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PPP2R1B Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS821756

MBS821756 is an antibody targeting PPP2R1B. Reactive in Human, Mouse, Rat, Monkey, Pig. Host: Rabbit. Rabbit polyclonal antibody to PPP2R1B More details

$225 / 0.03 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PPP2R1B, Polyclonal Antibody
Full Product Name
Anti-PPP2R1B Antibody
Product Gene Name
Product Synonym Names
Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A beta isoform; PP2A subunit A isoform PR65-beta; PP2A subunit A isoform R1-beta
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat, Monkey, Pig
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Specificity
Recognizes endogenous levels of PPP2R1B protein.
Preparation and Storage
Ships on blue ice. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
Antibody/Peptide Pairs
PPP2R1B peptide (MBS824233) is used for blocking the activity of PPP2R1B antibody (MBS821756)
OMIM
603113
3D Structure
ModBase 3D Structure for P30154
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human PPP2R1B. The exact sequence is proprietary.
Applications Tested/Suitable for anti-PPP2R1B antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemsitry (ICC)
Application Notes for anti-PPP2R1B antibody
WB (1/500 - 1/1000), IF/ICC (1/100 - 1/500)

Western Blot (WB) of anti-PPP2R1B antibody

Western blot analysis of PPP2R1B expression in HeLa (A), Raw264.7 (B), H9C2 (C) whole cell lysates.
Rabbit Anti-PPP2R1B Polyclonal Antibody – Cat# MBS821756

Immunofluorescence (IF) of anti-PPP2R1B antibody

Immunofluorescent analysis of PPP2R1B staining in Raw264.7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Rabbit Anti-PPP2R1B Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS821756

Other Notes

Small volumes of anti-PPP2R1B antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PPP2R1B. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A beta isoform isoform d
NCBI Official Synonym Full Names
protein phosphatase 2, regulatory subunit A, beta
NCBI Official Symbol
PPP2R1B
NCBI Official Synonym Symbols
PR65B; PP2A-Abeta
UniProt Protein Name
Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A beta isoform
UniProt Synonym Protein Names
PP2A subunit A isoform PR65-beta; PP2A subunit A isoform R1-beta
UniProt Gene Name
PPP2R1B
UniProt Entry Name
2AAB_HUMAN
NCBI Protein Information
serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A beta isoform; PP2A, subunit A, R1-beta isoform; PP2A, subunit A, PR65-beta isoform; protein phosphatase 2, structural/regulatory subunit A, beta; protein phosphatase 2 (formerly 2A), regulatory subunit A, beta isoform

NCBI Summary for PPP2R1B

This gene encodes a constant regulatory subunit of protein phosphatase 2. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The constant regulatory subunit A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. This gene encodes a beta isoform of the constant regulatory subunit A. Mutations in this gene have been associated with some lung and colon cancers. Alternatively spliced transcript variants have been described. [provided by RefSeq, Apr 2010]
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UniProt Comments for PPP2R1B

PPP2R1B: The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. Belongs to the phosphatase 2A regulatory subunit A family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein phosphatase, regulatory subunit

Chromosomal Location of Human Ortholog: 11q23.2

Cellular Component: lipid raft

Molecular Function: protein binding

Disease: Lung Cancer
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PPP2R1B antibody

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