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NEMF Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS821574

MBS821574 is an antibody targeting NEMF. Reactive in Human, Mouse, Monkey. Host: Rabbit. Rabbit polyclonal antibody to NEMF More details

$225 / 0.03 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
NEMF, Polyclonal Antibody
Full Product Name
Anti-NEMF Antibody
Product Gene Name
Product Synonym Names
SDCCAG1; Nuclear export mediator factor NEMF; Antigen NY-CO-1; Serologically defined colon cancer antigen 1
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Monkey
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Specificity
Recognizes endogenous levels of NEMF protein.
Preparation and Storage
Upon receipt aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
Antibody/Peptide Pairs
NEMF peptide (MBS823328) is used for blocking the activity of NEMF antibody (MBS821574)
OMIM
608378
3D Structure
ModBase 3D Structure for O60524
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human NEMF. The exact sequence is proprietary.
Applications Tested/Suitable for anti-NEMF antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
Application Notes for anti-NEMF antibody
WB (1/500 - 1/1000)
IHC (1/50 - 1/100)
IF/ICC (1/50-1/200)

Western Blot (WB) of anti-NEMF antibody

Western blot analysis of NEMF expression in Jurkat (A) whole cell lysates.
Rabbit Anti-NEMF Polyclonal Antibody – Cat# MBS821574

Immunohistochemistry (IHC) of anti-NEMF antibody

Immunohistochemical analysis of NEMF staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Rabbit Anti-NEMF Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS821574

Immunofluorescence (IF) of anti-NEMF antibody

Immunofluorescent analysis of NEMF staining in A2780 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin-AF594 was used to stain the cytoplasm (red). DAPI was used to stain the cell nuclei (blue).
Rabbit Anti-NEMF Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS821574

Other Notes

Small volumes of anti-NEMF antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for NEMF. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
Nuclear export mediator factor NEMF
NCBI Official Synonym Full Names
nuclear export mediator factor
NCBI Official Symbol
NEMF
NCBI Official Synonym Symbols
NY-CO-1; SDCCAG1
UniProt Protein Name
Nuclear export mediator factor NEMF
UniProt Synonym Protein Names
Antigen NY-CO-1; Serologically defined colon cancer antigen 1
UniProt Gene Name
NEMF
UniProt Synonym Gene Names
SDCCAG1
UniProt Entry Name
NEMF_HUMAN
NCBI Protein Information
nuclear export mediator factor NEMF; antigen NY-CO-1; serologically defined colon cancer antigen 1

UniProt Comments for NEMF

SDCCAG1: Plays a role in nuclear export. Belongs to the NEMF family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear export

Chromosomal Location of Human Ortholog: 14q22

Cellular Component: nucleus

Biological Process: nuclear export

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Organs/Tissues associated with anti-NEMF antibody

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