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POU2F1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS822005

MBS822005 is an antibody targeting POU2F1. Also known as Anti-OCT1 Antibody. Reactive in Human, Chicken, Pig. Host: Rabbit. Rabbit polyclonal antibody to 41913 More details

$225 / 0.03 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
OCT1 (POU2F1), Polyclonal Antibody
Full Product Name
Anti-OCT1 Antibody
Product Gene Name
anti-POU2F1 antibody
Product Synonym Names
OCT1; OTF1; POU domain, class 2, transcription factor 1; NF-A1; Octamer-binding protein 1; Oct-1; Octamer-binding transcription factor 1; OTF-1
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Chicken, Pig
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Specificity
Recognizes endogenous levels of OCT1 protein.
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
Antibody/Peptide Pairs
OCT1 peptide (MBS823072) is used for blocking the activity of OCT1 antibody (MBS822005)
Molecular Weight
72,082 Da
OMIM
164175
3D Structure
ModBase 3D Structure for P14859
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human 41913. The exact sequence is proprietary.
Applications Tested/Suitable for anti-POU2F1 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-POU2F1 antibody
WB (1/500 - 1/1000), IH (1/100 - 1/200)

Western Blot (WB) of anti-POU2F1 antibody

Western blot analysis of OCT1 expression in Jurkat (A), Ramos (B), K562 (C), Molt (D), HeLa (E) whole cell lysates.
Rabbit Anti-POU2F1 Polyclonal Antibody – Cat# MBS822005

Immunohistochemistry (IHC) of anti-POU2F1 antibody

Immunohistochemical analysis of OCT1 staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Rabbit Anti-POU2F1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS822005

Other Notes

Small volumes of anti-POU2F1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for POU2F1. It may not necessarily be applicable to this product.

NCBI GI #
311771667
NCBI GeneID
5451
NCBI Accession #
NP_001185712.1
UniProt Primary Accession #
P14859
UniProt Secondary Accession #
Q5TBT7; Q6PK46; Q8NEU9; Q9BPV1; B1AL91; B1AL93; B4E029; J3KP77
UniProt Related Accession #
P14859
GenBank Nucleotide #
NM_001198783.1
NCBI Official Full Name
POU domain, class 2, transcription factor 1 isoform 2
NCBI Official Synonym Full Names
POU class 2 homeobox 1
NCBI Official Symbol
POU2F1
NCBI Official Synonym Symbols
OCT1; OTF1; oct-1B
UniProt Protein Name
POU domain, class 2, transcription factor 1
UniProt Synonym Protein Names
NF-A1; Octamer-binding protein 1; Oct-1; Octamer-binding transcription factor 1; OTF-1
Protein Family
POU domain, class 2, transcription factor
UniProt Gene Name
POU2F1
UniProt Synonym Gene Names
OCT1; OTF1; Oct-1; OTF-1
UniProt Entry Name
PO2F1_HUMAN
NCBI Protein Information
POU domain, class 2, transcription factor 1; NF-A1; OTF-1; oct-1; octamer-binding protein 1; Octamer-binding transcription factor-1; octamer-binding transcription factor 1

NCBI Summary for POU2F1

The OCT1 transcription factor was among the first identified members of the POU transcription factor family (summarized by Sturm et al., 1993 [PubMed 8314572]). Members of this family contain the POU domain, a 160-amino acid region necessary for DNA binding to the octameric sequence ATGCAAAT.[supplied by OMIM, Jul 2010]
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UniProt Comments for POU2F1

Oct1: a ubiquitous transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and activates the promoters of the genes for some small nuclear RNAs (snRNA) and of genes such as those for histone H2B and immunoglobulins. Modulates transcription transactivation by NR3C1, AR and PGR. Phosphorylation apparently inhibits its interaction with DNA. Four splice variant isoforms have been described. Isoform 2 is lymphocyte specific.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 1q24.2

Cellular Component: nucleoplasm; transcription factor complex; intracellular membrane-bound organelle; nucleus

Molecular Function: protein binding; sequence-specific DNA binding; transcription factor activity

