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CK1 Epsilon (CSNK1E) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS822054

MBS822054 is an antibody targeting CSNK1E. Also known as Anti-CK1 epsilon Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit. Rabbit polyclonal antibody to CK1 epsilon More details

$225 / 0.03 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
CK1 epsilon (CSNK1E), Polyclonal Antibody
Full Product Name
Anti-CK1 epsilon Antibody
Product Gene Name
anti-CSNK1E antibody
Product Synonym Names
Casein kinase I isoform epsilon; CKI-epsilon; CKIe
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Specificity
Recognizes endogenous levels of CK1 epsilon protein.
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
Antibody/Peptide Pairs
CK1 epsilon peptide (MBS823579) is used for blocking the activity of CK1 epsilon antibody (MBS822054)
Molecular Weight
47,315 Da
OMIM
600863
3D Structure
ModBase 3D Structure for P49674
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CK1 epsilon. The exact sequence is proprietary.
Applications Tested/Suitable for anti-CSNK1E antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
Application Notes for anti-CSNK1E antibody
WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/ICC (1/100 - 1/500)

Western Blot (WB) of anti-CSNK1E antibody

Western blot analysis of CK1 epsilon expression in HEK293T (A), SKBR3 (B) whole cell lysates.
Rabbit Anti-CSNK1E Polyclonal Antibody – Cat# MBS822054

Immunohistochemistry (IHC) of anti-CSNK1E antibody

Immunohistochemical analysis of CK1 epsilon staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Rabbit Anti-CSNK1E Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS822054

Immunofluorescence (IF) of anti-CSNK1E antibody

Immunofluorescent analysis of CK1 epsilon staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Rabbit Anti-CSNK1E Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS822054

Other Notes

Small volumes of anti-CSNK1E antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CSNK1E. It may not necessarily be applicable to this product.

NCBI GI #
583966087
NCBI GeneID
102800317
NCBI Accession #
NP_001276841.1
UniProt Primary Accession #
P49674
UniProt Secondary Accession #
Q9JMK2
UniProt Related Accession #
P49674
GenBank Nucleotide #
NM_001289912.1
NCBI Official Full Name
casein kinase I isoform epsilon
NCBI Official Symbol
LOC400927-CSNK1E
UniProt Protein Name
Casein kinase I isoform epsilon
Protein Family
Casein kinase
UniProt Gene Name
CSNK1E
UniProt Synonym Gene Names
CKI-epsilon; CKIe
UniProt Entry Name
KC1E_HUMAN
NCBI Protein Information
casein kinase I isoform epsilon; CKI-epsilon

NCBI Summary for CSNK1E

This locus represents naturally occurring readthrough transcription between the neighboring LOC400927 (transmembrane phosphoinositide 3-phosphatase and tensin homolog 2 pseudogene) and CSNK1E (casein kinase I isoform epsilon) genes on chromosome 22. The readthrough transcript encodes the same protein as the downstream gene product (casein kinase I isoform epsilon). [provided by RefSeq, Feb 2014]
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UniProt Comments for CSNK1E

CK1E: an ubiquitous protein kinase of the CK1 family. Together with Dvl-1 and Frat-1 activate the Wnt signaling pathway. Central component of the circadian clock. May act as a negative regulator of circadian rhythmicity by phosphorylating PER1 and PER2. May play a role in cell cycle progression. Two splice variant isoforms have been described. Component of the circadian core oscillator, which includes the CRY proteins, CLOCK, or NPAS2, BMAL1 or BMAL2, CK1-D and/or CK1-E, TIMELESS and the PER proteins. Interacts directly with PER1 and PER2 which may lead to their degradation. Interacts with SOCS3. Mutations in hamster and Drosophila orthologs have circadian rhythm phenotypes, and the circadian gene period (per) is a substrate in both human and fly. A coding SNP variant in human, which increases CK1 activity, is negatively associated with circadian disorder. LOF mutations and LOH seen in mammary ductal carcinoma.

