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PMEPA1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS821500

MBS821500 is an antibody targeting PMEPA1. Reactive in Human. Host: Rabbit. Rabbit polyclonal antibody to PMEPA1 More details

$225 / 0.03 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PMEPA1, Polyclonal Antibody
Full Product Name
Anti-PMEPA1 Antibody
Product Gene Name
Product Synonym Names
STAG1; TMEPAI; Transmembrane prostate androgen-induced protein; Solid tumor-associated 1 protein
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Specificity
Recognizes endogenous levels of PMEPA1 protein.
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
Antibody/Peptide Pairs
PMEPA1 peptide (MBS824023) is used for blocking the activity of PMEPA1 antibody (MBS821500)
Molecular Weight
26,201 Da
OMIM
606564
3D Structure
ModBase 3D Structure for Q969W9
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PMEPA1. The exact sequence is proprietary.
Applications Tested/Suitable for anti-PMEPA1 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-PMEPA1 antibody
WB (1/500 - 1/1000), IH (1/100 - 1/200)

Western Blot (WB) of anti-PMEPA1 antibody

Western blot analysis of PMEPA1 expression in PC3 (A) whole cell lysates.
Rabbit Anti-PMEPA1 Polyclonal Antibody – Cat# MBS821500

Immunohistochemistry (IHC) of anti-PMEPA1 antibody

Immunohistochemical analysis of PMEPA1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Rabbit Anti-PMEPA1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS821500

Other Notes

Small volumes of anti-PMEPA1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PMEPA1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
protein TMEPAI isoform d
NCBI Official Synonym Full Names
prostate transmembrane protein, androgen induced 1
NCBI Official Symbol
PMEPA1
NCBI Official Synonym Symbols
STAG1; TMEPAI
UniProt Protein Name
Protein TMEPAI
UniProt Synonym Protein Names
Prostate transmembrane protein androgen induced 1; Solid tumor-associated 1 protein; Transmembrane prostate androgen-induced protein
Protein Family
UniProt Gene Name
PMEPA1
UniProt Synonym Gene Names
STAG1; TMEPAI
UniProt Entry Name
PMEPA_HUMAN
NCBI Protein Information
protein TMEPAI; solid tumor-associated 1 protein; transmembrane, prostate androgen induced RNA; prostate transmembrane protein androgen induced 1

NCBI Summary for PMEPA1

This gene encodes a transmembrane protein that contains a Smad interacting motif (SIM). Expression of this gene is induced by androgens and transforming growth factor beta, and the encoded protein suppresses the androgen receptor and transforming growth factor beta signaling pathways though interactions with Smad proteins. Overexpression of this gene may play a role in multiple types of cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011]
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UniProt Comments for PMEPA1

TMEPAI: Involved in down-regulation of the androgen receptor (AR). Enhances ubiquitination and proteasome-mediated degradation of AR, probably by recruiting NEDD4. Belongs to the PMEPA1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 20q13.31-q13.33

Cellular Component: Golgi membrane; early endosome membrane; integral to membrane; plasma membrane; endosome membrane

Molecular Function: protein binding; WW domain binding

Biological Process: transforming growth factor beta receptor signaling pathway; androgen receptor signaling pathway; negative regulation of transforming growth factor beta receptor signaling pathway
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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