >6 publications with ANAPC1 and CDC27
 ANAPC5 antibody  >5 publications with ANAPC1 and ANAPC5
 ANAPC7 antibody  >3 publications with ANAPC1 and ANAPC7
 CDC20 antibody  >3 publications with ANAPC1 and CDC20
 PTTG1 antibody  >1 publications with ANAPC1 and PTTG1
 CCNB1 antibody  >1 publications with ANAPC1 and CCNB1

Pathways associated with anti-ANAPC1 antibody

 Products by Pathway  Pathway Diagram
 APC/C Complex Pathway antibodies  APC/C Complex Pathway Diagram
 APC/C Complex Pathway antibodies  APC/C Complex Pathway Diagram
 APC/C-mediated Degradation Of Cell Cycle Proteins Pathway antibodies  APC/C-mediated Degradation Of Cell Cycle Proteins Pathway Diagram
 APC/C:Cdc20 Mediated Degradation Of Cyclin B Pathway antibodies  APC/C:Cdc20 Mediated Degradation Of Cyclin B Pathway Diagram
 APC/C:Cdc20 Mediated Degradation Of Securin Pathway antibodies  APC/C:Cdc20 Mediated Degradation Of Securin Pathway Diagram
 APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway antibodies  APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway Diagram
 APC/C:Cdh1 Mediated Degradation Of Cdc20 And Other APC/C:Cdh1 Targeted Proteins In Late Mitosis/early G1 Pathway antibodies  APC/C:Cdh1 Mediated Degradation Of Cdc20 And Other APC/C:Cdh1 Targeted Proteins In Late Mitosis/early G1 Pathway Diagram
 Activation Of APC/C And APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway antibodies  Activation Of APC/C And APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Antigen Processing: Ubiquitination & Proteasome Degradation Pathway antibodies  Antigen Processing: Ubiquitination & Proteasome Degradation Pathway Diagram

Diseases associated with anti-ANAPC1 antibody

 Disease Name  Pubmed Publications
 Adenocarcinoma Antibodies  >1 publications with ANAPC1 and Adenocarcinoma
 Hyperplasia Antibodies  >1 publications with ANAPC1 and Hyperplasia

Organs/Tissues associated with anti-ANAPC1 antibody

 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >2 publications with ANAPC1 and Lung
 Skin Antibodies  >1 publications with ANAPC1 and Skin
 Kidney Antibodies  >1 publications with ANAPC1 and Kidney
 Uterus Antibodies  >1 publications with ANAPC1 and Uterus
 Stomach Antibodies  >1 publications with ANAPC1 and Stomach
 Intestine Antibodies  >1 publications with ANAPC1 and Intestine
 Brain Antibodies  >1 publications with ANAPC1 and Brain
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