Biological Process: transcription from RNA polymerase III promoter; gene expression; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; olfactory placode formation
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Research Articles on POU2F1

1. a differential control exerted by both Oct-1 and Oct-2 in the transcriptional regulation of the HMGA1 gene

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-POU2F1 antibody

 Reference Product  PubMed Publications
 HCFC1 antibody  >388 publications with POU2F1 and HCFC1
 SP1 antibody  >40 publications with POU2F1 and SP1
 POU2AF1 antibody  >27 publications with POU2F1 and POU2AF1
 SOX2 antibody  >6 publications with POU2F1 and SOX2
 TBP antibody  >4 publications with POU2F1 and TBP
 XRCC5 antibody  >3 publications with POU2F1 and XRCC5
 BRCA1 antibody  >3 publications with POU2F1 and BRCA1
 SNAPC4 antibody  >2 publications with POU2F1 and SNAPC4
 PRKDC antibody  >1 publications with POU2F1 and PRKDC

Pathways associated with anti-POU2F1 antibody

 Products by Pathway  Pathway Diagram
 ATF-2 Transcription Factor Network Pathway antibodies  ATF-2 Transcription Factor Network Pathway Diagram
 Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway antibodies  Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway Diagram
 Calcium Signaling In The CD4+ TCR Pathway antibodies  Calcium Signaling In The CD4+ TCR Pathway Diagram
 FOXA1 Transcription Factor Network Pathway antibodies  FOXA1 Transcription Factor Network Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Glucocorticoid Receptor Regulatory Network Pathway antibodies  Glucocorticoid Receptor Regulatory Network Pathway Diagram
 RNA Polymerase I, RNA Polymerase III, And Mitochondrial Transcription Pathway antibodies  RNA Polymerase I, RNA Polymerase III, And Mitochondrial Transcription Pathway Diagram
 RNA Polymerase III Abortive And Retractive Initiation Pathway antibodies  RNA Polymerase III Abortive And Retractive Initiation Pathway Diagram
 RNA Polymerase III Transcription Pathway antibodies  RNA Polymerase III Transcription Pathway Diagram
 RNA Polymerase III Transcription Initiation Pathway antibodies  RNA Polymerase III Transcription Initiation Pathway Diagram

Diseases associated with anti-POU2F1 antibody

 Disease Name  Pubmed Publications
 Necrosis Antibodies  >14 publications with POU2F1 and Necrosis
 Inflammation Antibodies  >13 publications with POU2F1 and Inflammation
 Nervous System Diseases Antibodies  >8 publications with POU2F1 and Nervous System Diseases
 Kidney Diseases Antibodies  >6 publications with POU2F1 and Kidney Diseases
 Hepatitis Antibodies  >6 publications with POU2F1 and Hepatitis
 Carcinoma, Hepatocellular Antibodies  >4 publications with POU2F1 and Carcinoma, Hepatocellular
 Liver Diseases Antibodies  >4 publications with POU2F1 and Liver Diseases
 Liver Neoplasms Antibodies  >3 publications with POU2F1 and Liver Neoplasms
 Cholestasis Antibodies  >3 publications with POU2F1 and Cholestasis
 Brain Diseases Antibodies  >3 publications with POU2F1 and Brain Diseases

Organs/Tissues associated with anti-POU2F1 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >130 publications with POU2F1 and Blood
 Liver Antibodies  >40 publications with POU2F1 and Liver
 Kidney Antibodies  >33 publications with POU2F1 and Kidney
 Brain Antibodies  >30 publications with POU2F1 and Brain
 Embryonic Tissue Antibodies  >17 publications with POU2F1 and Embryonic Tissue
 Vascular Antibodies  >13 publications with POU2F1 and Vascular
 Lung Antibodies  >13 publications with POU2F1 and Lung
 Spleen Antibodies  >12 publications with POU2F1 and Spleen
 Muscle Antibodies  >11 publications with POU2F1 and Muscle
 Thymus Antibodies  >8 publications with POU2F1 and Thymus
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