DNA Polymerase Alpha 1 (POLA1) Antibody – Rabbit Polyclonal

POLA: Plays an essential role in the initiation of DNA replication. During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1/p180, a regulatory subunit POLA2/p70 and two primase subunits PRIM1/p49 and PRIM2/p58) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1. The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands. These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively. The reason this transfer occurs is because the polymerase alpha has limited processivity and lacks intrinsic 3' exonuclease activity for proofreading error, and therefore is not well suited for replicating long complexes. Belongs to the DNA polymerase type-B family.

Protein type: Nucleotide Metabolism - purine; DNA replication; Nucleotide Metabolism - pyrimidine; DNA repair, damage; EC 2.7.7.7; Transferase; Nuclear envelope

Chromosomal Location of Human Ortholog: Xp22.1-p21.3

Cellular Component: nucleoplasm; alpha DNA polymerase:primase complex; nuclear matrix; cytoplasm; nucleolus; nuclear envelope; nucleus; chromatin

Molecular Function: protein binding; DNA binding; DNA primase activity; protein heterodimerization activity; 4 iron, 4 sulfur cluster binding; metal ion binding; nucleoside binding; DNA-directed DNA polymerase activity; nucleotide binding; chromatin binding; protein kinase binding

Biological Process: DNA replication initiation; viral reproduction; DNA replication, synthesis of RNA primer; DNA strand elongation during DNA replication; DNA repair; leading strand elongation; lagging strand elongation; double-strand break repair via nonhomologous end joining; telomere maintenance via semi-conservative replication; G1/S-specific transcription in mitotic cell cycle; cell proliferation; DNA synthesis during DNA repair; telomere maintenance via recombination; mitotic cell cycle; DNA replication; telomere maintenance; G1/S transition of mitotic cell cycle

Disease: N Syndrome