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ADARB1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS822051

MBS822051 is an antibody targeting ADARB1. Also known as Anti-ADAR2 Antibody. Reactive in Human, Mouse, Rat, Chicken, Zebrafish. Host: Rabbit. Rabbit polyclonal antibody to ADAR2 More details

$225 / 0.03 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
ADAR2 (ADARB1), Polyclonal Antibody
Full Product Name
Anti-ADAR2 Antibody
Product Gene Name
anti-ADARB1 antibody
Product Synonym Names
ADAR2; DRADA2; RED1; Double-stranded RNA-specific editase 1; RNA-editing deaminase 1; RNA-editing enzyme 1; dsRNA adenosine deaminase
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat, Chicken, Zebrafish
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Specificity
Recognizes endogenous levels of ADAR2 protein.
Preparation and Storage
Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
Antibody/Peptide Pairs
ADAR2 peptide (MBS822775) is used for blocking the activity of ADAR2 antibody (MBS822051)
OMIM
601218
3D Structure
ModBase 3D Structure for P78563
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human ADAR2. The exact sequence is proprietary.
Applications Tested/Suitable for anti-ADARB1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
Application Notes for anti-ADARB1 antibody
WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/ICC (1/100 - 1/500)

Western Blot (WB) of anti-ADARB1 antibody

Western blot analysis of ADAR2 expression in LO2 (A), HepG2 (B), CT26 (C), C6 (D) whole cell lysates. (Predicted band size: 80 kD; Observed band size: 90 kD)
Rabbit Anti-ADARB1 Polyclonal Antibody – Cat# MBS822051

Immunohistochemistry (IHC) of anti-ADARB1 antibody

Immunohistochemical analysis of ADAR2 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Rabbit Anti-ADARB1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS822051

Immunofluorescence (IF) of anti-ADARB1 antibody

Immunofluorescent analysis of ADAR2 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Rabbit Anti-ADARB1 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS822051

Other Notes

Small volumes of anti-ADARB1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ADARB1. It may not necessarily be applicable to this product.

NCBI GI #
4501919
NCBI GeneID
104
NCBI Accession #
NP_001103.1
UniProt Primary Accession #
P78563
UniProt Secondary Accession #
O00395; O00465; O00691; O00692; P78555; A6NFK8; A6NJ84; C3TTQ1; C3TTQ2; C9JUP4; G5E9B4
UniProt Related Accession #
P78563
GenBank Nucleotide #
NM_001112.3
NCBI Official Full Name
double-stranded RNA-specific editase 1 isoform 1
NCBI Official Synonym Full Names
adenosine deaminase, RNA-specific, B1
NCBI Official Symbol
ADARB1
NCBI Official Synonym Symbols
RED1; ADAR2; DRABA2; DRADA2
UniProt Protein Name
Double-stranded RNA-specific editase 1
UniProt Synonym Protein Names
RNA-editing deaminase 1; RNA-editing enzyme 1; dsRNA adenosine deaminase
Protein Family
Double-stranded RNA-specific editase
UniProt Gene Name
ADARB1
UniProt Synonym Gene Names
ADAR2; DRADA2; RED1
UniProt Entry Name
RED1_HUMAN
NCBI Protein Information
double-stranded RNA-specific editase 1; RNA editase; RED1 homolog; RNA-editing enzyme 1; RNA editing deaminase 1; RNA-editing deaminase 1; dsRNA adenosine deaminase DRADA2; adenosine deaminase, RNA-specific, B1 (RED1 homolog rat); adenosine deaminase, RNA-specific, B1 (homolog of rat RED1)

NCBI Summary for ADARB1

This gene encodes the enzyme responsible for pre-mRNA editing of the glutamate receptor subunit B by site-specific deamination of adenosines. Studies in rat found that this enzyme acted on its own pre-mRNA molecules to convert an AA dinucleotide to an AI dinucleotide which resulted in a new splice site. Alternative splicing of this gene results in several transcript variants, some of which have been characterized by the presence or absence of an ALU cassette insert and a short or long C-terminal region. [provided by RefSeq, Jul 2008]
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UniProt Comments for ADARB1

RED1: Editing of the messenger RNAs for glutamate receptor (GluR) subunits by site-selective adenosine deamination. Edits both the GluR-B Q/R and R/G sites efficiently but converts the adenosine in hotspot1 much less efficiently. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding; Nucleolus; Hydrolase; EC 3.5.4.37

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: nucleoplasm; cytoplasm; nucleolus; nucleus

Molecular Function: adenosine deaminase activity; mRNA binding; double-stranded RNA adenosine deaminase activity; protein binding; RNA binding; metal ion binding; double-stranded RNA binding

Biological Process: RNA processing; negative regulation of cell proliferation; base conversion or substitution editing; positive regulation of viral genome replication; regulation of cell cycle; mRNA modification; innate immune response; gene expression; mRNA processing; adenosine to inosine editing; defense response to virus; negative regulation of cell migration
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Research Articles on ADARB1

1. ADAR2-mediated editing of the complementary antisense transcripts is a novel mechanism for regulating the biogenesis of specific miRNAs during hepatocarcinogenesis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-ADARB1 antibody

 Products by Pathway  Pathway Diagram
 C6 Deamination Of Adenosine Pathway antibodies  C6 Deamination Of Adenosine Pathway Diagram
 Formation Of Editosomes By ADAR Proteins Pathway antibodies  Formation Of Editosomes By ADAR Proteins Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 MRNA Editing Pathway antibodies  MRNA Editing Pathway Diagram
 MRNA Editing: A To I Conversion Pathway antibodies  MRNA Editing: A To I Conversion Pathway Diagram

Diseases associated with anti-ADARB1 antibody

 Disease Name  Pubmed Publications
 Lung Neoplasms Antibodies  >2 publications with ADARB1 and Lung Neoplasms
 Cardiovascular Diseases Antibodies  >1 publications with ADARB1 and Cardiovascular Diseases
 Brain Diseases Antibodies  >1 publications with ADARB1 and Brain Diseases
 Hypertension Antibodies  >1 publications with ADARB1 and Hypertension

Organs/Tissues associated with anti-ADARB1 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >4 publications with ADARB1 and Brain
 Prostate Antibodies  >3 publications with ADARB1 and Prostate
 Lung Antibodies  >2 publications with ADARB1 and Lung
 Kidney Antibodies  >1 publications with ADARB1 and Kidney
 Blood Antibodies  >1 publications with ADARB1 and Blood
 Testis Antibodies  >1 publications with ADARB1 and Testis
 Connective Tissue Antibodies  >1 publications with ADARB1 and Connective Tissue
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