Protein type: Protein kinase, CK1; Kinase, protein; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); CK1 group; CK1 family

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: cytoplasm; cytosol; nucleus; ribonucleoprotein complex

Molecular Function: protein serine/threonine kinase activity; protein binding; ATP binding; protein kinase activity

Biological Process: circadian rhythm; Wnt receptor signaling pathway; negative regulation of Wnt receptor signaling pathway; organelle organization and biogenesis; endocytosis; regulation of circadian rhythm; signal transduction; DNA repair; protein amino acid phosphorylation; regulation of cell shape; peptidyl-serine phosphorylation; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; circadian regulation of gene expression; mitotic cell cycle; G2/M transition of mitotic cell cycle
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Research Articles on CSNK1E

1. CKI-epsilon is a potent TDP-43 kinase in vivo and in vitro.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CSNK1E antibody

 Reference Product  PubMed Publications
 PER3 antibody  >12 publications with CSNK1E and PER3
 PER1 antibody  >11 publications with CSNK1E and PER1
 PER2 antibody  >9 publications with CSNK1E and PER2
 CRY1 antibody  >8 publications with CSNK1E and CRY1
 CTNNB1 antibody  >5 publications with CSNK1E and CTNNB1
 ARNTL antibody  >5 publications with CSNK1E and ARNTL
 AXIN1 antibody  >2 publications with CSNK1E and AXIN1
 APC antibody  >1 publications with CSNK1E and APC

Pathways associated with anti-CSNK1E antibody

 Products by Pathway  Pathway Diagram
 Canonical Wnt Signaling Pathway antibodies  Canonical Wnt Signaling Pathway Diagram
 Circadian Rhythm Pathway antibodies  Circadian Rhythm Pathway Diagram
 Circadian Rhythm Pathway antibodies  Circadian Rhythm Pathway Diagram
 Circadian Rhythm Pathway antibodies  Circadian Rhythm Pathway Diagram
 FoxO Family Signaling Pathway antibodies  FoxO Family Signaling Pathway Diagram
 FoxO Signaling Pathway antibodies  FoxO Signaling Pathway Diagram
 Hedgehog Signaling Events Mediated By Gli Proteins Pathway antibodies  Hedgehog Signaling Events Mediated By Gli Proteins Pathway Diagram
 Hedgehog Signaling Pathway antibodies  Hedgehog Signaling Pathway Diagram
 Hedgehog Signaling Pathway antibodies  Hedgehog Signaling Pathway Diagram
 Hippo Signaling Pathway antibodies  Hippo Signaling Pathway Diagram

Diseases associated with anti-CSNK1E antibody

 Disease Name  Pubmed Publications
 Mental Disorders Antibodies  >8 publications with CSNK1E and Mental Disorders
 Nervous System Diseases Antibodies  >3 publications with CSNK1E and Nervous System Diseases
 Neoplasm Metastasis Antibodies  >1 publications with CSNK1E and Neoplasm Metastasis
 Insulin Resistance Antibodies  >1 publications with CSNK1E and Insulin Resistance
 Liver Neoplasms Antibodies  >1 publications with CSNK1E and Liver Neoplasms
 Brain Diseases Antibodies  >1 publications with CSNK1E and Brain Diseases
 Fatty Liver Antibodies  >1 publications with CSNK1E and Fatty Liver
 Kidney Diseases Antibodies  >1 publications with CSNK1E and Kidney Diseases

Organs/Tissues associated with anti-CSNK1E antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >7 publications with CSNK1E and Blood
 Brain Antibodies  >6 publications with CSNK1E and Brain
 Liver Antibodies  >5 publications with CSNK1E and Liver
 Prostate Antibodies  >4 publications with CSNK1E and Prostate
 Nerve Antibodies  >2 publications with CSNK1E and Nerve
 Bone Antibodies  >2 publications with CSNK1E and Bone
 Pharynx Antibodies  >1 publications with CSNK1E and Pharynx
 Ovary Antibodies  >1 publications with CSNK1E and Ovary
 Kidney Antibodies  >1 publications with CSNK1E and Kidney
 Eye Antibodies  >1 publications with CSNK1E and Eye
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