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Cytochrome B5a (CYB5A) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS821588

MBS821588 is an antibody targeting CYB5A. Also known as Anti-Cytochrome b5A Antibody. Reactive in Human. Host: Rabbit. Rabbit polyclonal antibody to Cytochrome b5A More details

$225 / 0.03 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Cytochrome b5A (CYB5A), Polyclonal Antibody
Full Product Name
Anti-Cytochrome b5A Antibody
Product Gene Name
anti-CYB5A antibody
Product Synonym Names
CYB5; Cytochrome b5; Microsomal cytochrome b5 type A; MCB5
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Specificity
Recognizes endogenous levels of Cytochrome b5A protein.
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
Antibody/Peptide Pairs
Cytochrome b5A peptide (MBS822673) is used for blocking the activity of Cytochrome b5A antibody (MBS821588)
Molecular Weight
14,169 Da
OMIM
phenotype 613218
3D Structure
ModBase 3D Structure for P00167
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Cytochrome b5A. The exact sequence is proprietary.
Applications Tested/Suitable for anti-CYB5A antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
Application Notes for anti-CYB5A antibody
WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/ICC (1/100 - 1/500)

Western Blot (WB) of anti-CYB5A antibody

Western blot analysis of Cytochrome b5A expression in HepG2 (A), HeLa (B) whole cell lysates.
Rabbit Anti-CYB5A Polyclonal Antibody – Cat# MBS821588

Immunohistochemistry (IHC) of anti-CYB5A antibody

Immunohistochemical analysis of Cytochrome b5A staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. AEC was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Rabbit Anti-CYB5A Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS821588

Immunofluorescence (IF) of anti-CYB5A antibody

Immunofluorescent analysis of Cytochrome b5A staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Rabbit Anti-CYB5A Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS821588

Other Notes

Small volumes of anti-CYB5A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CYB5A. It may not necessarily be applicable to this product.

NCBI GI #
299829246
NCBI GeneID
1528
NCBI Accession #
NP_001177736.1
UniProt Primary Accession #
P00167
UniProt Secondary Accession #
Q6IB14; A8MV91; F8WEU4
UniProt Related Accession #
P00167
GenBank Nucleotide #
NM_001190807.2
NCBI Official Full Name
cytochrome b5 isoform 3
NCBI Official Synonym Full Names
cytochrome b5 type A (microsomal)
NCBI Official Symbol
CYB5A
NCBI Official Synonym Symbols
CYB5; MCB5
UniProt Protein Name
Cytochrome b5
UniProt Synonym Protein Names
Microsomal cytochrome b5 type A; MCB5
Protein Family
Cytochrome
UniProt Gene Name
CYB5A
UniProt Synonym Gene Names
CYB5; MCB5
UniProt Entry Name
CYB5_HUMAN
NCBI Protein Information
cytochrome b5; type 1 cyt-b5

NCBI Summary for CYB5A

The protein encoded by this gene is a membrane-bound cytochrome that reduces ferric hemoglobin (methemoglobin) to ferrous hemoglobin, which is required for stearyl-CoA-desaturase activity. Defects in this gene are a cause of type IV hereditary methemoglobinemia. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]
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UniProt Comments for CYB5A

CYB5A: Cytochrome b5 is a membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases. Defects in CYB5A are the cause of methemoglobinemia CYB5A-related (METHB-CYB5A). A form of methemoglobinemia, a hematologic disease characterized by the presence of excessive amounts of methemoglobin in blood cells, resulting in decreased oxygen carrying capacity of the blood, cyanosis and hypoxia. Belongs to the cytochrome b5 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 18q23

Cellular Component: mitochondrial outer membrane; endoplasmic reticulum membrane; membrane; integral to membrane

Molecular Function: cytochrome-c oxidase activity; enzyme binding; metal ion binding; aldo-keto reductase activity; heme binding

Biological Process: vitamin metabolic process; response to cadmium ion; L-ascorbic acid metabolic process; water-soluble vitamin metabolic process

Disease: Methemoglobinemia Type Iv
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Research Articles on CYB5A

1. Cytochrome b5 seems to play the key role in the formation of lipid-radical cycles in membranes.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CYB5A antibody

