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RPL28 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS821512

MBS821512 is an antibody targeting RPL28. Reactive in Human, Monkey, Mouse, Rat. Host: Rabbit. Rabbit polyclonal antibody to RPL28 More details

$225 / 0.03 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
RPL28, Polyclonal Antibody
Full Product Name
Anti-RPL28 Antibody
Product Gene Name
anti-RPL28 antibody
Product Synonym Names
60S ribosomal protein L28
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Monkey, Mouse, Rat
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Specificity
Recognizes endogenous levels of RPL28 protein.
Preparation and Storage
Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
Antibody/Peptide Pairs
RPL28 peptide (MBS823824) is used for blocking the activity of RPL28 antibody (MBS821512)
OMIM
603638
3D Structure
ModBase 3D Structure for P46779
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human RPL28. The exact sequence is proprietary.
Applications Tested/Suitable for anti-RPL28 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-RPL28 antibody
WB (1/500 - 1/1000), IHC (1/50 - 1/100)

Western Blot (WB) of anti-RPL28 antibody

Western blot analysis of RPL28 expression in HeLa (A), MCF7 (B), HEK293 (C) whole cell lysates.
Rabbit Anti-RPL28 Polyclonal Antibody – Cat# MBS821512

Immunohistochemistry (IHC) of anti-RPL28 antibody

Immunohistochemical analysis of RPL28 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Rabbit Anti-RPL28 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS821512

Western Blot (WB) of anti-RPL28 antibody

Western blot analysis of RPL28 expression in HEK293T (A), Hela (B), H446 (C), mouse liver (D), mouse kidney (E), rat liver (F), rat kidney (G) whole cell lysates. (Predicted band size: 15 kD; Observed band size: 16 kD)
Rabbit Anti-RPL28 Polyclonal Antibody, Western Blot (WB) – Cat# MBS821512

Other Notes

Small volumes of anti-RPL28 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for RPL28. It may not necessarily be applicable to this product.

NCBI GI #
13904866
NCBI GeneID
6158
NCBI Accession #
NP_000982.2
UniProt Primary Accession #
P46779
UniProt Secondary Accession #
Q6IAY0; Q96FX1; Q9BWQ0; B2R4A6; B4DEP9; C9JB50; E9PB24; G5E9L2
UniProt Related Accession #
P46779
GenBank Nucleotide #
NM_000991.4
NCBI Official Full Name
60S ribosomal protein L28 isoform 2
NCBI Official Synonym Full Names
ribosomal protein L28
NCBI Official Symbol
RPL28
NCBI Official Synonym Symbols
L28
UniProt Protein Name
60S ribosomal protein L28
Protein Family
50S ribosomal protein
UniProt Gene Name
RPL28
UniProt Entry Name
RL28_HUMAN
NCBI Protein Information
60S ribosomal protein L28

NCBI Summary for RPL28

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L28E family of ribosomal proteins. It is located in the cytoplasm. Variable expression of this gene in colorectal cancers compared to adjacent normal tissues has been observed, although no correlation between the level of expression and the severity of the disease has been found. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Oct 2008]
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UniProt Comments for RPL28

RPL28: a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L28E family of ribosomal proteins. It is located in the cytoplasm. Variable expression of this gene in colorectal cancers compared to adjacent normal tissues has been observed, although no correlation between the level of expression and the severity of the disease has been found. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Oct 2008]

Protein type: Ribosomal; Translation

Chromosomal Location of Human Ortholog: 19q13.4

Cellular Component: membrane; cytoplasm; dendrite; cytosol

Molecular Function: protein binding; structural constituent of ribosome; RNA binding

Biological Process: SRP-dependent cotranslational protein targeting to membrane; cellular protein metabolic process; translational elongation; viral reproduction; translation; translational initiation; mRNA catabolic process, nonsense-mediated decay; gene expression; viral transcription; viral infectious cycle; translational termination
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Research Articles on RPL28

1. Observational study of gene-disease association. (HuGE Navigator)

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-RPL28 antibody

 Reference Product  PubMed Publications
 RPL5 antibody  >1 publications with RPL28 and RPL5
 RPL27A antibody  >1 publications with RPL28 and RPL27A
 RPS10 antibody  >1 publications with RPL28 and RPS10

Pathways associated with anti-RPL28 antibody

 Products by Pathway  Pathway Diagram
 Cap-dependent Translation Initiation Pathway antibodies  Cap-dependent Translation Initiation Pathway Diagram
 Cytoplasmic Ribosomal Proteins Pathway antibodies  Cytoplasmic Ribosomal Proteins Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Eukaryotic Translation Elongation Pathway antibodies  Eukaryotic Translation Elongation Pathway Diagram
 Eukaryotic Translation Initiation Pathway antibodies  Eukaryotic Translation Initiation Pathway Diagram
 Eukaryotic Translation Termination Pathway antibodies  Eukaryotic Translation Termination Pathway Diagram
 Formation Of A Pool Of Free 40S Subunits Pathway antibodies  Formation Of A Pool Of Free 40S Subunits Pathway Diagram
 GTP Hydrolysis And Joining Of The 60S Ribosomal Subunit Pathway antibodies  GTP Hydrolysis And Joining Of The 60S Ribosomal Subunit Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Influenza Infection Pathway antibodies  Influenza Infection Pathway Diagram

Diseases associated with anti-RPL28 antibody

 Disease Name  Pubmed Publications
 Cell Transformation, Neoplastic Antibodies  >1 publications with RPL28 and Cell Transformation, Neoplastic
 Pancreatic Neoplasms Antibodies  >1 publications with RPL28 and Pancreatic Neoplasms

Organs/Tissues associated with anti-RPL28 antibody

 Organ/Tissue Name  Pubmed Publications
 Esophagus Antibodies  >1 publications with RPL28 and Esophagus
 Placenta Antibodies  >1 publications with RPL28 and Placenta
 Liver Antibodies  >1 publications with RPL28 and Liver
 Testis Antibodies  >1 publications with RPL28 and Testis
 Intestine Antibodies  >1 publications with RPL28 and Intestine
 Embryonic Tissue Antibodies  >1 publications with RPL28 and Embryonic Tissue
 Brain Antibodies  >1 publications with RPL28 and Brain
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