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Cofilin (CFL1) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS821757

MBS821757 is an antibody targeting CFL1. Also known as Anti-Cofilin Antibody. Reactive in Human, Mouse, Rat, Bovine, Monkey, Pig, Sheep. Host: Rabbit. Rabbit polyclonal antibody to Cofilin More details

$225 / 0.03 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Cofilin (CFL1), Polyclonal Antibody
Full Product Name
Anti-Cofilin Antibody
Product Gene Name
anti-CFL1 antibody
Product Synonym Names
CFL; Cofilin-1; 18 kDa phosphoprotein; p18; Cofilin, non-muscle isoform
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat, Bovine, Monkey, Pig, Sheep
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Specificity
Recognizes endogenous levels of Cofilin protein.
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
Antibody/Peptide Pairs
Cofilin peptide (MBS823197) is used for blocking the activity of Cofilin antibody (MBS821757)
Molecular Weight
18,502 Da
OMIM
601442
3D Structure
ModBase 3D Structure for P23528
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Cofilin. The exact sequence is proprietary.
Applications Tested/Suitable for anti-CFL1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
Application Notes for anti-CFL1 antibody
WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/ICC (1/100 - 1/500)

Western Blot (WB) of anti-CFL1 antibody

Western blot analysis of Cofilin expression in Jurkat (A), HeLa (B), MCF7 (C), A431 (D) whole cell lysates.
Rabbit Anti-CFL1 Polyclonal Antibody – Cat# MBS821757

Immunohistochemistry (IHC) of anti-CFL1 antibody

Immunohistochemical analysis of Cofilin staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Rabbit Anti-CFL1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS821757

Immunofluorescence (IF) of anti-CFL1 antibody

Immunofluorescent analysis of Cofilin staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Rabbit Anti-CFL1 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS821757

Other Notes

Small volumes of anti-CFL1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CFL1. It may not necessarily be applicable to this product.

NCBI GI #
5031635
NCBI GeneID
1072
NCBI Accession #
NP_005498.1
UniProt Primary Accession #
P23528
UniProt Secondary Accession #
Q53Y87; Q9UCA2; B3KUQ1
UniProt Related Accession #
P23528
GenBank Nucleotide #
NM_005507.2
NCBI Official Full Name
cofilin-1
NCBI Official Synonym Full Names
cofilin 1 (non-muscle)
NCBI Official Symbol
CFL1
NCBI Official Synonym Symbols
CFL; HEL-S-15
UniProt Protein Name
Cofilin-1
UniProt Synonym Protein Names
18 kDa phosphoprotein; p18; Cofilin, non-muscle isoform
Protein Family
Cofilin
UniProt Gene Name
CFL1
UniProt Synonym Gene Names
CFL; p18
UniProt Entry Name
COF1_HUMAN
NCBI Protein Information
cofilin-1; p18; 18 kDa phosphoprotein; cofilin, non-muscle isoform; epididymis secretory protein Li 15

NCBI Summary for CFL1

The protein encoded by this gene can polymerize and depolymerize F-actin and G-actin in a pH-dependent manner. Increased phosphorylation of this protein by LIM kinase aids in Rho-induced reorganization of the actin cytoskeleton. Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.[supplied by OMIM, Apr 2004]
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UniProt Comments for CFL1

Cofilin-1: a cytoskeletal protein that controls actin depolymerization. Has a 5-10-fold higher affinity for ATP-actin monomers than cofilin-1. May promote filament assembly rather than disassembly. Two alternatively spliced variants are described. Isoform b is expressed predominantly in skeletal muscle and heart, while isoform a is expressed in various tissues.

Protein type: Cytoskeletal; Nuclear receptor co-regulator; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: extracellular space; cortical actin cytoskeleton; focal adhesion; nuclear matrix; membrane; cytoplasm; intercellular junction; nucleus; vesicle

Molecular Function: protein binding; actin binding

Biological Process: actin filament depolymerization; platelet activation; axon guidance; positive regulation of actin filament depolymerization; response to virus; cytoskeleton organization and biogenesis; negative regulation of cell size; response to amino acid stimulus; protein amino acid phosphorylation; Rho protein signal transduction; neural fold formation; platelet degranulation; cytokinesis after mitosis; establishment of cell polarity; protein import into nucleus; ephrin receptor signaling pathway; innate immune response; regulation of cell morphogenesis; actin cytoskeleton organization and biogenesis; blood coagulation; neural crest cell migration; negative regulation of apoptosis
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Research Articles on CFL1

1. Brain phosphorylated cofilin is downregulated in transgenic mice by RanBP9 overexpression, accelerating amyloid plaque accumulation.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CFL1 antibody

 Reference Product  PubMed Publications
 LIMK1 antibody  >16 publications with CFL1 and LIMK1
 SSH1 antibody  >6 publications with CFL1 and SSH1
 PFN1 antibody  >4 publications with CFL1 and PFN1
 ACTG1 antibody  >2 publications with CFL1 and ACTG1
 ACTB antibody  >2 publications with CFL1 and ACTB

Pathways associated with anti-CFL1 antibody

 Products by Pathway  Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 BDNF Signaling Pathway antibodies  BDNF Signaling Pathway Diagram
 CDC42 Signaling Events Pathway antibodies  CDC42 Signaling Events Pathway Diagram
 CXCR4-mediated Signaling Events Pathway antibodies  CXCR4-mediated Signaling Events Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 EPH-Ephrin Signaling Pathway antibodies  EPH-Ephrin Signaling Pathway Diagram
 EPHB-mediated Forward Signaling Pathway antibodies  EPHB-mediated Forward Signaling Pathway Diagram
 Fc Gamma R-mediated Phagocytosis Pathway antibodies  Fc Gamma R-mediated Phagocytosis Pathway Diagram

Diseases associated with anti-CFL1 antibody

 Disease Name  Pubmed Publications
 Neoplasm Metastasis Antibodies  >9 publications with CFL1 and Neoplasm Metastasis
 Nervous System Diseases Antibodies  >8 publications with CFL1 and Nervous System Diseases
 Breast Neoplasms Antibodies  >7 publications with CFL1 and Breast Neoplasms
 Disease Models, Animal Antibodies  >6 publications with CFL1 and Disease Models, Animal
 Inflammation Antibodies  >5 publications with CFL1 and Inflammation
 Neoplasms, Experimental Antibodies  >3 publications with CFL1 and Neoplasms, Experimental
 Neoplasm Invasiveness Antibodies  >3 publications with CFL1 and Neoplasm Invasiveness
 Cardiovascular Diseases Antibodies  >2 publications with CFL1 and Cardiovascular Diseases
 Memory Disorders Antibodies  >2 publications with CFL1 and Memory Disorders
 Kidney Diseases Antibodies  >1 publications with CFL1 and Kidney Diseases

Organs/Tissues associated with anti-CFL1 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >17 publications with CFL1 and Brain
 Muscle Antibodies  >14 publications with CFL1 and Muscle
 Blood Antibodies  >12 publications with CFL1 and Blood
 Nerve Antibodies  >11 publications with CFL1 and Nerve
 Lung Antibodies  >8 publications with CFL1 and Lung
 Kidney Antibodies  >6 publications with CFL1 and Kidney
 Uterus Antibodies  >5 publications with CFL1 and Uterus
 Vascular Antibodies  >4 publications with CFL1 and Vascular
 Eye Antibodies  >3 publications with CFL1 and Eye
 Embryonic Tissue Antibodies  >3 publications with CFL1 and Embryonic Tissue
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