 Reference Product  PubMed Publications
 CYB5R3 antibody  >8 publications with CYB5A and CYB5R3
 CYP1A1 antibody  >3 publications with CYB5A and CYP1A1
 POR antibody  >3 publications with CYB5A and POR
 CYP3A4 antibody  >2 publications with CYB5A and CYP3A4

Pathways associated with anti-CYB5A antibody

 Products by Pathway  Pathway Diagram
 Defective AMN Causes Hereditary Megaloblastic Anemia 1 Pathway antibodies  Defective AMN Causes Hereditary Megaloblastic Anemia 1 Pathway Diagram
 Defective BTD Causes Biotidinase Deficiency Pathway antibodies  Defective BTD Causes Biotidinase Deficiency Pathway Diagram
 Defective CD320 Causes Methylmalonic Aciduria Pathway antibodies  Defective CD320 Causes Methylmalonic Aciduria Pathway Diagram
 Defective CUBN Causes Hereditary Megaloblastic Anemia 1 Pathway antibodies  Defective CUBN Causes Hereditary Megaloblastic Anemia 1 Pathway Diagram
 Defective GIF Causes Intrinsic Factor Deficiency Pathway antibodies  Defective GIF Causes Intrinsic Factor Deficiency Pathway Diagram
 Defective HLCS Causes Multiple Carboxylase Deficiency Pathway antibodies  Defective HLCS Causes Multiple Carboxylase Deficiency Pathway Diagram
 Defective LMBRD1 Causes Methylmalonic Aciduria And Homocystinuria Type CblF Pathway antibodies  Defective LMBRD1 Causes Methylmalonic Aciduria And Homocystinuria Type CblF Pathway Diagram
 Defective MMAA Causes Methylmalonic Aciduria Type CblA Pathway antibodies  Defective MMAA Causes Methylmalonic Aciduria Type CblA Pathway Diagram
 Defective MMAB Causes Methylmalonic Aciduria Type CblB Pathway antibodies  Defective MMAB Causes Methylmalonic Aciduria Type CblB Pathway Diagram
 Defective MMACHC Causes Methylmalonic Aciduria And Homocystinuria Type CblC Pathway antibodies  Defective MMACHC Causes Methylmalonic Aciduria And Homocystinuria Type CblC Pathway Diagram

Diseases associated with anti-CYB5A antibody

 Disease Name  Pubmed Publications
 Drug-Related Side Effects and Adverse Reactions Antibodies  >2 publications with CYB5A and Drug-Related Side Effects and Adverse Reactions
 Breast Neoplasms Antibodies  >2 publications with CYB5A and Breast Neoplasms
 Hyperplasia Antibodies  >2 publications with CYB5A and Hyperplasia
 Adenocarcinoma Antibodies  >1 publications with CYB5A and Adenocarcinoma
 Weight Gain Antibodies  >1 publications with CYB5A and Weight Gain
 Carcinoma, Hepatocellular Antibodies  >1 publications with CYB5A and Carcinoma, Hepatocellular
 Liver Diseases Antibodies  >1 publications with CYB5A and Liver Diseases
 Prenatal Exposure Delayed Effects Antibodies  >1 publications with CYB5A and Prenatal Exposure Delayed Effects
 Kidney Diseases Antibodies  >1 publications with CYB5A and Kidney Diseases
 Neoplasm Metastasis Antibodies  >1 publications with CYB5A and Neoplasm Metastasis

Organs/Tissues associated with anti-CYB5A antibody

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >6 publications with CYB5A and Liver
 Testis Antibodies  >5 publications with CYB5A and Testis
 Adrenal Gland Antibodies  >3 publications with CYB5A and Adrenal Gland
 Kidney Antibodies  >3 publications with CYB5A and Kidney
 Embryonic Tissue Antibodies  >2 publications with CYB5A and Embryonic Tissue
 Blood Antibodies  >2 publications with CYB5A and Blood
 Placenta Antibodies  >1 publications with CYB5A and Placenta
 Intestine Antibodies  >1 publications with CYB5A and Intestine
 Bladder Antibodies  >1 publications with CYB5A and Bladder
 Prostate Antibodies  >1 publications with CYB5A and Prostate
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